Manganese metal organic framework (MOF) biological composite material and application thereof in detection of food-borne pathogenic bacteria

A composite material, organic framework technology, applied in the direction of analyzing materials, material resistance, material electrochemical variables, etc., can solve the problems of low conductivity, interference, reduced detection sensitivity and accuracy, etc.

Inactive Publication Date: 2021-09-10
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are currently two main difficulties in the application of MOF materials as electrochemical probes: (1) Most MOFs have low electrical conductivity, so it is necessary to dope materials with conductive substances (graphene, tin oxide, biological macromolecular enzymes, etc.) These modification strategies of electrochemical signals will introduce additional electrical signal interfering ions, which interfere with signal acquisition during actual sample detection, thereby reducing detection sensitivity and accuracy
In addition, MOF is a good adsorption material, but it lacks selective adsorption capacity. During the detection process, it is easy to adsorb and capture the target and impurities together, resulting in detection errors.

Method used

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  • Manganese metal organic framework (MOF) biological composite material and application thereof in detection of food-borne pathogenic bacteria
  • Manganese metal organic framework (MOF) biological composite material and application thereof in detection of food-borne pathogenic bacteria
  • Manganese metal organic framework (MOF) biological composite material and application thereof in detection of food-borne pathogenic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1, preparation of manganese metal organic framework biocomposite material

[0044] The schematic flow chart of the preparation method is as figure 1 shown.

[0045] (1) Preparation of MnCl 2 4H 2O (439.2mg, 2.22mM, 3.3Equiv) and 2,5-dihydroxyterephthalic acid (DHTP, 133.2mg, 0.672mM, 1Equiv) in 60mL of DMF-methanol-water (1:1:15, V / V / V) mixed solvent for 20min. The mixed solution was transferred to a 100mL polytetrafluoroethylene reactor sleeve, crystallized at 140°C for 24 hours, dried after centrifugal purification, and the product was brownish yellow solid powder (spherical structure under the electron microscope).

[0046] The TEM figure, XPS characterization figure, XRD characterization figure and FTIR characterization figure of the Mn-MOF-74 nanometer microspheres prepared in this embodiment are as follows figure 2 (a), figure 2 (b), figure 2 (c) and figure 2 As shown in (d), it can be seen that the particle size of Mn-MOF-74 microspheres is 2...

Embodiment 2

[0051] Example 2, Manganese metal organic framework biocomposite material as an impedance detection mode establishment of immunosensor

[0052] Weigh 10 mg of manganese metal-organic framework (Mn-MOF-74) nanospheres, and use deionized water for 10-fold gradient dilution to prepare an aqueous solution with a concentration ranging from 10 ng / mL to 10 μg / mL, and add a hydrogen peroxide solution with a concentration of 100 μM, After centrifugation, take 10 μL of the supernatant solution and add it dropwise to the working electrode working area of ​​the electrochemical workstation to test the impedance signals of electrochemical sensors with different concentrations.

[0053] It can be seen from the test that the impedance signal range in the deionized aqueous solution of the manganese metal organic framework biocomposite material in the concentration range of 10ng / mL~10μg / mL is 1116~10493Ω (see Table 1), and the linear equation is Y=1378X-738.68, R 2 = 0.9797.

[0054] The abov...

Embodiment 3

[0057] Example 3. Application of manganese metal organic framework immunosensor in the detection of Listeria monocytogenes in water and milk

[0058] Schematic diagram of the electrochemical detection technology for Listeria monocytogenes in water and milk based on metal-organic framework biocomposites Figure 4 shown.

[0059] Set the concentration to 10 8 The pure culture of CFU / mL Listeria monocytogenes was added to water and milk samples, and then the added samples were diluted with a 10-fold gradient in water and milk samples to prepare a concentration range of 10 0 ~10 4 CFU / mL of bacterial test solution. 100 μg of immunomagnetic beads were added to each diluted sample, and after incubation on a shaker for 20 minutes (40 rpm, room temperature), the mixture was magnetically separated for 1 minute, and then washed with PBS buffer and PBST buffer (0.05% Tween-20 PBS buffer, V / V) washed 3 times. Add 100 μg of MnMOF biocomposite to the magnetically separated samples and ...

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Abstract

The invention discloses a manganese metal organic framework (MOF) biological composite material and application thereof in detection of food-borne pathogenic bacteria. The manganese MOF biological composite material is formed by carrying out covalent coupling on Mn-MOF-74 nanometer microspheres and a specific biological recognition material; and the particle size of the Mn-MOF-74 nanometer microspheres is 200 to 300 nm. The manganese MOF biological composite material disclosed by the invention is an impedance type electrochemical immunosensor, which is sensitive in electrochemical impedance signal response, stable in structure and capable of specifically recognizing a target pathogenic bacterium-listeria monocytogenes, wherein Mn-MOF-74 crystals are uniform in size, good in biocompatibility and easy to modify; the prepared manganese MOF biological composite material has specific labelling capability on target bacteria and is easily reduced by hydrogen peroxide to generate manganese ions with good conductivity; and a listeria monocytogenes electrochemical detection technology developed by using the biological composite material has the advantages of simple operation, low detection limit and efficient and rapid detection process.

Description

technical field [0001] The invention relates to a manganese metal organic framework biocomposite material and its application in the detection of food-borne pathogenic bacteria, belonging to the technical field of detection of food-borne pathogenic bacteria. Background technique [0002] As an advanced porous material, metal-organic framework (MOF) has many functional characteristics such as structural designability, ordered spatial conformation, adjustable frame size, high porosity, and large surface area. Selective adsorption / separation, novel catalysis, microelectronic materials, chemical / biological sensing and in vivo drug delivery. Due to the large particle size of MOF crystals, the sensitivity to acid / alkaline solution stability and the limitation of non-specific adsorption, there are few studies on MOF as electrochemical detection probes. The existing MOF electrochemical sensor research is mainly based on the classic MOF structure, that is, forming a composite materi...

Claims

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Application Information

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IPC IPC(8): C07K16/00C08G83/00C12N15/115G01N27/06G01N27/26G01N33/569
CPCC07K16/00C12N15/115C08G83/008G01N33/56916G01N33/56938G01N33/56911G01N27/06G01N27/26C12N2310/16
Inventor 江海洋王嗣涵沈建忠王战辉熊进城徐宇良张亮王梓乐贺爽
Owner CHINA AGRI UNIV
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