Application of wheat flavone-5-O-glucoside in regulating and controlling resistance of plants to weeds

A technology of ticlavone and glucose, applied in the field of application of ticlavone-5-O-glucoside in regulating plant resistance to weeds, can solve problems such as genetic mechanism and weed suppression mechanism are not very clear

Active Publication Date: 2021-09-14
ZHONGKENONGFU (BEIJING) BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]The flavonoids found in rice that can inhibit the growth of weeds (allelopathic effect) are mainly triflavones, and whether other flavonoids have allelopathy However, the genetic mechanism of rice allelopathy and the mechanism of weed suppression are not very clear

Method used

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  • Application of wheat flavone-5-O-glucoside in regulating and controlling resistance of plants to weeds
  • Application of wheat flavone-5-O-glucoside in regulating and controlling resistance of plants to weeds
  • Application of wheat flavone-5-O-glucoside in regulating and controlling resistance of plants to weeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The compound was purified Example 1,

[0056] Rice (Indica) after about 60 days (1) collecting the fresh leaves sowing, triturated after freeze drier powder. Adding 8 volumes of 75% ethanol, ultrasonic extraction three times, each time 20min.

[0057] (2) The extract was filtered and concentrated to alcohol-free flavored by a rotary evaporator, followed by addition of an equal volume of petroleum ether, methylene chloride, and extracted three times with n-butanol respectively.

[0058] (3) The obtained n-butanol (Biotage Isolera concentrated using a rotary evaporator in a low pressure flash with a phase preparative liquid chromatography TM Prime) on the sample using a wet coarse fraction. Silica gel column YMC * GEL C18 packing of spherical filler, the filler particle diameter of 50 m, a pore size of 12nm. In this embodiment the filler weight of 100g. Water mobile phase (containing 0.1% formic acid, expressed in% volume percentage), methanol. The mobile phase was methanol r...

Embodiment 2

[0065] Example 2, Os07g0503900 Clone

[0066] (1) (Oryza sativa L.ssp.japonica cv.Zhonghua11, ZH11) Genomic DNA extraction of the leaves of rice seedlings in flower 11.

[0067] (2) According to the existing sequence information Os07g0503900 site annotation rice (Rice Genome Annotation Project), with primers containing BamHI and HindШ of F1 / R1 to ZH11 was amplified genomic DNA sequences containing CDS Os07g0503900 gene enzyme sites.

[0068] Primer F1: 5'-ggatccATGGCTCCAGCGATGGCGAG-3 ';

[0069] Primer R1: 5'-aagcttCTATATGGATGACATGTGGGC-3 '.

[0070] (2) The gene fragment was ligated to pEASY-T 3 Carrier (TransGen Biotech, -T3Cloning Kit), to transform E. coli DH5α competent cells, using blue-white screening positive clones.

[0071] (3) The positive clones using primers F2 / R2 identified PCR, the amplified fragment was 310bp for the positive clones. The primers are as follows:

[0072] Primer F2: 5'-AGGACTTCATCTCCCGGTTCATGC-3 ';

[0073] Primer R2: 5'-TTCTTCATCACAGGCGTCGGCAAC...

Embodiment 3

[0076] Application Example 3, Os07g0503900 gene embodiments

[0077] A prokaryotic expression vector

[0078] (1) cut with the restriction enzymes BamHI and HindШ recombinant vector of Example 2 obtained pEASY-T 3 -Os07g0503900 complete digestion, while cut expression vector pMAL-c2X (Chinese overseas, VECT-570). System for the digestion: 5μg plasmid, 2.5μL 10 × digestion buffer, 2μL BamHI, 2μL HindШ, add ddH 2 O complement reaction system to 50μL. Digestion reaction conditions are: enzyme 37 ℃ 4 hours.

[0079] (2) digested products by agarose gel electrophoresis to separate and recover a size of about 1.5Kb fragment containing Os07g0503900, and approximately 6.6Kb pMAL-c2X vector fragment were dissolved in 30μL ddH 2 O.

[0080] (3) The step (2) were obtained gene fragment with a vector backbone fragment ligation reaction. Ligation reaction is: 1μL 10 × ligase buffer, 0.5μL T 4DNA ligase, 1μL PMAL-C2X vector fragment, 3 μl of gene fragment, DDH 2 O Supplement the reaction system...

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PUM

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Abstract

The invention discloses an application of wheat flavone-5-O-glucoside in regulating and controlling the resistance of plants to weeds. The invention provides any one of the following applications of wheat flavone-5-O-glucoside: regulating and controlling plant allelopathy; regulating and controlling the weed resistance of plants; and inhibiting the weed growth. Experiments prove that the wheat flavone-5-O-glucoside can regulate the allelopathy of plants and can be used for inhibiting the growth of weeds. The wheat flavone-5-O-glucoside has important significance for exploring the plant allelopathy of the flavonoid compounds and developing environment-friendly green pesticides.

Description

Technical field [0001] The present invention relates to the field of biotechnology, particularly to the use of tricin -5-O- glucoside in the regulation of plant resistant weeds. Background technique [0002] Flavonoids are a widely distributed class of natural plant metabolites containing C6-C3-C6 basic skeleton. The degree of oxidation of the central three-carbon chain, and constitutes an annular ring connecting position B- (2- or 3-position), etc., it can be mainly divided into natural flavonoids flavonoids, flavonols, flavanone class, dihydro flavonols, anthocyanidins, isoflavones and so on. Most of flavonoids in plants as glycosides in combination with a sugar, also part of the present in free form (aglycone). Flavonoids have a strong anti-inflammatory and anti-cancer effect, have great potential applications in the treatment of cardiovascular disease, coronary heart disease, cancer and other diseases. In addition, flavonoids play an important role in plant growth and develop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01M21/04A01C1/00A01G7/00A01H6/46A01P21/00A01P13/00
CPCA01N43/16A01M21/043A01C1/00A01G7/00A01H6/4636
Inventor 漆小泉马爱民宋波
Owner ZHONGKENONGFU (BEIJING) BIOTECHNOLOGY CO LTD
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