Actinomycete strain SCAUT013 and application thereof

A technology of actinomycetes and strains, applied in the field of microorganisms, to achieve the effect of promoting nodulation and growth, and good application potential

Active Publication Date: 2022-02-08
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since A. glabra is a leguminous forage, previous studies mainly focused on the symbiotic relationship between rhizobia

Method used

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  • Actinomycete strain SCAUT013 and application thereof
  • Actinomycete strain SCAUT013 and application thereof
  • Actinomycete strain SCAUT013 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The separation and purification of embodiment 1 rhizosphere actinomycete SCAUT013

[0029] 1.1 Isolation of actinomycetes from the rhizosphere of Vitex glabrata in Liangshan

[0030] In 2018, the purple vetch was collected in Huidong County, Liangshan Yi Autonomous Prefecture, Sichuan Province. The excess soil on the root system was shaken off, and the root was placed in a 50mL sterilized centrifuge tube filled with 25mL phosphate buffer solution, and treated with ultrasonic waves (150W) After 10 minutes, the roots were taken out under sterile conditions, and the supernatant was removed by centrifugation at 3000g for 10 minutes. The sediment was rhizosphere soil, and made 10 -1 、10 -2 、10 -3 For gradient dilution, 100 ul of the suspension was uniformly spread on the modified Gowell's medium for separation, and each dilution concentration was repeated three times, and cultured in a constant temperature incubator at 28°C for 5-7 days. Pick the strains with typical acti...

Embodiment 2

[0047] The growth-promoting function screening of embodiment 2 rhizosphere actinomycetes SCAUT013

[0048] 2.1 Screening of growth-promoting function

[0049] 2.1.1 Determination of IAA Production Ability

[0050] Take 20 μL of the bacterial suspension and insert it into the ISP4 liquid medium containing 0.5mol / L tryptophan, repeat 3 times, set up a blank control, culture on a shaking table at 28°C for 3 days, centrifuge at 8000r / min for 24 hours, and take 1 mL of the bacterial solution Add 2mL of IAA chromogenic solution to the supernatant, react in the dark at 25°C for 30min, perform colorimetry at a wavelength of 530nm, and record the absorbance value. With the uninoculated liquid culture medium as the control zero adjustment, the indole acetic acid standard solution with the concentration of 0, 5, 20, 40, 60mg / l is the same as above to make the calibration, and calculate the concentration of IAA in the measurement solution.

[0051] 2.1.2 Determination of siderophore pro...

Embodiment 3

[0083] Embodiment 3 hydroponic experiment of purple sweet potato

[0084] 3.1 Hydroponics experiment

[0085] The actinomycete strains to be tested were first connected to the ISP 4 Slope activation, then transfer to ISP 4 The culture solution was cultivated overnight to the logarithmic phase, and the growth of the bacterial strain was detected by the plate method, and the bacterial content was calculated.

[0086] After soaking the seeds of Viola glabrata overnight, carry out surface disinfection, use sterile tweezers to grasp the seeds and plant them in a petri dish equipped with filter paper and sterile vermiculite, plant 10 seeds in each petri dish, and then add sterile water to keep moisture , and maintain air circulation. Sterilize the tweezers, filter paper, petri dishes, plastic cups, vermiculite, quartz sand, and low-nitrogen nutrient solution required for the experiment.

[0087] Low nitrogen nutrient solution formula: KNO 3 10.1g, KH 2 PO 4 2.2g, MnSO 4 ·H...

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Abstract

The invention discloses an actinomycete strain Micrococcus luteus SCAUT013 and application thereof, wherein the actinomycete strain is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO: 61507. According to the invention, the Micrococcus luteus SCAUT013 provided through separation and screening has growth promoting capabilities of generating IAA and siderophores, dissolving phosphorus, degrading cellulose and chitin and the like, also has stress resistance functions of salt resistance, acid and alkali resistance, drought resistance and the like, and can be applied to Vicia villosa Roth var .glabrescenscv .Liang shan to achieve the effect of promoting nodulation and growth of the Vicia villosa Roth var .glabrescenscv .Liang shan. According to the invention, the yield of forage grass is increased while the soil quality is improved, the tobacco planting soil is improved, and the actinomycete strain Micrococcus luteus SCAUT013 has good application potential in agricultural production.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to an actinomycetes strain SCAUT013 and its application. Background technique [0002] Liangshan Prefecture is one of the three major pastoral areas in Sichuan Province. Animal husbandry has a long history and is one of the main sources of cash income for farmers and herdsmen. Especially in high mountain areas, cash income from animal husbandry accounts for more than 70% of rural household income. Because Liangshan Prefecture has the climate characteristics of dry winter and spring, rainy summer, and distinct dryness and wetness, the problem of lack of green fodder for livestock during winter and spring is very prominent. However, in the mountainous areas around the basin of Liangshan Prefecture, especially in the Zhongshan and sub-alpine areas at an altitude of 1700-3200m, a large amount of land is left idle during winter and spring. Vicia villosa var.glabresens (Vicia vi...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P21/00C09K17/14C12R1/265C09K101/00
CPCC12N1/20A01N63/20C09K17/14C09K2101/00
Inventor 赵珂李静张紫薇陈强廖德聪辜运富邹立扣向泉桔余秀梅张凌子刘轶豪
Owner SICHUAN AGRI UNIV
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