High-strength polysaccharide-MXene composite microsphere and preparation method thereof

A composite microsphere, high-strength technology, applied in the preparation of microspheres, microcapsule preparations, etc., can solve the problems of long separation time, difficulty in large-scale separation and preparation, and low mechanical strength

Pending Publication Date: 2021-09-10
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the low mechanical strength of the current polysaccharide separation medium, which can only be operated at a low flow rate, the separation time is too long, resulting in the inactivation of biological macromolecules and the difficulty of large-scale separation and preparation of proteins. The present invention uses MXene and polysaccharide molecules to carry out Composite, the -OH / -O- on the surface of Mxene can form hydrogen bonds with the -O- / -OH on the polysaccharide molecular chain, which acts as an additional physical cross-linking point, supplemented by subsequent simple chemical cross-linking, to prepare high-strength polysaccharides -MXene composite microspheres, which have great application potential in the fields of biocatalysis and fast-flow protein chromatographic separation

Method used

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  • High-strength polysaccharide-MXene composite microsphere and preparation method thereof
  • High-strength polysaccharide-MXene composite microsphere and preparation method thereof
  • High-strength polysaccharide-MXene composite microsphere and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) First weigh dextran T40 (7.5g) and dissolve it in 20ml ultrapure water, then weigh MXene (0.15g) and add it to 5ml deionized water, disperse evenly with a hand-held homogenizer, and finally mix the dextran solution and Add the MXene dispersion into a 50ml beaker and stir evenly with magnetic force. In a 250ml three-necked flask equipped with mechanical stirring, add chlorobenzene (75ml) and Span 80 (2.4g) and heat up to 30°C as an oil phase. Add the above-prepared water phase into the oil phase for emulsification at 150 rpm for 30 min to obtain a gray W / O emulsion.

[0027] 2) Slowly add a mixed solution of 5ml of cross-linking agent glutaraldehyde and 0.75ml of concentrated hydrochloric acid dropwise to the above emulsion, and further carry out cross-linking reaction at 30°C. spheres to obtain dextran-MXene composite microspheres with an average particle size of 90 μm. After crosslinking, the composite microspheres were stored in 20% ethanol at 4°C.

Embodiment 2

[0029]1) First weigh agarose (0.6g) and 10ml of ultrapure water into a 100ml round bottom flask, heat with magnetic stirring at 90°C until the solution is clear and transparent, then weigh MXene (0.06g) and add it to 5ml of deionized water, Disperse evenly with a hand-held homogenizer, and finally mix the MXene dispersion and agarose solution evenly as the water phase. In a 250 ml three-neck flask equipped with mechanical stirring, add liquid paraffin (70 ml), petroleum ether (30 ml), Span 80 (3.6 g) and Tween (0.4 g) and heat up to 70 ° C as the oil phase. Add the above-prepared water phase into the oil phase for emulsification at 400 rpm for 30 min to obtain a gray W / O emulsion.

[0030] 2) Slowly add a mixed solution of 4ml of cross-linking agent ethylene glycol diglycidyl ether and 2ml of 50% KOH dropwise to the above emulsion, further carry out cross-linking reaction at 70°C, react for 8 hours and then use cyclohexane, ethanol and Wash the microspheres with deionized wat...

Embodiment 3

[0032] 1) First weigh cellulose (1.0g) and 20ml of cellulose dissolving solution (sodium hydroxide: urea: water = 7: 12: 81) ultrapure water and put it into a 100ml round bottom flask as the water phase. Magnetically stir and heat until the solution is clear and transparent, then weigh MXene (0.05g) and add it to 5ml deionized water, disperse evenly with a hand-held homogenizer, and finally mix the MXene dispersion and cellulose solution evenly as the water phase. In a 250ml three-neck flask equipped with mechanical stirring, add olive oil (100ml) and hydroxyethyl cellulose (4g) and heat up to 90°C as the oil phase. Add the above-prepared water phase into the oil phase for emulsification at 1000 rpm for 50 min to obtain a gray W / O emulsion.

[0033] 2) Slowly add a mixed solution of 6ml of cross-linking agent 1,4-butanediol diglycidyl ether and 2ml of 40% NaOH dropwise to the above emulsion, further carry out the cross-linking reaction at 70°C, and react with cyclohexane in tu...

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Abstract

The invention relates to the field of preparation of natural polymer/inorganic nanosheet composite materials, and especially relates to a high-strength polysaccharide-MXene composite microsphere and a preparation method thereof. The surface of MXene is rich in hydroxyl groups, hydrogen bonds can be formed through -OH/-O- and -O-/-OH on a polysaccharide molecular chain, an additional physical cross-linking point effect is achieved, subsequent chemical cross-linking is assisted, and the high-strength polysaccharide-MXene composite microsphere is prepared. Protein adsorption and pressure flow velocity curve experiments show that the composite microsphere is good in biocompatibility, the operation flow velocity is greatly improved compared with that of a pure polysaccharide microsphere, and the composite microsphere has great application potential in the fields of biological catalysis and high-flow-velocity egg white spectrum separation.

Description

technical field [0001] The invention relates to the field of preparation of natural polymer / inorganic nanosheet composite materials, in particular to a high-strength polysaccharide-MXene composite microsphere and a preparation method thereof. Background technique [0002] In the fields of modern life technology such as medical treatment, artificial immunization, and gene diagnosis, the rapid and large-scale separation and preparation of biological macromolecules such as proteins, nucleic acids, and virus vaccines is a bottleneck problem that needs to be solved urgently, because these biological macromolecules must be highly separated and purified before they can be applied. . Liquid chromatography has mild separation conditions and high resolution, and plays an irreplaceable role in the separation and purification of biomacromolecules. The separation performance of liquid chromatography depends on the performance of the separation medium. At present, the mainstream biomacro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02B01J13/14
CPCB01J13/02B01J13/14
Inventor 曲剑波陈梦琦李玥
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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