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Method for improving quality of fermented cordyceps sinensis mycelia

A technology for fermenting Cordyceps sinensis and mycelium, applied in the field of Cordyceps sinensis, can solve the problems of low content of active ingredients and low nutritional value, etc.

Pending Publication Date: 2021-09-14
青海珠峰冬虫夏草原料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the pharmacological effects of artificially fermented Cordyceps and natural Cordyceps are similar, due to the difference in growth environment and growth cycle, the active ingredient content of artificially synthesized Cordyceps is still lower than that of natural Cordyceps, and the nutritional value is naturally lower than that of natural Cordyceps

Method used

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  • Method for improving quality of fermented cordyceps sinensis mycelia
  • Method for improving quality of fermented cordyceps sinensis mycelia
  • Method for improving quality of fermented cordyceps sinensis mycelia

Examples

Experimental program
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Effect test

preparation example 1

[0040] Preparation of brown algae liquid

[0041] Weigh 1Kg of fresh brown algae, wash them successively with ethanol and distilled water, cut them into pieces to obtain brown algae grains, add 3L of distilled water, soak the brown algae grains at room temperature for 3 hours, and alkalinize the obtained soaking solution with 1mg / mL sodium carbonate to pH 10, and stir for 5min , filtered to obtain the brown algae liquid, and then neutralized with 1:1 acetic acid to a pH of 6 to obtain a neutral brown algae liquid for use.

preparation example 2

[0043] Enzymolyzed Corn Flour and Silkworm Chrysalis Flour

[0044] Crush corn and silkworm chrysalis separately, sieve through a 40-mesh sieve, mix, and mix ingredients according to the specified ratio (corn flour: silkworm chrysalis powder = 2:3) and add them to the liquefaction tank. The total dosage is 2.6 tons, and water is added to make 6.5% For the liquefied material, add 0.25% calcium carbonate relative to the substrate, adjust the pH of the solution to 8.5, add 0.45% alkaline protease relative to the substrate, raise the temperature to 55°C, and perform proteolysis for 3.5 hours ;After the enzymolysis time is reached, adjust the pH of the solution to 5.5, add 0.40% α-amylase, the enzymolysis temperature is 90°C, and the enzymolysis time is 3.0h; then cool down to 55°C, adjust the pH value to 4.5, and add 0.30% α-amylase Glucoamylase, enzymatic hydrolysis time 1.5h, and then filtered to obtain a clear and transparent feed solution.

Embodiment 1

[0047] Select Chinese hairy sporium (Cs-C-Q80) as strain to carry out fermentation culture, carry out fermentation culture successively according to the following fermentation process flow:

[0048] S1. Slant culture: under aseptic conditions, firstly inoculate Mortierella sinensis on the slant medium, and culture at a constant temperature of 16-20°C for 5-7 days;

[0049] S2. Shake flask culture: put all the strains cultured in step S1 into the shake flask culture medium, culture at a constant temperature of 16-20°C, shaker speed 220-260r / min, and culture in the dark for 5-7 days;

[0050] S3, seed expansion cultivation: then insert the strains cultivated in step S2 into the seed expansion medium according to the inoculum amount of 5.0-10.0%, cultivate at a constant temperature of 16-20°C, the tank pressure is 0.05-0.10Mpa, and the ventilation volume is 1: (1.0~1.6)(V / V), cultivated in the dark for 6~9 days;

[0051] S4, fermentation culture: then insert the cultured bacteri...

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Abstract

The invention relates to the field of cordyceps sinensis, and particularly discloses a method for improving the quality of fermented cordyceps sinensis mycelia, which comprises the following steps: S1, slant culture, S2, shake-flask culture, S3, seed amplification culture, S4, fermentation culture, and S5, fermentation zone reculture. Components such as vitamins, jerusalem artichoke, folic acid, nucleoside precursors, brown algae liquid, cicada pollen and vegetable oil are added on the basis of an original fermentation culture medium; in addition, corn flour and silkworm chrysalis meal in the culture medium are subjected to enzymolysis in advance through a hydrolysis method; therefore, the content of nucleoside substances, D-mannitol, polysaccharide, fatty acid, ergosterol, gamma-aminobutyric acid and other active ingredients in fermented Hirsutella sinensis mycelia is increased; and the drug effect of artificially fermented cordyceps sinensis is enhanced.

Description

technical field [0001] The invention relates to the field of Cordyceps sinensis, more specifically, it relates to a method for improving the quality of fermented Cordyceps sinensis mycelium. Background technique [0002] The wild Cordyceps sinensis unique to the Qinghai-Tibet Plateau is a traditional precious and rare medicinal material in my country. It is a complex of insects and fungi formed by the fungus of the genus Cordyceps of the Ergotaceae family and the larvae of the bat moth. Because Cordyceps sinensis has the characteristics of mild medicinal properties and tonic but not harsh, the demand for it in domestic and foreign markets, especially in Japan and Southeast Asia, is ever-growing. [0003] In recent years, due to the limited resources of Cordyceps sinensis and serious damage during the excavation process, the resources of Cordyceps sinensis have become increasingly scarce. Researchers have conducted long-term and in-depth research on Cordyceps sinensis, trying...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/14C12R1/645
CPCC12N1/38C12N1/14
Inventor 李俊林朱永卿夏敏贞甘发月刘应奎韩桂香张金珠董笑菲杨成香
Owner 青海珠峰冬虫夏草原料有限公司
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