Preparation method and application of tea nanodots

A technology of nano-dots and tea leaves, applied in nano-technology, nano-medicine, nano-technology, etc., to achieve high extraction temperature, solve the problem of bacterial drug resistance, and enhance the stability

Active Publication Date: 2021-09-28
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Finally, how to reveal the antibacterial mechanism of these tea extracts through reasonable characterization methods, and find the component-target interaction relationship is also a huge challenge

Method used

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  • Preparation method and application of tea nanodots
  • Preparation method and application of tea nanodots
  • Preparation method and application of tea nanodots

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Experimental program
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Effect test

Embodiment 1

[0091] A method for preparing tea nano-dots, said method comprising the following steps:

[0092] 1) Pulverization: take the dry tea leaves of black tea, cut them into particles smaller than 0.5cm with scissors, and grind the tea particles into uniform tea powder with a mortar, the particle diameter of the tea powder is 0.8mm;

[0093]2) Extraction: 5g of the tea powder is mixed with 75g of water, placed in the polytetrafluoroethylene liner of the autoclave for hydrothermal reaction, sealed and placed in the polytetrafluoroethylene liner of the autoclave In the muffle furnace, raise the temperature of the muffle furnace to 80°C at a rate of 2°C / min, and keep it for 6 hours. The reaction pressure in the polytetrafluoroethylene liner of the high-pressure reactor is 1.6 standard atmospheric pressure. At room temperature, the crude extract was obtained;

[0094] 3) Separation: filter the crude extract with a filter cloth (150 mesh) to remove solids with a particle size greater th...

Embodiment 2

[0126] Taking Black TNDs as the research object, the construction of Black TNDs and the molecular dynamics (MD) simulation of the transmembrane process of Black TNDs are carried out, including the following parts:

[0127] 1. Obtain the top three Black TNDs models through molecular docking, and then evaluate their stability through MD simulation (100ns)

[0128] Construction of Black TNDs: Construction of Black TNDs by all-atom molecular dynamics (MD) simulations. First, construct the three-dimensional structures of eight molecules (CA, GC, CG, GCG, EC, EGC, ECG and EGCG), and use AutodockTools to assign them AD atom types respectively, then hydrogenate, charge, and finally save as ". pdb" type of file. Using the HEX8.0 program to sequentially dock the eight molecules constructed, the corresponding lowest energy modes among the eight molecules were calculated and selected as the input file for the next molecular docking. Then, the possible interaction modes and structural mo...

Embodiment 3

[0137] MRSA proteomic analysis of Black TNDs treatment, specifically including the following parts:

[0138] 1. Tandem mass tagging (TMT) technology for quantitative proteomics analysis: collect untreated and BlackTNDs-treated MRSA bacteria as bacterial samples for proteomic analysis, wash the bacterial samples 3 times, then add SDT lysate, and sonicate Treatment accelerated cell lysis, then boiled for 15 minutes, centrifuged at 14,000g for 40 minutes at 4°C, and the corresponding supernatant was quantified with a BCA protein assay kit (Bio-Rad, USA). The samples should be stored at -80°C before use. °C conditions. Next, protein samples were separated and visualized by SDS-PAGE. The filter-assisted sample preparation (FASP) method is performed during the enzymatic digestion of proteins. Additionally, the peptide mixture of each sample was labeled using TMT 6 / 10plex Isobaric Labeling Reagent (Thermo), and the TMT-labeled samples were fractionated using the Pierce High pH Reve...

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Abstract

The invention discloses a preparation method and antibacterial application of tea nanodots; and the method comprises the following steps of: crushing, extracting, separating, purifying, concentrating, drying and the like to obtain the tea nanodots (TNDs) with the particle size of 3 nm, which are assembled by at least six or more catechins through hydrogen bonds and pi-pi conjugates. The antibacterial activity and stability of the TNDs are superior to those of corresponding monomer small molecules and a mixture of the monomer small molecules; and researches on a mouse skin MRSA wound infection model and an MRSA infection pneumonia model find that: compared with clinical antibiotic vancomycin, the TNDs have a better curative effect. Due to the dual antibacterial mechanisms of physical transmembrane and targeted bacterial amino acid metabolism of the tea nanodots, the drug resistance of bacteria to the tea nanodots is not easy to generate; and meanwhile, the TNDs have good biocompatibility and in-vivo safety and have a certain clinical application prospect.

Description

technical field [0001] The invention belongs to the technical field of extracting functional components of natural medicines, and in particular relates to a method for preparing tea nano-dots and an application of the tea nano-dots prepared by the tea nano-dots preparation method. Background technique [0002] In China, tea has a history of more than 4,500 years. It is believed that tea was first used as a traditional Chinese medicinal material. China is the largest tea producer and consumer in the world, with rich tea tree resources and tea products. A large number of studies have shown that the most important composition and main active ingredient in tea is tea polyphenols, which belong to polyphenols and are a mixture of polyhydric phenol derivatives extracted from tea leaves. It is the main component, accounting for 60%-80% of the total phenol content, mainly composed of catechin ((+)-Catechin, CA), epicatechin ((-)-Epicatechin, EC), catechin gallate ((+)-Catechin galla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/82A61P31/04B82Y5/00B82Y40/00
CPCA61K36/82A61P31/04B82Y5/00B82Y40/00A61K2236/331A61K2236/15A61K2236/53A61K2236/51Y02P20/54
Inventor 吴水林李浚刘想梅崔振铎杨贤金
Owner TIANJIN UNIV
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