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Mineralized fiber reinforced three-dimensional porous scaffold as well as preparation method and application thereof

A three-dimensional porous, fiber-reinforced technology used in tissue engineering and biomedical materials to achieve easy access, improved mechanical properties and biocompatibility

Active Publication Date: 2021-06-25
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on the use of mineralized fibers to reinforce composites

Method used

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  • Mineralized fiber reinforced three-dimensional porous scaffold as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Prepare regenerated silk fibroin (RSF) aqueous solution, and concentrate it to RSF mass fraction of 10% for use; 0.5~3mg / mL graphene oxide (GO) dispersion is blended with RSF aqueous solution to control the relationship between GO and RSF. The mass ratio was 1:100, and the blended mixed solution was concentrated to 30% RSF mass fraction; RSF / GO composite fiber mat was obtained by electrospinning. Among them, the experimental parameters of electrospinning are: relative humidity is 40±5%, temperature is 22±5°C, inner diameter of needle is 0.6mm, voltage is 20KV, receiving distance from needle to receiver (aluminum foil) is 10cm, micro-injection pump The extrusion speed is 1.2mL / h.

[0031] The RSF / GO composite fiber mat was post-treated with 80 vol.% ethanol aqueous solution for 1 h, then taken out and dried, and the RSF / GO fiber was sheared at high speed by a homogenizer to prepare short fibers.

[0032] The treatment solution is a mixed solution of chitosan (CS), RSF a...

Embodiment 2

[0035] The difference between embodiment 2 and embodiment 1 is: embodiment 2 controls the regenerated silk fibroin (RSF) aqueous solution mass fraction to be 20%, the mass ratio of GO and RSF is 1:50, and treatment liquid is chitosan, silk The mixed solution of plain protein, the mineralization time was 15 days, the mass ratio of sodium alginate and agarose was 2:1, and the mass ratio of RSF / GO composite fiber mat to sodium alginate / agarose was 1:50. And embodiment 1 controls the mass fraction of RSF solution to be 10%, the mass ratio of GO and RSF is 1:100, dipping solution is the mixed solution of chitosan, silk fibroin, mineralization time is 7 days, sodium alginate and agar The mass ratio of sugar is 3:1, and the mass ratio of RSF / GO composite fiber mat to sodium alginate / agarose is 1:100, and other operations are the same as in Example 1, as follows:

[0036] An aqueous solution of regenerated silk fibroin (RSF) was prepared and concentrated until the mass fraction of RSF...

Embodiment 3

[0041] The difference between Example 3 and Example 2 is: Example 3 controls the mass ratio of GO and RSF to be 1:20, the treatment solution is a mixed solution of silk fibroin and dopamine, the mineralization time is 30 days, sodium alginate The mass ratio to agarose is 1:1, and the mass ratio of RSF / GO composite fiber mat to sodium alginate / agarose is 1:20. While embodiment 2 controls the mass ratio of GO and RSF to be 1:50, the treatment solution is a mixed solution of chitosan, silk fibroin and dopamine, the mineralization time is 15 days, and the mass ratio of sodium alginate and agarose is 2 : 1, the mass ratio of fiber and sodium alginate / agarose is 1:50, and other operations are identical with embodiment 2, as follows:

[0042] An aqueous solution of regenerated silk fibroin (RSF) was prepared and concentrated until the mass fraction of RSF was 20% for use. Blend 0.5~3mg / mL graphene oxide (GO) dispersion liquid with RSF aqueous solution, control the mass ratio of GO a...

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Abstract

The invention relates to the technical field of medical materials, and discloses a mineralized fiber reinforced three-dimensional porous scaffold and a preparation method and application thereof. The preparation method comprises the steps: 1, blending a silk fibroin aqueous solution and a graphene oxide dispersion solution; 2) preparing a silk fibroin / graphene oxide composite fiber felt; (3) carrying out post-treatment on the silk fibroin / graphene oxide composite fiber felt by utilizing an ethanol water solution; 4) performing high-speed shearing on the silk fibroin / graphene oxide composite fiber felt to prepare short fibers; 5) dipping the short fibers in the treating fluid for a period of time, taking out and drying; 6) immersing the silk fibroin / graphene oxide composite fiber into a mineralizing solution; 7) preparing a sodium alginate / agarose mixed solution; 8) mixing the mineralized short fibers with a sodium alginate / agarose solution; 9) freezing the mixed solution in the step (8) into a solid in a low-temperature refrigerator, and then performing freeze-drying. The three-dimensional porous scaffold prepared by the invention has good biocompatibility and excellent mechanical properties.

Description

technical field [0001] The invention relates to the technical fields of tissue engineering and biomedical materials, in particular to a mineralized fiber-reinforced three-dimensional porous scaffold and its preparation method and application. Background technique [0002] The traditional autologous bone grafting technology has always been the best way to repair bone tissue. However, because the range of autologous bone materials is very limited, it will cause secondary damage to the human body. Therefore, in order to cope with various unpredictable bone tissue injuries, alternative artificial bone materials have been developed rapidly. There are many bone tissue scaffold materials currently used in biomedicine, such as hydroxyapatite, chitosan, silk fibroin, polycaprolactone, and the like. Among them, sodium alginate is easy to extract from the kelp of brown algae. It is a natural polysaccharide, and its aqueous solution has a certain viscosity and is easy to form agglomera...

Claims

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Application Information

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IPC IPC(8): A61L27/44A61L27/56A61L31/12A61L31/14D01F4/00D01F1/10D06M13/144D06M13/335D06M15/03D06M15/15D06M101/10
CPCD01F4/00D01F1/10D06M13/144D06M13/335D06M15/03D06M15/15A61L27/443A61L27/56A61L31/127A61L31/146D06M2101/10A61L2430/02C08L5/04
Inventor 周志承张超叶玮王心如
Owner HUAIYIN INSTITUTE OF TECHNOLOGY