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Two-phase oil adjuvant foot-and-mouth disease vaccine stabilizer and application thereof

A foot-and-mouth disease vaccine and stabilizer technology, applied in the field of animal biological products, can solve the problems of reduced immune effect, undetectable antibodies, and demulsification, so as to reduce animal stress, ensure immunogenicity, and increase the proportion of water phase Effect

Pending Publication Date: 2022-01-07
CHINA ANIMAL HUSBANDRY IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the current production and application of foot-and-mouth disease vaccines, there are problems such as unstable immune effect, undetectable or low level of antibody production after immunization, or short immunization period. One of the important reasons for these problems is due to The oil-water emulsion of the existing biphasic oil adjuvant foot-and-mouth disease vaccine is a thermodynamically unstable system. After the commercially available oil adjuvant is emulsified, the vaccine is extremely easy to separate and even break the emulsion, and the integrity and immunogenicity of the effective antigen have not been fully guaranteed. Protect
At the same time, when the biphasic emulsion oil adjuvant vaccine is injected into the animal body, if the emulsion is quickly decomposed and destroyed by the body fluid, and the effective antigen of foot-and-mouth disease loses the protection of the emulsion, the antigen originally wrapped in the emulsion will enter the body fluid and be quickly decomposed, shortening the The time for antigen to stimulate the body, the effective antigen can not be fully presented by cells, the immune effect will be greatly reduced, leading to immune failure, the immune effect of the vaccine can not be fully reflected, these status quo are unfavorable to the development of the foot-and-mouth disease vaccine industry influences
At the same time, since the oil adjuvant component occupies a considerable proportion in the composition of the existing biphasic oil adjuvant foot-and-mouth disease vaccine, the occurrence of animal side effects is inevitable.

Method used

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  • Two-phase oil adjuvant foot-and-mouth disease vaccine stabilizer and application thereof
  • Two-phase oil adjuvant foot-and-mouth disease vaccine stabilizer and application thereof
  • Two-phase oil adjuvant foot-and-mouth disease vaccine stabilizer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0025] As a preferred embodiment of the stabilizer of the present invention, the acid comprises tartaric acid, citric acid, lactic acid, malic acid, maleic acid, sorbic acid, succinic acid, ricin alcohol, citric acid; the alcohol includes Alcohol, elevale alcohol, dodecyl alcohol (lauryl), tetrahydroganol (nargol), hexadecanol (palmitol), eaterseanol (stearol), duethanol (peanut alcohol) ), Twenty di alcohol (hull alcohol); the amine such as glutamine, asparagine, nicotinamide; the charge modifier comprises sodium chloride, potassium chloride, ammonium chloride, monoethanolamine chloride, Diethanolamine chloride, disodium hydrogen phosphate, sodium phosphate, sodium triphosphate, sodium stearate, magnesium stearate, gluconate, acetate, citrate; the polymer includes hydroxyethyl Cellulose, polyethylene glycol (PEG), glucan, acrylate, and copolymers thereof, polyveridate, polyvinyl alcohol, polyacrylic acid, and sodium salt, chitosan, natural glue, and modifiers such as Algic acid a...

Embodiment 1

[0033] Example 1, a stabilizer for a foot-and-mouth disease vaccine applied to a double photograph oil?

[0034] The stabilizer of the footheroid vaccine applied to the double-phase oil rejuperature of this embodiment consists of a substance of the following mass percentage:

[0035] Acid: 0.2% 酒 石 石, 0.1% of sorghum acid,

[0036] Alcohol: 0.3% decanol, dodecylol (lauryl) 0.2%,

[0037] Amine: 0.3% of glutamine,

[0038] Polymer: 1% of sodium carboxymethylcellulose, potassium alginate 0.5%,

[0039] Charge regulator: 0.5% of potassium chloride, 0.5% sodium octanoate,

[0040] Sugar: 2% lactose, 2% mannose, 5% of dimethyl-β-cyclodextrin,

[0041] Water for injection: 87.15%.

Embodiment 2

[0042] Example 2, a stabilizer of a foot-and-mouth hyperplate applied to a double photographic oil joiner

[0043] The stabilizer of the footheroid vaccine applied to the double-phase oil rejuperature of this embodiment consists of a substance of the following mass percentage:

[0044] Acid: 0.2% linoleic acid, 0.1% succinate,

[0045] Alcohol: Eateginol (stearol) 0.2%,

[0046] Amine: nicotinamide 0.3%, 5.1% of glutamine,

[0047] Polymer: 1%, polyethylene glycol (40) hydrogenated castor oil 1.5%,

[0048] Charge regulator: 0.5% of hydrogen phosphate, 0.04% podium phosphate, 0.1% of potassium chloride, 0.5% gluconate,

[0049] Sugar: 2% maltose, 2% fibrous diose, 3% of hydroxypropyl-β-cyclodextrin,

[0050] Water of injection: 88.46%,

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PUM

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Abstract

The invention discloses a two-phase oil adjuvant foot-and-mouth disease vaccine stabilizer and application thereof The two-phase oil adjuvant foot-and-mouth disease vaccine stabilizer is prepared from the following components in percentage by weight of 0.1 to 1 percent of acid, 0.1 to 2 percent of alcohol, 0.1 to 2 percent of amine, 0.1 to 10 percent of charge control agent, 0.1 to 15 percent of polymer, 0.1 to 20 percent of sugar and the balance of water. The stabilizer provided by the invention improves the preservation effect, immune efficacy and safety level of the two-phase oil adjuvant foot-and-mouth disease vaccine.

Description

Technical field [0001] The present invention belongs to the technical field of animal biological products, and specific to stabilizers and their applications thereof are related to double-phase oil joined foot-and-mouth disease vaccine. Background technique [0002] Foot-and-mouth disease is an acute, thermal, highly contactive infectious disease caused by the cow, pig, and sheep caused by foot and mouth disease virus, FMDV, which can reduce animal production performance, causing huge direct economy. Loss, was listed by OIE as the first place in 16 kinds of animal diseases. The foot-and-mouth disease virus belongs to small RNA virus, and there are seven serotypes of foot-and-mouth disease: A type, O type, C-type, SAT1 type, SAT2 type, SAT3 type, ASIA1, foot-and-mouth disease antigen immunogenic immunogenicity, antigen No crossover protection between various types. The prevention and control measures for foot-and-mouth disease are mainly immunized by immunization prevention, and e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K9/113A61K47/38A61K47/36A61K47/02A61K47/26A61K47/40A61K47/18A61K47/12A61K47/10A61K47/44A61K47/32A61K47/22A61K47/04A61K39/135A61P31/14
CPCA61K39/39A61K9/113A61K47/38A61K47/36A61K47/02A61K47/26A61K47/40A61K47/183A61K47/12A61K47/10A61K47/44A61K47/32A61K47/22A61K39/12A61P31/14A61K2039/5252A61K2039/552A61K2039/54C12N2770/32134
Inventor 潘京学严石黄书林潘春刚
Owner CHINA ANIMAL HUSBANDRY IND
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