Preparation method and application of biodegradable self-adhesive hydrogel
A biodegradable, hydrogel technology, used in applications, drug delivery, tissue regeneration, etc., can solve problems such as poor biodegradability, achieve low cost, good UV resistance, and accelerate wound healing.
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Embodiment 1
[0052] A preparation method of biodegradable self-adhesive hydrogel, comprising the following steps:
[0053] Step 1: 10 g of gelatin from pig skin was dissolved in 200 ml of phosphate buffer (PH=7.4) at a temperature of 60° C., and stirred for 30 min. Then 8ml of methacrylic anhydride was slowly added at 0.5ml / min, and the mixture was stirred in a 50°C water bath for 3h. To terminate the methacryloyl modification reaction, the solution was diluted with 200 ml of phosphate buffer. Thereafter, the solution was put into a dialysis bag (MW=14000), and dialyzed for 7 days at 37°C. The dialyzed liquid was lyophilized to obtain methacrylic anhydride gelatin.
[0054] Step 2: Dissolve 0.1 g of gallic acid in 10 ml of deionized water, and stir in a 35°C water bath for 10 min, then add 300 μl of NaOH (0.5 g / ml), and continue stirring for 20 min to obtain a gallic acid prepolymer.
[0055] Step 3: Take 1.5 g of methacrylic anhydride gelatin obtained in step 1 and dissolve it in 8 ml ...
Embodiment 2
[0057] Step 1: 10 g of gelatin from pig skin was dissolved in 200 ml of phosphate buffer (PH=7.4) at a temperature of 60° C., and stirred for 30 min. Then 8ml of methacrylic anhydride was slowly added at 0.5ml / min, and the mixture was stirred in a 50°C water bath for 3h. To terminate the methacryloyl modification reaction, the solution was diluted with 200 ml of phosphate buffer. Thereafter, the solution was put into a dialysis bag (MW=14000), and dialyzed for 7 days at 37°C. The dialyzed liquid was lyophilized to obtain methacrylic anhydride gelatin.
[0058] Step 2: Dissolve 0.2 g of dopamine in 10 ml of deionized water, and stir in a 35°C water bath for 10 min, then add 300 μl of NaOH (0.5 g / ml), and continue stirring for 20 min to obtain a dopamine prepolymer.
[0059] Step 3: Dissolve 1.5 g of the methacrylic anhydride gelatin obtained in step 1 in 8 ml of water, and add 2 ml of the dopamine prepolymer solution obtained in step 2 to the solution, and then add 100 μl of ...
Embodiment 3
[0061] Step 1: A 1 wt% chitosan solution was prepared by dissolving 1 g of chitosan in 99 ml of deionized water, and then 1 ml of acetic acid was added to the solution at a constant rate. 4 ml of methacrylic anhydride was added to the solution and stirred overnight at room temperature. Thereafter, the solution was put into a dialysis bag (MW=14000), and dialyzed for 7 days at 37°C. The dialyzed liquid was freeze-dried to obtain methacrylic anhydride chitosan.
[0062] Step 2: Dissolve 0.1 g of tannic acid in 10 ml of deionized water, and stir in a 35° C. water bath for 10 min, then add 300 μl of NaOH (0.5 g / ml), and continue stirring for 20 min to obtain a tannic acid prepolymer.
[0063] Step 3: Dissolve 0.5 g of methacrylic anhydride chitosan obtained in step 1 in 8 ml of water, and add 2 ml of the tannic acid prepolymer solution obtained in step 2 to the solution, and add 100 μl of polyethylene glycol (diol) successively Diacrylate, 0.2 g of sodium persulfate and 20 μl of...
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