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Sargassum fusiforme polysaccharide functionalized nano-selenium as well as preparation method and application thereof

A hijiki polysaccharide and functionalization technology, applied in the direction of nanotechnology, nanotechnology, nanomedicine, etc., to achieve the effects of good stability, improved antioxidant activity, and high solubility

Inactive Publication Date: 2021-02-19
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Chinese patent CN111406948A discloses a preparation method of nano-selenium, which uses sodium selenite as the selenium source, ascorbic acid as the reducing agent, and centipede algae polysaccharide as the modifier, and prepares a nano-selenium with a stable particle size of about 60nm and strong antioxidant activity. nano-selenium; Chinese patent CN109650349A discloses a preparation method of camellia plant polysaccharide functionalized nano-selenium, which reveals the enhancement effect of camellia plant polysaccharide on the functional properties of nano-selenium; Preparation and activity studies have not been reported yet

Method used

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  • Sargassum fusiforme polysaccharide functionalized nano-selenium as well as preparation method and application thereof
  • Sargassum fusiforme polysaccharide functionalized nano-selenium as well as preparation method and application thereof
  • Sargassum fusiforme polysaccharide functionalized nano-selenium as well as preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The extraction process optimization of embodiment 1 hijiki polysaccharide

[0042] 1. Single factor experiment

[0043] In the experiment of ultrasonic-assisted hot water extraction of hijiki polysaccharides, the following steps were included:

[0044] (1) Grinding the dried hijiki powder with a pulverizer and passing through a 40-mesh sieve, and then refluxing it with ethanol with a concentration of 95% by volume at a temperature of 70°C for 3h (m / v=1:4), After decolorization, the residue was collected by suction filtration, and dried at 50° C. for 12 hours to obtain decolorized hijiki.

[0045] (2) Make the hijiki solution with the decolorized hijiki according to the solid-liquid ratio of 1:20-60g / mL and distilled water, and set the ultrasonic parameters (ultrasonic power 200-600W; ultrasonic time 10-30Min). Ultrasound, and then placed in a 95°C water bath for extraction for 60-180 minutes, and the extract was collected after centrifugation.

[0046] Extraction con...

Embodiment 2

[0075] Example 2 Preparation of hijiki polysaccharide functionalized nano-selenium (SFPS-SeNP)

[0076] Hijiki polysaccharide-nano selenium was prepared by chemical reduction method. Mix the hijiki polysaccharide solution (1 mg / ml) with a concentration of 0.25, 0.5, 1, 2, 3, 4 mg / mL and 0.01 mol / L sodium selenite solution in equal volumes, and mix evenly under the action of magnetic stirring After about 20 minutes, an equal volume of 0.04mol / L ascorbic acid solution was added to the mixed solution, and the reaction was performed under magnetic stirring at 50°C for 4 hours until a stable orange-red solution was formed. The reaction solution was transferred to a dialysis bag with a molecular weight cut-off of 3 kD, dialyzed in a refrigerator at 4°C for 48 hours, and then freeze-dried to obtain hijiki polysaccharide-functionalized nano-selenium complex powder, which was stored in a desiccator. Same as the above operation, the hijiki solution was replaced with an equal volume of ...

Embodiment 3

[0082] The mensuration of embodiment 3 different samples antioxidant activity

[0083] 3.1 The scavenging effect of different samples on DPPH free radicals

[0084] Weigh nano-selenium, hijiki polysaccharide, and hijiki polysaccharide functionalized nano-selenium powder and dissolve them in deionized water to make a sample solution with a certain concentration gradient (0.1, 0.25, 0.5, 0.75, 1, 1.5mg / mL) . Take 150 μL of sample solution and add 150 μL of DPPH solution (0.1 mM, prepared in absolute ethanol), mix well, and react in the dark at room temperature for 30 minutes, measure the absorbance at 517 nm wavelength, replace the sample solution with an equal volume of distilled water as a blank control, etc. volume of absolute ethanol solution instead of DPPH solution as a sample control. The DPPH free radical scavenging rate is calculated according to the following formula:

[0085]

[0086] In the formula: A 0 is the absorbance value of the blank control; A S is the...

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Abstract

The invention discloses sargassum fusiforme polysaccharide functionalized nano-selenium as well as a preparation method and application thereof. The preparation method of the sargassum fusiforme polysaccharide functionalized nano-selenium comprises the following steps: uniformly mixing a sargassum fusiforme polysaccharide solution with sodium selenite by adopting a chemical reduction method, thenadding an ascorbic acid solution, carrying out water bath, and carrying out dialysis and freeze-drying to obtain a compound. The sodium selenite is used as a selenium source, the ascorbic acid is usedas a reducing agent, and the sargassum fusiforme polysaccharide (SFPS) is used as a modifier to prepare a polysaccharide nano-selenium compound (SFPS-SeNPs) with good stability and high solubility. The invention provides a new idea and new development for extraction of the sargassum fusiforme polysaccharide and development of selenium supplementing products. Compared with original nano-selenium particles, the sargassum fusiforme polysaccharide functionalized nano-selenium prepared by the invention has the advantages that the antioxidant activity is improved, and the antioxidant activity is not reduced after the sargassum fusiforme polysaccharide is compounded with the nano-selenium, so that the sargassum fusiforme polysaccharide functionalized nano-selenium is a polysaccharide nano-selenium compound with good stability and high solubility.

Description

technical field [0001] The invention belongs to the technical field of nano-selenium preparation, and in particular relates to hijiki polysaccharide functionalized nano-selenium and its preparation method and application. Background technique [0002] Selenium is an essential trace element for the human body. At the same time, it can help cells resist oxidative damage and also plays an important role in different neurodegenerative diseases. In the body, the main form of selenium is various selenoproteins, which play an important role in various cell redox regulation, detoxification, and immune system protection. However, many beneficial physiological effects of selenium are often dependent on high intake and produce toxicity, which limits its application. Nano-selenium is a high-efficiency and low-toxic form of elemental selenium, which can be selected from a wide range of sources and has excellent biocompatibility. Among the reported methods for controlling nanoparticle g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/04A61K47/61A61P39/06B82Y5/00
CPCA61K33/04A61K47/61A61P39/06B82Y5/00
Inventor 范晓丹王甜赵洪英贲永光刘昶君
Owner SOUTH CHINA UNIV OF TECH
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