Functional bone repair composite scaffold, preparation method and application

A composite scaffold and bone repair technology, applied in medical science, prosthesis, tissue regeneration, etc., can solve problems such as inflammatory response and tissue fibrosis, achieve the effects of promoting proliferation and differentiation, reducing tissue fibrosis, and ensuring uniformity

Pending Publication Date: 2022-02-11
WEIFANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the currently used exogenous composite scaffolds often cause inflammatory reactions after implantation in the body, leading to tissue fibrosis, the inventors have developed and prepared a "

Method used

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  • Functional bone repair composite scaffold, preparation method and application
  • Functional bone repair composite scaffold, preparation method and application
  • Functional bone repair composite scaffold, preparation method and application

Examples

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Embodiment 1

[0033] This embodiment provides a method of preparing a functional bone repair composite bracket, comprising the steps of:

[0034] S1: Remove the bone film of the distal surface of the pork bone femoral femoral, attached to the tissue and bone marrow, with a double steam rinsing femur 3-5 times with bone knife and chainsaw to divide the femur into 0.5cm 3 ~ 1cm 3 The bone block, then pulverized the bone block into 5mm with a pulverizer 3 ~ 8mm 3 Fine small bone particles;

[0035] S2: The bone particles in step S1 were cleaned 3 to 5 times in the step S1, respectively, and each cleaning was stirred at a magnetic stirrer, and each cleaning time was 0.5 to 1 h.

[0036] S3: Add a chloroform mixed solution to the bone particles after processing after step S2, stirred with a magnetic stirrer for 2 to 4 h to remove the surface of the particles and the internal fat component, wherein the volume ratio of chloroform and methanol in the mixed solution of chloroform mixed solution is 3: 1;...

Embodiment 2

[0046] This embodiment provides a method of preparing a functional bone repair composite bracket, and the specific steps are as follows:

[0047] S1: Remove the bone film of the distal surface of the pork bone femoral femur, attach the tissue and bone marrow, etc. 3 The bone block, then pulverized the bone block into 5mm with a pulverizer 3 Small particles;

[0048] S2: The bone particles in step S1 were cleaned 5 times in the step S1, each cleaning was stirred on the magnetic agitator, and each cleaning time was 0.5 h.

[0049] S3: Chloroform is added to the bone particles after processing after step S2: methanol (volume ratio 3: 1) solution, stirred with a magnetic stirrer for 4 h, removes the surface of the particles and more inner fat components;

[0050] S4: In the bone particles after treatment with step S3, the bone particles were added to the bone particles in step S3 were added to the NaCl solution having a mass fraction of 0.9% (w / v), and the mixer was stirred with a m...

Embodiment 3

[0060] This embodiment provides a method of preparing a functional bone repair composite bracket, and the specific steps are as follows:

[0061] S1: Eliminate the bone film of the distal surface of the pork bone femoral, attached to the tissue and bone marrow, etc. 3 The bone block, then pulverized the bone block into 5mm with a pulverizer 3 Fine small bone particles;

[0062] S2: The bone particles in step S1 were cleaned 5 times in the step S1, each cleaning was stirred on the magnetic agitator, and each cleaning time was 0.5 h.

[0063] S3: Chloroform is added to the bone particles after processing after step S2: methanol (volume ratio 3: 1) solution, stirred with a magnetic stirrer for 4 h, removes the surface of the particles and more inner fat components;

[0064] S4: In the bone particles after treatment with step S3, the bone particles were added to the bone particles in step S3 were added to the NaCl solution having a mass fraction of 0.9% (w / v), and the mixer was stir...

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Abstract

The invention relates to the technical field of bone tissue engineering, and discloses a preparation method of a functional bone repair composite scaffold. The method comprises the following steps: preparation of nano decellularized bone meal, preparation of a functional porous nano bone meal-gelatin-chitosan-dexamethasone composite scaffold, and crosslinking. The invention further discloses a nano bone meal-gelatin-chitosan-dexamethasone functional bone repair composite scaffold and application thereof. According to the invention, the functional bone repair composite scaffold has bone matrix components, cell adhesion sites, sufficient mechanical strength and a three-dimensional structure suitable for cell growth and proliferation and nutrient metabolism, the composite scaffold created by combining bone meal with gelatin and chitosan has good connectivity, the nano bone meal is uniformly dispersed in the pore wall, pores and skeleton of the scaffold, has a relatively high surface area, and facilitates the adhesion, growth and proliferation of osteoblasts, and due to introduction of the dexamethasone, the problems of tissue fibrosis and the like caused by inflammatory reaction caused by implantation of the allogenic scaffold can be effectively reduced, and the dexamethasone has an obvious promoting effect on proliferation and differentiation of the osteoblasts.

Description

Technical field [0001] The present invention relates to the field of bone tissue engineering, and in particular to a functional bone repair composite stent, preparation method and application. Background technique [0002] Because of trauma, tumor, infection, bone deficiency patients are very common, and about 3.5 million people in China have bone defects due to different reasons, and bone transplantation surgery is approximately 1.5 million cases. At present, there are three main methods for clinical repair bone defects: autologous bone transplantation, allogeneic bone transplantation and bone replacement. Autologous bones have been considered to be a gold standard for bone graft materials, but due to the source of donation, the donation site is not perfect and the complications are prone to secondary trauma of the bone bone, which limits its development. Although allogeneic bones do not exist in the supply zone and have sufficient bone source, it has similar biological activity...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/44A61L27/54A61L27/56
CPCA61L27/3608A61L27/3687A61L27/3691A61L27/54A61L27/56A61L27/446A61L2430/02A61L2300/216A61L2300/41A61L2300/412C08L89/00C08L5/08
Inventor 李文芳王乐王国娇王丹窦泽民高明铭
Owner WEIFANG MEDICAL UNIV
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