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Improved compound microbial agent and application thereof in yield increase of angelica sinensis

A technology of compound microbial inoculants and microbial fertilizers, applied in the directions of microorganism-based methods, microorganisms, and chemicals used for biological control, can solve problems such as urea burning and ammonium salt poisoning that have not been considered, and achieve the goal of promoting the absorption of rich nutrients. The effect of collecting, promoting transformation and improving plant yield

Active Publication Date: 2022-01-18
甘肃绿能农业科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The inventor has studied the impact of Pseudomonas compound microbial bacterial agent on the physiology, biochemistry and quality of continuous cropping Angelica sinensis, and has applied for a national invention patent (a kind of Pseudomonas compound microbial bacterial agent and application in Angelica sinensis disease resistance, yield increase and quality improvement), but the problem of burning seedlings and ammonium salt poisoning caused by a large amount of urea and unreasonable use of farmers in the planting process has not been considered. Phosphorus can promote flowering. Therefore, Angelica planting related bacterial agents The development should also consider the problem of phosphorus solution

Method used

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  • Improved compound microbial agent and application thereof in yield increase of angelica sinensis
  • Improved compound microbial agent and application thereof in yield increase of angelica sinensis
  • Improved compound microbial agent and application thereof in yield increase of angelica sinensis

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Effect test

Embodiment 1

[0043] Example 1 Determination of microbial phosphorus solution, nitrogen fixation, and nitrification

[0044] 1. Qualitative detection of phosphorus dissolving ability

[0045] Inoculate different strains on NBRIP inorganic phosphorus solid medium, culture at a constant temperature of 28°C for 2-5 days, observe whether there is a phosphorus-dissolving circle and the size of the phosphorus-dissolving circle, and determine its phosphorus-dissolving effect on inorganic phosphorus according to the size of the phosphorus-dissolving circle effect.

[0046] 2. Quantitative detection of nitrogen fixation activity

[0047] Take 5ml of the purified bacterial solution and inoculate it in 50ml of sterilized Ashubei nitrogen-free medium, 200r min -1 , shake culture at 30°C for 72h, measure the turbidity OD 600 .

[0048] Determination of the nitrogenase activity of strains by acetylene reduction method: pipette 5ml of the above-mentioned fresh bacterial solution into a 20ml headspace ...

Embodiment 2

[0058] The impact of different treatments of embodiment 2 on the yield-related indicators of Angelica sinensis

[0059] After harvesting, the number of indeterminate roots was calculated by conventional methods, the diameter, length and body length of the reed head were measured with a vernier caliper, and the fresh weight of a single plant was weighed with an electronic scale. 300 plants were measured for each treatment, and the average value was calculated.

[0060] The results are shown in Table 2. Compared with CK, the number of adventitious roots, reed head diameter, reed head length, body length, and average fresh weight of Angelica sinensis after treatment with CBS4 single bacteria basically did not change. The number of adventitious roots, diameter of reed head, length of reed head, body length and average fresh weight per plant all increased. Compared with the CK group, after being treated with the compound bacterial agent (T1) of the present invention, the number of ...

Embodiment 3

[0064] Embodiment 3 angelica rhizosphere soil enzyme activity assay

[0065] According to the method described in the kit, the rhizosphere soil urease (S-UE), soil sucrase (S-SC), soil FDA hydrolase, soil β-glucosidase (S-β-GC), soil alkali Protease (S-ALPT), soil alkaline phosphatase activity (S-AKP / ALP), soil catalase (S-CAT) activity, each sample was repeated 3 times, and the average value was calculated.

[0066]The effects of different treatments on rhizosphere soil enzyme activities are shown in Table 3. Compared with the CK group, T1 and T2 compound bacterial agents (S2 and S3 periods) all increased the FDA hydrolase, soil invertase, soil-β-glucosidase, soil urease, and soil alkaline protease in the rhizosphere of Angelica sinensis. The enzyme activity decreased the activity of alkaline phosphatase; the results showed that the two compound bacterial agents could improve the microbial activity of rhizosphere soil, the transformation ability of organic matter, promote ca...

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Abstract

The invention belongs to the technical field of microorganisms and biology, and particularly relates to an improved compound microbial agent and application thereof in yield increase of angelica sinensis. The compound microbial agent is prepared by compounding pseudomonas fluorescens, pseudomonas alcaligenes, pseudomonas psychrophila and zoogloeosporium cladosporium, and the compound microbial agent not only retains the functional characteristics of the original microbial agent, but also improves the conversion, absorption and utilization of soil nutrients;in addition, the compound microbial agent can promote ammonium nitrogen in rhizosphere soil to be converted into nitrate nitrogen, the toxic effect of soil ammonium salt on plants is reduced, and the content of available nitrogen in the rhizosphere soil is increased; the activity of nitrate reductase in the plants can be enhanced, nitrate nitrogen in the plants is promoted to be converted into ammonium nitrogen, nitrogen absorption and enrichment of the plants are promoted, and the plant yield is increased.

Description

technical field [0001] The invention belongs to the field of microorganism and biotechnology, and in particular relates to an improved compound microbial bacterial agent and its application in increasing the production of angelica. Background technique [0002] Angelica sinensis is the dried root of Angelica sinensis (Oliv.) Diels, a plant of the Umbelliferae family. It is a traditional Chinese medicine and has been included in the catalog of homology of medicine and food. Gansu is the main producing area of ​​angelica, with a planting area of ​​400km2 in the whole province 2 , yields 1.2 x 10 5 kg, accounting for more than 80% of the total supply in the national market, and plays a decisive role in the national angelica market price. Angelica can be cultivated at an altitude of about 1500-3000m. It likes alpine and cool climate. It likes deep, loose, well-drained, fertile sandy loam rich in humus. It is not suitable for low-lying water accumulation or clay and barren sand...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/27A01N63/20A01P21/00C05G3/90C05G3/00C12R1/39C12R1/38C12R1/01
CPCC12N1/20A01N63/27A01N63/20C05G3/90C05G3/00Y02P60/21
Inventor 杨涛牛永艳王治业赵疆毛婷姚阳阳李鑫
Owner 甘肃绿能农业科技股份有限公司
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