Application of mass spectrometry to detect the level of psm-e molecules in urine in the preparation of products for early diagnosis of prostate cancer

A technology for prostate cancer and urine, applied in the field of clinical examination and diagnosis, achieves the effects of wide application, convenient sampling and simple operation
CN113447654BActive Publication Date: 2022-03-22广州济士源生物技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
广州济士源生物技术有限公司
Publication Date
2022-03-22

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Abstract

The invention discloses the application of mass spectrometry technology to detect the PSM-E molecular level in urine in the preparation of products for early diagnosis of prostate cancer. The urine protein sample of the present invention is directly introduced into a liquid chromatography-electrospray ionization-tandem mass spectrometer for measurement after dilution, which can quickly and accurately detect the content level of PSM-E in urine; the fully synthesized PSM-E characteristic peptide standard is adopted The product is used as an internal standard to quantify the analyte, reducing the error caused in the sample pretreatment process; the liquid chromatography tandem mass spectrometry better improves the selectivity and accuracy of the method; the selection and optimization of the chromatographic column and elution conditions improve the The chromatographic separation process shortens the time of chromatographic analysis. The present invention can realize quantitative detection of PSM-E in urine specimens, has simple operation and convenient application, and clarifies that the content of PSM-E in urine can be used as a specific biomarker for prostate cancer detection, which is an early detection method for prostate cancer. Screening provides an efficient and feasible technical method.
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Description

technical field

[0001] The invention relates to the technical field of clinical inspection and diagnosis, in particular to the application of mass spectrometry technology to detect the level of PSM-E molecules in urine in preparing a product for early diagnosis of prostate cancer. Background technique

[0002] According to the "2020 National Cancer Center Annual Work Report", the incidence of prostate cancer in my country ranks ninth (total population), and the mortality rate ranks eighth (total population). The incidence of prostate cancer in my country is rising rapidly, and the age of onset is gradually younger. In addition, 60% to 70% of prostate cancer patients are locally advanced or advanced when they are first diagnosed. At present, there is a lack of accurate and convenient prostate cancer screening technology at home and abroad. Prostate specific membrane antigen (PSMA) is a transmembrane glycoprotein with the function of hydrolyzing folic acid and the neurotrans...

Claims

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