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Chlamydomonas reinhardtii, chlamydomonas reinhardtii powder heterotrophic fermentation preparation method and application

A technology of Rhine clothing and algae powder, applied in the field of food processing, achieves high quality standards, eliminates potential safety hazards, and has high safety effects

Active Publication Date: 2021-03-02
山西透云生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is of great practical significance to study a kind of algal raw material that is suitable for the food industry and is rich in nutrition and safety. At present, there is no report on the large-scale production process of Chlamydomonas reinhardtii and its industrial raw materials

Method used

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  • Chlamydomonas reinhardtii, chlamydomonas reinhardtii powder heterotrophic fermentation preparation method and application
  • Chlamydomonas reinhardtii, chlamydomonas reinhardtii powder heterotrophic fermentation preparation method and application
  • Chlamydomonas reinhardtii, chlamydomonas reinhardtii powder heterotrophic fermentation preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Inoculate Chlamydomonas reinhardtii seeds to 6m 3 In the seed tank, the seed tank medium is: copper sulfate 80g, zinc oxalate 80g, sodium acetate 350g, potassium dihydrogen phosphate 200g, calcium hydroxide 800g, magnesium carbonate 10g, iron sulfate 20g, manganese sulfate 20g, ammonia water 0.3L, add water to 4m 3 , adjust the pH to 7.0 with acetic acid, then start the inoculation fermentation, the inoculum size is 0.5vol%. The culture temperature is 20° C., the pressure is 0.05 MPa, the dissolved oxygen is 50%, the stirring speed is 200 rpm, and the pH is maintained between 6.3 and 8.0 during the culture process. When a large amount of foam is produced in the tank, add a defoamer, the defoamer is 3 kg of corn oil and 1 kg of triglycerin, and the amount of defoamer added is 4 kg. During the fermentation process, after three days, 0.2 kg of medium was added every 2 hours. When fermenting for 120h, when the dry weight in the detection culture solution reaches 30g / L, t...

Embodiment 2

[0029]Inoculate Chlamydomonas reinhardtii seeds to 6m 3 In the seed tank, the seed tank medium is: copper sulfate 80g, zinc oxalate 80g, sodium acetate 350g, potassium dihydrogen phosphate 200g, calcium hydroxide 800g, magnesium carbonate 10g, iron sulfate 20g, manganese sulfate 20g, ammonia water 0.3L, add water to 4m 3 , adjust the pH to 7.0 with acetic acid, then start the inoculation fermentation, the inoculum size is 0.5vol%. The culture temperature is 20° C., the pressure is 0.05 MPa, the dissolved oxygen is 50%, the stirring speed is 200 rpm, and the pH is maintained between 6.3 and 8.0 during the culture process. When a large amount of foam is produced in the tank, add a defoamer, the defoamer is 3 kg of corn oil and 1 kg of triglycerin, and the amount of defoamer added is 4 kg. During the fermentation process, after three days, 0.2 kg of medium was added every 2 hours. When fermenting for 120h, when the dry weight in the detection culture solution reaches 30g / L, th...

Embodiment 3

[0033] Inoculate Chlamydomonas reinhardtii seeds to 6m 3 In the seed tank, the seed tank medium is: copper sulfate 80g, zinc oxalate 80g, sodium acetate 350g, potassium dihydrogen phosphate 200g, calcium hydroxide 800g, magnesium carbonate 10g, iron sulfate 20g, manganese sulfate 20g, ammonia water 0.3L, add water to 4m 3 , adjust the pH to 7.0 with acetic acid, then start the inoculation fermentation, the inoculum size is 0.5vol%. The culture temperature is 35° C., the pressure is 0.08 MPa, the dissolved oxygen is 80%, the stirring speed is 600 rpm, and the pH is maintained between 6.3 and 8.0 during the culture process. When a large amount of foam is produced in the tank, add a defoamer, the defoamer is 3 kg of corn oil and 1 kg of triglycerin, and the amount of defoamer added is 4 kg. During the fermentation process, after three days, 0.2 kg of medium was added every 2 hours. When fermenting for 120h, when the dry weight in the detection culture solution reaches 50g / L, t...

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Abstract

The invention provides chlamydomonas reinhardtii and a chlamydomonas reinhardtii powder heterotrophic fermentation preparation method and application.The preparation method of the chlamydomonas reinhardtii powder comprises the following steps that (a) chlamydomonas reinhardtii seeds are inoculated into a seed tank for heterotrophic culture, and after culture is completed, the chlamydomonas reinhardtii seeds are inoculated into a fermentation tank for heterotrophic fermentation, wherein the culture temperature of the seed tank and the culture temperature of the fermentation tank are 15-38 DEG C, the pressure is 0.03-0.08 MPa, the pH value is 5.0-8.0, the dissolved oxygen is 10-100%, and the stirring rotating speed is 0-700 revolutions per minute; (b) after the fermentation is finished, membrane separation is performed on the obtained fermentation liquid to obtain a chlamydomonas reinhardtii solid product; and (c) spray drying is performed on the obtained product to obtain the chlamydomonas reinhardtii powder. The chlamydomonas reinhardtii powder prepared by the method has high-quality nutrient substances such as protein, polysaccharide, unsaturated fatty acid and vitamin, is high insafety and has a remarkable blood sugar reducing effect.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a heterooxygen fermentation preparation method and application of Chlamydomonas reinhardtii and Chlamydomonas reinhardtii powder. Background technique [0002] Diabetes mellitus is a group of chronic metabolic diseases characterized by chronic glucose metabolism disorders and hyperglycemia due to insulin secretion defects and (or) insulin biological action disorders. Prolonged illness can cause multi-system damage, leading to chronic progressive lesions in the eyes, kidneys, nerves, heart, blood vessels and other tissues, causing functional defects and failure. In the past decade, the prevalence of diabetes has been increasing all over the world. With the improvement of people's living standards and the aging of society, diabetes has become a worldwide non-communicable epidemic disease. At present, there are about 137 million diabetic patients in the world, and nearly 4...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12A23L33/10C12R1/89
CPCC12N1/12A23L33/10A23V2002/00A23V2200/328A23V2200/30
Inventor 牛志国周萍赵元芬王力平王照雪吴会广
Owner 山西透云生物科技有限公司
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