Photonic crystal molecularly imprinted hydrogel sensor based on water phase as well as preparation method and detection method of photonic crystal molecularly imprinted hydrogel sensor

A technology of photonic crystals and molecular imprinting, which is applied in the direction of material analysis through optical means, material analysis through observation of the impact on chemical indicators, instruments, etc., can solve the problem that it is difficult to be applied to the detection of protein molecules, and the preparation method has not been reported. , prone to denaturation and other issues, to achieve good selectivity and sensitivity, easy preparation, and maintain activity

Inactive Publication Date: 2021-11-05
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although there are various preparation methods and detection targets of MIPH, the traditional method of preparing MIPH in an organic phase is difficult to be applied to the detection of specific protein molecules due to the specific thr

Method used

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  • Photonic crystal molecularly imprinted hydrogel sensor based on water phase as well as preparation method and detection method of photonic crystal molecularly imprinted hydrogel sensor
  • Photonic crystal molecularly imprinted hydrogel sensor based on water phase as well as preparation method and detection method of photonic crystal molecularly imprinted hydrogel sensor
  • Photonic crystal molecularly imprinted hydrogel sensor based on water phase as well as preparation method and detection method of photonic crystal molecularly imprinted hydrogel sensor

Examples

Experimental program
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Example Embodiment

[0039] Example 1

[0040]A aqueous phase-based photonic crystal molecular blot polymer hydrogel film, characterized in that the hydrogel has a photonic crystal structure of an antibolite, and is a highly orderly porous material having a photon. And band gaps, large specific surface area, can produce Prague diffraction in light irradiation of a particular wavelength.

[0041] The photonic crystal molecular imprinting hydrogel film according to claim 1, wherein the template of the antisine stone structure is obtained by a silica microsphere having a size of 283 nm by vertical self-assembly method, and the microspheres are tight. , Structural rules. The hydrogel is polycolized and wrapped in a template surface, and the silica microspheres are removed by hydrofluoric acid to obtain a hydrogel having a dehydrating protein photonic crystal structure.

[0042] The photonic crystal molecule imprinting hydrogel film according to claim 1, wherein the hydrogel film is composed of a crosslink...

Example Embodiment

[0045] Example 2

[0046] 1) Use silicate, ammonia water, water and ethanol to synthesize a silica microsphere having a diameter of 283 nm, washed with ethanol and centrifuge and dried, and the dry product is formulated into a mass fraction of 2% silica-ethanol. Dispersions;

[0047] 2) Crack the glass sheet into 1 mm × 8 mm × 76 mm and 1 mm × 10 mm × 30 mm, respectively, respectively as a slide and a tablet. Wash with deionized water, soaked in the eating fish solution (98% H2SO4-30% H2O2, 7: 3, v / v), it was cleaned with deionized water and ethanol, and nitrogen was used.

[0048] 3) Insert the slide vertically into the dispersion, using a vertical self-assembly method in the glass surface of the protein stone structure in the glass surface; the self-assembly temperature is 35 ° C, the time is 72 h;

[0049] 4) The 23 mg of target protein was dissolved in 1.0 ml of phosphate buffer (0.2 mol / L, pH = 6.4), 15 mGN, N'-methylene biscrylamide and 174 mg acrylamide, mixed for 5 min...

Example Embodiment

[0054] Example 3

[0055] The photonic crystal molecular blot hydrogel sensor was prepared based on Example 2:

[0056] 1) Immersed hydrogel in a standard solution containing different concentration gradients. After incubating for 20 min at room temperature, the Prague diffraction peak wavelength of the hydrogel was measured using an ultraviolet spectrophotometer, and the amount of change and concentration of the wavelength was described.

[0057] 2) Immerse hydrogel in a 500 μg / ml of lysozyme standard solution, using an ultraviolet spectrophotometer to measure the Prague diffraction peak wavelength of the primary hydrogel every 2.5 minutes until the wavelength does not change, and the wavelength and time ;

[0058] 3) Determination of the selectivity of the photon crystal molecule imprinting hydrogel film was used to determine the selectivity of the photonic crystal molecule imprint.

[0059] Append image 3 As shown, the wavelength of the Prague diffraction peak is well formed ...

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Abstract

The invention researches a photonic crystal molecularly imprinted hydrogel sensor based on a water phase as well as a preparation method and a detection method of the photonic crystal molecularly imprinted hydrogel sensor, and belongs to the field of molecularly imprinted sensing. According to the invention, Lysozyme (Lyz) is selected as a research object, a molecular imprinting technology is combined with photonic crystals, and the photonic crystal molecularly imprinted hydrogel sensor is prepared in a water phase in a simple mode through a novel imprinting method and a novel imprinting system. The preparation method comprises the steps of preparing monodisperse silicon dioxide microspheres by adopting an improved method, then preparing a photonic crystal template by adopting a vertical self-assembly method, polymerizing a polymerization solution on the surface of the template to form a film, removing the photonic crystal template by using hydrofluoric acid, and finally obtaining the photonic crystal molecularly imprinted hydrogel sensor with the inverse opal structure by using the template. The hydrogel sensor has good selectivity and sensitivity, and can be successfully used for detecting lysozyme in an actual sample.

Description

technical field [0001] The invention belongs to the field of molecular imprinting sensing, and in particular relates to an aqueous phase-based photonic crystal molecular imprinting hydrogel sensor and a preparation method and a detection method thereof. Background technique [0002] Lysozyme (Lysozyme, Lyz) is a macromolecular protein with a molecular weight of about 14-15kDa. As an alkaline enzyme, it can hydrolyze mucopolysaccharides in bacteria and then lyse the bacteria, which has a certain antibacterial effect. Lysozyme widely exists in microorganisms, animal and plant tissues, egg whites of birds and poultry, tears, blood and milk of mammals, and milk contains about 13 μg lysozyme per 100 mL. Due to its special antibacterial properties, it can be used as a good additive in baby food and beverages. At present, there has been a practice of increasing the content of lysozyme in milk by inoculating certain specific antigens to cows to improve the quality of milk. [000...

Claims

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Application Information

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IPC IPC(8): G01N21/47G01N21/78C08J5/18C08J9/28C08F220/56C08F222/38C08L33/26
CPCG01N21/4788G01N21/783C08J5/18C08J9/28C08F220/56C08J2333/26C08F222/385
Inventor 罗爱芹刘宗坤侯慧鹏梁阿新刘毅权许立霖王微
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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