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Application of liriodendrin in medicine for treating radiation enteritis

A technology of radiation enteritis and lirioside, which is applied in the field of plant components in the treatment of diseases, can solve problems such as limited effects, and achieve the effect of broad clinical application prospects

Pending Publication Date: 2021-10-01
核工业四一六医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the prevention and treatment of acute radiation enteritis, the current diagnosis and treatment have not yet reached a consensus, and several aspects of prevention and treatment are needed. Only continuous improvement of radiotherapy techniques and methods can achieve the least harm to patients. The main treatment is intestinal flora Transplantation, drug protection, anti-inflammatory and antipyretic drugs, hemostatic drugs, mucosal protective agents, and some biological cell therapy, etc., but the effect achieved is limited

Method used

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  • Application of liriodendrin in medicine for treating radiation enteritis
  • Application of liriodendrin in medicine for treating radiation enteritis
  • Application of liriodendrin in medicine for treating radiation enteritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Establishment of radiation enteritis model

[0033] After the female C57BL / 6J mice were anesthetized, they were irradiated locally with Co60 gamma rays at 8Gy, using a GWXJ80 co-60 radiotherapy apparatus with an output dose of 39.22Gy / min to establish a radiation enteritis model (see attached figure 1 shown). The mice were randomly divided into a blank control group (control group), a model group (IR group), and a drug treatment group (IR+LD group, turioside concentration 100 mg / Kg), with 6 mice in each group. The mice in the IR+LD group were intragastrically administered with corresponding doses of turioside 3 days before irradiation and continued until the end of the experiment, and the control group and IR group were given equal volumes of distilled water. The mice in each group were sacrificed after 7 days. Table 1 below shows the reagents used in this example and their sources.

[0034] Table 1 Reagents required for model building

[0035] Reagent nam...

experiment example 1

[0038] H&E staining to evaluate the grade of enteritis tissue injury

[0039]After the treatment, the mice were sacrificed by cervical dislocation, the abdomen was opened to expose the ileocecal part, the proximal ileum was removed, and the intestinal secretions were rinsed with pre-cooled normal saline. About 0.5 cm of the ileum was taken from the ileocecal and fixed with 10% formalin solution for H&E staining. Reagents related to H&E staining are listed in Table 2 below.

[0040] The operation steps are:

[0041] 1. Making Paraffin Sections of Intestinal Tissue

[0042] Fix the ileal tissue with 4% paraformaldehyde solution for 24 hours; soak the fixed intestinal tissue in different concentrations of ethanol for 30 minutes; then use xylene and absolute ethanol (1:1) to soak for 2 hours; Xylene I and II respectively for 1.5 hours; then soaked in different paraffins, put the intestinal tissue soaked in wax into the tissue embedding box, pour the melted paraffin in the machi...

experiment example 2

[0054] Sphingolipidome analysis of mouse intestinal tissue (Cer, Cer1P, S1P)

[0055] Lirioside can alleviate intestinal pathological damage in mice, and detect the changes of Cer, Cer1P, and S1P through the sphingolipid pathway, suggesting that Lirioside plays an anti-inflammatory and anti-apoptotic role.

[0056] The operation steps are:

[0057] The collected ileal tissues were frozen in liquid nitrogen, and 5 samples from each group were randomly selected, a total of 15 samples from three groups were stored on dry ice and sent to Changzhou Zhongke Zhidian Biotechnology Co., Ltd. for sphingolipid group analysis. The company extracts lipids from the samples using a modified Bligh / Dyer extraction method (two extractions). Samples were reconstituted in isotope mix and all analyzes were performed using an ExionUPLC-QTRAP 6500Plus (Sciex) LC / MS in electrospray ionization (ESI) mode. Using Phenomenex Luna silica 3μm (inner diameter 150x2.0mm) chromatographic column, various pol...

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Abstract

The invention provides an application of liriodendrin in a medicine for treating radiation enteritis. The invention finds that the liriodendrin has anti-inflammatory and anti-apoptosis effects in radiation enteritis, and through nerve sphingolipid pathway detection, it is found that the liriodendrin can reduce the expression of Cer, Cer1P and S1P, which prompts that the liriodendrin has anti-inflammatory and anti-apoptosis effects on radiation enteritis, and the liriodendrin can inhibit the expression of proinflammatory cytokines (IL-6 and TNF-alpha) in the intestinal tract of a mouse with radiation enteritis. Besides, the liriodendrin can effectively inhibit expression of intestinal tissues p-NF-kB of mice suffering from radiation enteritis, and the liriodendrin can also reduce the number of neutrophils in the intestinal tissues, which shows that the liriodendrin has an anti-inflammatory effect in radiation enteritis. A theoretical basis is provided for deep research on pathogenesis of radiation enteritis and development of new drugs, so that a scientific basis is provided for clinical treatment of radiation enteritis by using liriodendrin, and a more beneficial treatment scheme can be provided for patients with radiation enteritis.

Description

technical field [0001] The invention relates to the technical field of application of plant ingredients in disease treatment, in particular to the application of turioside in medicine for treating radiation enteritis. Background technique [0002] Radiation Enteritis (RE) is simply defined as the process of intestinal inflammation due to exposure to radiant energy. Patients receiving pelvic or abdominal radiation therapy may experience acute and / or chronic side effects due to changes in the gastrointestinal tract. Ionizing radiation injury to the gastrointestinal tract is a common side effect of radiation therapy and a significant proportion of patients suffer acute or chronic gastrointestinal bowel symptoms. External beam radiation therapy is a common treatment modality for a variety of cancers, with approximately 70% of cancer patients receiving radiation therapy during the course of their disease and radiation therapy accounting for 25% of all cancer treatments. [0003...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61K36/185A61P1/00A61P29/00
CPCA61K31/7048A61K36/185A61P1/00A61P29/00
Inventor 刘斌蒋晶晶郑新李佳俊黎熊江洋
Owner 核工业四一六医院
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