A strain of Paenibacillus peirui and its application in the control of cucumber bacterial soft rot
A bacillus and bacteria technology, which is applied to Paenibacillus pierreus and its application field in the prevention and control of cucumber bacterial soft rot, can solve the problems of high speed of transmission, environmental pollution, and wide range of harm.
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Embodiment 1
[0059] Example 1, Isolation, Screening, Identification and Preservation of Paenibacillus russica ZF390
[0060] 1. Isolation of strains
[0061] Bacterial strains were isolated from potato rhizosphere soils in Heilongjiang, Sichuan, Hebei, Inner Mongolia and Shandong provinces, and a total of 1200 pure cultured bacterial strains were isolated.
[0062] The specific method is as follows: Weigh 10 g of potato rhizosphere soil, dissolve it in 90 mL of sterile water, and shake it at 28 °C and 180 r / min for 30 min to make it fully mixed; -2 to 10 -5 Carry out gradient dilution, draw 100 μL of the diluted suspension to coat LB solid medium plates (3 plates for each dilution), and after 2 days of constant temperature cultivation at 28°C, pick bacterial colonies with different shapes on the LB solid medium Streak purification on the plate, and then numbered and saved.
[0063] 2. Strain screening
[0064] 1. In vitro screening
[0065] Test bacteria: 1200 bacterial strains isolat...
Embodiment 2
[0100] Embodiment 2, Paenibacillus russia ZF390 antibacterial spectrum determination of Peirui
[0101] 1. Determination of fungal inhibition spectrum
[0102] The inhibitory rate of Paenibacillus peirui ZF390 to various pathogenic fungi was detected by plate confrontation method.
[0103] The pathogenic fungi are: Corynespora multimata, Fusarium oxysporum, capsicum anthracnose, Phytophthora capsici, Puccinia solani, Bispora watermelon, Botrytis cinerea or Rhizoctonia solani.
[0104] The specific method is as follows: Pick a single colony of Paenibacillus peirui ZF390 and inoculate it in LB liquid medium, and culture it with shaking at 28°C and 180r / min until the concentration is 1×10 8 cfu / mL, which is the ZF390 bacterial suspension; inoculate a pathogenic fungus cake with a diameter of 5 mm in the center of the PDA medium plate, and at the same time inoculate 5 μL of the ZF390 bacterial suspension at a place 3.5 cm away from the central plate (use LB liquid medium as the Z...
Embodiment 3
[0133] Embodiment 3, the enzyme activity assay of Paenibacillus russica ZF390
[0134] 1. Pick a single colony of Paenibacillus peirui ZF390 and inoculate it in LB liquid medium, and culture it with shaking at 28°C and 180r / min until the bacterial concentration is 1×10 8 cfu / mL is the ZF390 bacterial suspension.
[0135] 2. Take 5 μL of ZF390 bacterial suspension and inoculate it in the center of the CMC medium plate, and incubate at 28°C for 24 hours.
[0136] CMC medium: 0.1 g of magnesium sulfate, 1 g of ammonium sulfate, 10× phosphate buffer (70 g of dipotassium hydrogen phosphate, 20 g of potassium dihydrogen phosphate, 1000 mL of distilled water) 100 mL, 5 g of yeast extract, 2 mL of glycerol, sodium carboxymethyl cellulose 1 g, 8 g of agar (bacto agar), 900 mL of distilled water.
[0137] 3. Take 5 μL of ZF390 bacterial suspension and inoculate it in the center of the protease medium plate, incubate at 28°C for 24 hours, and then measure the diameter of the transparen...
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