Method for synthesizing L-lactide from chiral L-lactic acid produced by using lignocellulose biomass as raw material

A technology of lignocellulose and biomass, which is applied in the field of producing chiral L-lactic acid and synthesizing L-lactide, can solve the problems of difficult raw materials, low utilization rate, low yield and yield of cellulose lactic acid, and achieve high chirality High degree, significant economic benefits, and the effect of reducing the difficulty of operation

Pending Publication Date: 2021-06-11
SHANXI SYNTHETIC BIOLOGY INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cellulose chiral L-lactic acid synthetic L-lactide that is produced with lignocellulose as raw material mainly has the following obstacles: (1) inhibitors (furfural, 5-hydroxymethylfurfural, vanilla aldehyde and syringaldehyde, etc.) will seriously inhibit the growth and lactic acid fermentation performance of fermentation strains, and the residual inhibitors make the purification of polymerization grade L-lactic acid very difficult; (2) cellulosic lactic acid fermentation strains account for 40% of total

Method used

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  • Method for synthesizing L-lactide from chiral L-lactic acid produced by using lignocellulose biomass as raw material
  • Method for synthesizing L-lactide from chiral L-lactic acid produced by using lignocellulose biomass as raw material
  • Method for synthesizing L-lactide from chiral L-lactic acid produced by using lignocellulose biomass as raw material

Examples

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Effect test

Embodiment 1

[0046] Example 1: A method for synthesizing L-lactide using chiral L-lactic acid produced from wheat straw as raw material 1. Ultimate biodegradation of pretreated wheat straw to remove inhibitors

[0047] After the wheat straw is pretreated with dry dilute acid, the pH of the material is adjusted to 5 with 20% (w / w) calcium hydroxide or calcium carbonate, and 10% (w / w) of Cladosporium Amorphotheca resinae is added For ZN1 seeds, 3 kg of uniformly mixed wheat straw and Cladosporium resinifera seeds were loaded into a 15L bioreactor for solid-state biodegradation under the conditions of aeration rate of 0.5vvm and temperature of 28°C. During the biodegradation process, the content of the main inhibitors in the material was determined by high performance liquid chromatography. At 36 hours, furfural, 5-hydroxymethylfurfural, vanillin and syringaldehyde were completely consumed.

[0048] 2. Using high solid content pretreatment and detoxified wheat straw to ferment cellulose L-lac...

Embodiment 2

[0059] Example 2: A method for synthesizing L-lactide from chiral L-lactic acid produced from corn stalks as raw material 1. Final biodegradation of pretreated corn stalks to remove inhibitors

[0060] After corn stalks were pretreated with dry dilute acid, the pH of the material was adjusted to 4.8 with 25% (w / w) calcium hydroxide or calcium carbonate, and the cultivated Paecilomyces variotii FN89 (CGMCC17665) seeds were Inoculate in the material at a ratio of 20% (w / w), mix evenly, take 4kg of corn stalks and Cladosporium resinifera seeds mixture and put it into a plastic box, and carry out static biodegradation in a constant temperature incubator (37°C). During the biodegradation process, high performance liquid chromatography was used to measure the content of main inhibitors in the materials. After testing, furfural, 5-hydroxymethylfurfural, vanillin and syringaldehyde were completely consumed within 48 hours.

[0061] 2. Using 30% (w / w) solid content pretreatment and det...

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Abstract

The invention belongs to the field of biorefinery, and relates to a method for synthesizing L-lactide by producing chiral L-lactic acid from a lignocellulose raw material. The method comprises the following steps: (1) removing an inhibitor from a pretreated lignocellulose raw material in an ultimate biodegradation manner; (2) performing high-solid-content synchronous saccharification and co-fermentation on the lignocellulose raw material to obtain cellulose L-lactic acid with high concentration, high chirality and extremely low residual sugar; (3) preparing polymer grade L-lactic acid; and (4) carrying out dehydration, polycondensation, depolymerization and purification on the purified cellulose chiral L-lactic acid to obtain L-lactide. According to the method disclosed by the invention, the influence of an inhibitor on L-lactic acid fermentation and L-lactic acid purity is relieved by adopting a biodegradation method, high-concentration and extremely low-residual-sugar cellulose chiral L-lactic acid is obtained by adopting a high-solid-content total-sugar co-fermentation method, and the cellulose chiral L-lactic acid is subjected to the steps of purification, polycondensation, depolymerization and the like; the synthesis of the L-lactide from the lignocellulose raw material is realized.

Description

technical field [0001] The invention relates to the field of biorefinery, in particular to a research on the production of chiral L-lactic acid and the synthesis of L-lactide by using renewable resource lignocellulosic biomass raw materials. Background technique [0002] Polylactic acid (PLA) is a biodegradable plastic that has been widely used as a substitute for petroleum-derived polymers in packaging materials, biomedical materials, and other fields. The synthesis of polylactic acid is mainly achieved through a two-step method. First, chiral lactic acid monomers are polycondensed into low-molecular-weight polylactic acid, and then depolymerized to obtain lactide; then, lactide is used as a polymerization monomer to polymerize high-molecular-weight polylactic acid. The synthesis of lactide is the key step of polylactic acid. At present, lactide is mainly synthesized by using chiral lactic acid obtained by fermentation of starch raw materials, and its production cost and f...

Claims

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Application Information

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IPC IPC(8): C12P7/56C08G63/08C12R1/01
CPCC12P7/56C08G63/08C12P2201/00C12P2203/00
Inventor 鲍杰贾佳何妮玲邱忠洋
Owner SHANXI SYNTHETIC BIOLOGY INST CO LTD
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