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Kanamycin grafted cellulose-based antibacterial material and preparation method thereof

A cellulose-based, antibacterial material technology, applied in the direction of antibacterial drugs, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem of low antibacterial activity and limit the application of chitosan/cellulose materials and other problems, to achieve the effect of simple reaction, reducing water pollution problems, and low cytotoxicity

Pending Publication Date: 2021-12-21
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The low antibacterial activity of chitosan against pathogens also limits the application of chitosan / cellulose materials

Method used

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  • Kanamycin grafted cellulose-based antibacterial material and preparation method thereof
  • Kanamycin grafted cellulose-based antibacterial material and preparation method thereof
  • Kanamycin grafted cellulose-based antibacterial material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Disperse 5 g of α-cellulose and 0.058 g of kanamycin sulfate in 10 mL of water, add 5 μL of 0.1 M hydrochloric acid dropwise, and stir for 30 minutes; under dark conditions, add 0.05 g of glutaraldehyde dropwise, and stir at room temperature for 2 h; filter, Collect the particles, remove incompletely reacted kanamycin sulfate with a large amount of pure water, and dry in vacuum at 60° C. for 5 hours to obtain a cellulose-based antibacterial material grafted with kanamycin sulfate.

[0039] Detect the inhibitory effect of the cellulose-based antibacterial material of this embodiment on Escherichia coli (Gram-negative bacteria): Inoculate Escherichia coli into a liquid medium (LB medium), cultivate at 37°C, until the bacterial liquid OD 600 =1.39, which is the original bacterial liquid. Take 1mL of the original bacterial liquid, add 50mg of the cellulose-based antibacterial material of this example or α-cellulose, shake at 37°C for 3h, smear the plate, and detect the numb...

Embodiment 2

[0041] Disperse 5g of cotton cellulose and 5.8g of kanamycin hydrochloride in 50mL of water, add dropwise 2.5mL of 0.1M acetic acid aqueous solution, and stir for 30 minutes; under dark conditions, add dropwise 5g of adipaldehyde, and stir at room temperature for 6h; Filter, collect particles, remove incompletely reacted kanamycin hydrochloride with a large amount of pure water, and vacuum dry at 40°C for 10 hours to obtain a cellulose-based antibacterial material grafted with kanamycin hydrochloride, with a BSA adsorption capacity of 162.5 mg / g.

[0042]Detect the inhibitory effect of the cellulose-based antibacterial material of this embodiment on Staphylococcus aureus (Gram-positive bacteria): inoculate Staphylococcus aureus into a liquid medium (LB medium), cultivate at 37 ° C, until the bacterial liquid OD 600 =1.18, which is the original bacterial liquid. Take 1mL of the original bacterial liquid, add 50mg of the cellulose-based antibacterial material of this example or ...

Embodiment 3

[0046] Disperse 5g of pulp (without water, cellulose content > 85%) and 2g of kanamycin sulfate in 20mL of water, add dropwise 500μL of 0.5M phosphoric acid aqueous solution, and stir for 1 hour; Dialdehyde, stirring at room temperature for 12 hours; filtering, collecting particles, removing incompletely reacted kanamycin sulfate with a large amount of pure water, and vacuum drying at room temperature for 24 hours to obtain a cellulose-based antibacterial material grafted with kanamycin sulfate.

[0047] Detect the inhibitory effect of the cellulose-based antibacterial material of this embodiment on Salmonella (Gram-negative bacteria): inoculate Salmonella into a liquid medium (LB medium), cultivate at 37 ° C, until the solution OD 600 = 2.19. Take 1mL of the original bacterial liquid, add 50mg of the cellulose-based antibacterial material or paper pulp of this example, shake at 37°C for 3h, smear the plate, and detect the number of Salmonella growing on the nutrient agar plat...

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Abstract

The invention discloses a kanamycin grafted cellulose-based antibacterial material. The kanamycin grafted cellulose-based antibacterial material is prepared by taking cellulose as a main body material, acid as a catalyst and alkyl dialdehyde as a cross-linking agent and grafting antibiotics through a chemical method. According to the kanamycin grafted cellulose-based antibacterial material, the cellulose does not need to be pre-modified and is directly grafted with kanamycin through glutaraldehyde; linking sites are hydroxyl of the cellulose and hydroxyl of the kanamycin and belong to an aldol reaction; the reaction is simpler and more rapid. The cellulose-based antibacterial material disclosed by the invention is not dissolved into water and a conventional organic solvent, so that the problem of water pollution caused by the fact that the antibiotics are easily dissolved is effectively reduced. The cellulose-based antibacterial material disclosed by the invention has a remarkable inhibition effect on gram-positive bacteria and gram-negative bacteria, is a stable, efficient, broad-spectrum and edible antibacterial agent and has extremely low cytotoxicity. The cellulose-based antibacterial material disclosed by the invention has good gastric acid resistance and can be used as an oral antibacterial material; after the cellulose-based antibacterial material is used for treating gastric acid, intestinal bacteria are directly inhibited.

Description

technical field [0001] The invention belongs to the field of functional materials, and relates to a cellulose-based antibacterial material and a preparation method thereof, in particular to a graft-modified cellulose-based antibacterial material and a preparation method thereof. Background technique [0002] It is well known that routine medical procedures such as surgery, intensive care medicine and infection treatment cannot do without antibiotics. However, the abuse of antibiotics has led to the development of drug resistance of a large number of pathogenic bacteria, seriously endangering human life and health. Researchers predict that by 2050, antimicrobial resistance will kill more than 10 million people, surpassing the death rate from cancer. Therefore, it is of great market value to develop a new, efficient and safe antibacterial material that is different from traditional antibiotics. [0003] Chitosan-based antimicrobial agents (CAMs), especially chitosan micro- a...

Claims

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Application Information

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IPC IPC(8): A61K47/61A61K47/69A61K31/7036A61P31/04
CPCA61K47/61A61K47/6953A61K31/7036A61P31/04
Inventor 李彬李澧范宝超顾俊张帅锋张雪寒夏学峰周俊明祝昊丹汪伟王丹丹
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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