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Serum/plasma metabolism molecular marker related to auxiliary diagnosis of intrahepatic cholestasis in gestation period and application of serum/plasma metabolism molecular marker

A molecular marker, cholestasis technology, applied in the fields of metabolomics and reproductive medicine, to achieve the effect of high diagnostic value, convenient and easy diagnosis

Inactive Publication Date: 2021-06-04
WUXI MATERNAL & CHILD HEALTH HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are currently no reports of relatively stable metabolic molecular markers for ICP-assisted diagnosis. If ICP-specific or abnormally expressed metabolites can be screened out as molecular markers and corresponding auxiliary diagnostic kits can be developed, it will be greatly improved. Improving the Diagnosis Status of ICP in my country

Method used

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  • Serum/plasma metabolism molecular marker related to auxiliary diagnosis of intrahepatic cholestasis in gestation period and application of serum/plasma metabolism molecular marker
  • Serum/plasma metabolism molecular marker related to auxiliary diagnosis of intrahepatic cholestasis in gestation period and application of serum/plasma metabolism molecular marker
  • Serum/plasma metabolism molecular marker related to auxiliary diagnosis of intrahepatic cholestasis in gestation period and application of serum/plasma metabolism molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Collection of research samples and data arrangement

[0040] The inventor collected a large number of peripheral blood samples and placental tissue samples from Wuxi Maternal and Child Health Hospital Affiliated to Nanjing Medical University and healthy control pregnant women from March 2017 to October 2018 (the samples used for research were collected during the same period, sampling, Uniform packaging and storage conditions), through the arrangement of the sample data, the inventor selected 100 samples that meet the following standards as experimental samples for metabolomics detection and verification based on the UPLC-Q active MS method:

[0041] (1) Included case group: The diagnostic criteria of ICP refer to the guidelines for the diagnosis and treatment of ICP patients (first edition), and the specific criteria are as follows: 1) Skin itching occurs in the second and third trimesters of pregnancy, or accompanied by varying degrees of jaundice; 2) Laborat...

Embodiment 2I

[0045] Example 2 ICP patients differential metabolic profile analysis

[0046] The above-mentioned 4 eligible ICP cases and 4 healthy controls were tested by metabolomics to obtain relevant results. The specific experimental method is as follows:

[0047] 1.1 Instrument

[0048]UPLC Ultimate 3000system (Dionex) high performance liquid chromatography; Q-Exactive triple quadrupole tandem mass spectrometer; TraceFinder 3.1 (Thermo Fisher Scientific); Simca P 13.0 (Umetrics, Sweden); XW-80A vortex mixer (Shanghai Qingpu Hu West Instrument Factory); electronic balance (MettlerAE240); KQ3200B ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

[0049] 1.2 Reagents

[0050] 500 metabolite standards (≥98.0%, Sigma–Aldrich, St.Louis, MO, USA); methanol, acetonitrile (≥99.9%, Merck, German); formic acid (≥98.0%, Sigma–Aldrich, St.Louis , MO, USA); n-hexane (chromatographic grade, ≥98.0%, Aladdin Reagent (Shanghai) Co., Ltd.); chloroform (≥99.9%, Alfa Aesar (China) Chemica...

Embodiment 3

[0066] Example 3 Verification of the expression of ICP serum / plasma metabolic molecular markers based on the UPLC-Q exact MS method

[0067] According to the detection method of UPLC-Q active MS in Example 2, the inventors detected palmitoylcarnitine and ACOX1 in the serum samples of 50 ICP patients and 50 healthy control groups in an independent population, and drew ROC curves accordingly. The demographic characteristics, TBA (μmol / L) level and the average expression levels of metabolic molecular markers palmitoylcarnitine (log mass spectrum peak area) and ACOX1 (pg / ml) in serum / plasma samples were compared by student t test. difference in distribution. Statistical analysis was performed using SPSS16.0 statistical analysis software. The statistical significance level P value was set at 0.05, and all statistical tests were two-sided. The results are shown in Table 1. According to the verification experiments of palmitoylcarnitine and ACOX1 in serum, the inventors detected t...

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Abstract

The invention discloses a serum / plasma metabolism molecular marker related to auxiliary diagnosis of intrahepatic cholestasis in a gestation period and application of the serum / plasma metabolism molecular marker. The invention relates to a serum / plasma metabolism molecular marker related to ICP auxiliary diagnosis, which is composed of palmitoyl carnitine or palmitoyl carnitine and ACOX1. The invention is to develop a group of high-specificity and high-sensitivity metabolic molecular markers highly related to ICP morbidity by separating and researching ICP cases of primary birth and single birth pregnancy and metabolic molecular markers in serum / plasma of healthy pregnant women matched with the ICP cases of primary birth and single birth pregnancy, and provides laboratory support for screening, diagnosis and treatment of ICP.

Description

[0001] field of invention [0002] The invention belongs to the field of metabolomics and reproductive medicine, and relates to a serum / plasma metabolic molecular marker related to the auxiliary diagnosis of intrahepatic cholestasis during pregnancy and an application thereof. Background technique [0003] Intrahepatic cholestasis of pregnancy (ICP) is an idiopathic liver disease that seriously endangers the health of mother and child during pregnancy. It usually occurs in the second and third trimesters of pregnancy. Elevation is the main feature. The harm of the disease is mainly to cause fetal distress, spontaneous premature birth, stillbirth, etc., and increase the perinatal morbidity and mortality. The incidence of ICP is as high as 1.2%. At the same time, some studies have pointed out that the perinatal mortality rate is about 6-10 times that of normal pregnancy. Early detection and intervention of ICP can effectively reduce the maternal and infant complications caused...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 张婷罗亮董蕊锐赵绍杰叶宁珍姚婷婷王晶邹萍王高莹李娜陈敏健邹少晗周从华
Owner WUXI MATERNAL & CHILD HEALTH HOSPITAL
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