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Nano fluorescent probe as well as preparation method and application thereof

A technology of nano-fluorescent probes and fluorescence intensity, which is applied in the field of nano-fluorescent probes and its preparation, can solve the problems of insufficient detection accuracy and precision, and achieve the effects of no toxic side effects, good biocompatibility, and high precision

Pending Publication Date: 2021-10-22
SUZHOU GUOKE MEDICAL TECH DEV CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods either require expensive instrument support, or the detection accuracy and precision are not enough

Method used

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  • Nano fluorescent probe as well as preparation method and application thereof
  • Nano fluorescent probe as well as preparation method and application thereof
  • Nano fluorescent probe as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A kind of nano fluorescent probe of the present embodiment, it is prepared by the following method:

[0047] 1) 1000mg trisodium citrate, 1000mg FeCl 3 Dissolve together with 2mL ethylenediamine in 30mL ethylene glycol, ultrasonicate and stir vigorously until the solution is clear;

[0048] 2) Transfer the mixed solution obtained in step 1) to a 100mL polytetrafluoroethylene-lined reactor, and react in an oven at 200°C for 8 hours;

[0049] 3) After the reaction, cool to room temperature, centrifuge the reacted solution at 10,000 rpm for 10 min to remove large particles, and then use a rotary evaporator to remove ethylene glycol;

[0050] 4) Dialyze the solution obtained in step 3) with a dialysis bag with a molecular weight cutoff of 1000Da for 24 hours to remove unreacted raw material small molecules, then collect the solution in the dialysis bag and freeze-dry to obtain purified carbon quantum dots, which is the Nano fluorescent probes.

Embodiment 2

[0052] Carry out performance test to the carbon quantum dot that embodiment 1 makes

[0053] refer to figure 1, is the transmission electron microscope photo of the carbon quantum dots prepared in Example 1. As can be seen from the figure, the carbon dots are spherical in shape, with a relatively uniform particle size, a single size of about 3-5nm, and a dispersibility in water very good. The super-resolution TEM photo in the upper left corner shows that the carbon dots have an obvious lattice structure with a lattice spacing of 0.21nm.

[0054] refer to figure 2 , is the ultraviolet-visible absorption spectrogram and the fluorescence spectrogram of the carbon quantum dot that embodiment 1 makes, wherein comprise the absorption spectrogram (curve 1: Fe, N-CDs) of carbon quantum dot, carbon quantum dot and hemin The absorption spectrum of the mixture solution (curve 3: Fe, N-CDs+hematin), the absorption spectrum of hemin (curve 2: Hematin), the fluorescence excitation spect...

Embodiment 3

[0063] This example provides an application of the carbon quantum dots prepared in Example 1 for the detection of hematin concentration.

[0064] The method that the nanometer fluorescent probe is used for the detection of hematin concentration comprises the following steps:

[0065] 1) Formulate the standard curve: Weigh dry carbon quantum dots, add ultrapure water, configure a carbon quantum dot aqueous solution with a concentration of 5 μg / mL, and adjust the pH value of the carbon quantum dot aqueous solution to 10.0 with 0.1mol / L NaOH solution , the equal volume is divided into 15 parts, and under the excitation condition of 365nm, the fluorescence intensity of each carbon quantum dot aqueous solution at 450nm is tested, which is recorded as the original fluorescence intensity;

[0066] Add 0, 1, 2, 3, 4, 5, 7, 9, 11, 13, 15, 18, 21, 24, 27 μM of hematin to 15 parts of carbon quantum dot aqueous solution, so as to obtain different hematin The carbon quantum dot aqueous so...

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Abstract

The invention discloses a nano fluorescent probe as well as a preparation method and application thereof. The nano fluorescent probe is prepared by the following method: 1) dissolving trisodium citrate, FeCl3 and ethylenediamine in ethylene glycol together, and performing ultrasonic stirring until the solution is clear; 2) transferring the mixed solution into a reaction kettle with a polytetrafluoroethylene lining, and reacting under a heating condition; 3) after the reaction is finished, cooling to room temperature, centrifuging, and then removing ethylene glycol by using a rotary evaporator; and 4) dialyzing the solution by using a dialysis bag, collecting the solution in the dialysis bag, and freeze-drying to obtain a purified carbon quantum dot, namely the nano fluorescent probe. The carbon quantum dot disclosed by the invention has the characteristics of simple preparation method, good water solubility, good biocompatibility, no toxic or side effect and the like, and large-scale production can be realized; according to the hemin detection method provided by the invention, the concentration of hemin can be effectively detected through the change of the fluorescence intensity of the carbon quantum dots; and the method has the advantages of simplicity and convenience in operation, high accuracy, good selectivity and the like.

Description

technical field [0001] The invention relates to the field of nanometer materials, in particular to a nanometer fluorescent probe, its preparation method and application. Background technique [0002] Hematin (Hematin), also known as hydroxyheme or ferric porphyrin, is a complex of protoporphyrin and ferric iron. Produced by oxidation of heme, which is an important component of hemoglobin. Excess heme is harmful to human health because its metabolite bilirubin is toxic to the brain and nervous system. In addition, related studies have shown that high levels of hemoglobin may lead to various diseases, including immune system disorders, vascular diseases and polycythemia vera. Therefore, it is of great significance to realize the sensitive and rapid detection of hemoglobin. [0003] At present, the existing detection methods of hemin include electrochemical method, mass spectrometry, colorimetric method, high performance liquid chromatography and so on. These methods either...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65G01N21/64
CPCC09K11/65G01N21/6428G01N21/6456G01N2021/6432
Inventor 李力曹磊常智敏董文飞梅茜葛明锋从瑛哥
Owner SUZHOU GUOKE MEDICAL TECH DEV CO LTD
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