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Method for degrading carbon tetrachloride by two-step anaerobic biological enhancement method

A technology of carbon tetrachloride and anaerobic organisms, which is applied in the field of water pollution control, can solve the problem that the formation and accumulation heat of chlorinated intermediate products have not been fundamentally resolved, so as to avoid inhibition, ensure anaerobic environment, and simplify the operation process Effect

Pending Publication Date: 2022-03-11
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The anaerobic microbial degradation of dichloromethane is still lack of in-depth research, but several strains of anaerobic microorganisms that can ferment and degrade dichloromethane under anaerobic conditions have been found, such as Dhb sp.DCM 49A strain can ferment and degrade dichloromethane to Acetic acid and hydrogen, Dehalobacterium formicoaceticum DMC strain can fermentatively degrade dichloromethane into formic acid, acetic acid
However, the problem of formation and accumulation of toxic chlorinated intermediates has not been fundamentally resolved.

Method used

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  • Method for degrading carbon tetrachloride by two-step anaerobic biological enhancement method
  • Method for degrading carbon tetrachloride by two-step anaerobic biological enhancement method
  • Method for degrading carbon tetrachloride by two-step anaerobic biological enhancement method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Preparation of nano-sized zero-valent iron supported on activated carbon.

[0043] see figure 1 Preparation device, prepare 500mL mixed solution containing ferrous ion and activated carbon, which contains 20g / LFeSO 4 ·7H 2 O, 20g / L 200-mesh activated carbon and 10g / L polyvinylpyrrolidone, transfer the solution to a 1L solvent bottle, cover with an anaerobic rubber stopper, tighten the screw cap, vacuumize for 40min to remove oxygen, and place in a 200°C oven Leave it for 12 hours to load ferrous ions on activated carbon. After loading, the closed reaction vessel was placed on a magnetic stirrer and freshly prepared 100 mL of 1 M NaBH 4 The solution was slowly injected into the mixed solution containing ferrous ions with a syringe ( figure 1 ), magnetic stirring can make NaBH 4 Fully contact with ferrous ions and quickly discharge the generated hydrogen, and discharge a large amount of hydrogen generated by the reaction by vacuuming during the reaction process and m...

Embodiment 2

[0057] Chloroform anaerobic degradation enrichment culture and dichloromethane anaerobic degradation enrichment culture were obtained.

[0058]The river sediment collected from Xihe River in Shenyang City was used as the inoculation source. The content of organic matter in the above sediment is 8g / Kg, and the content of dissolved oxygen is 0.2mg / L. After collection, it is sealed and stored in a glass bottle.

[0059] Aliquot 100mL inorganic salt medium into 160mL glass serum bottles with N headspace 2 / CO 2 (80 / 20, v / v), add 5 mM sodium lactate as a carbon source, add 5 μL chloroform (liquid phase concentration 0.56 mM) or 5 μL dichloromethane (liquid phase concentration 0.73 mM) as an electron acceptor through a microsyringe, and ultrasonically dissolve. The culture medium and bottom mud were transferred into an anaerobic glove box, and 2 g of bottom mud was added to each bottle of culture medium as an inoculum source. The inoculated glass serum bottle was sealed with a ru...

Embodiment 3

[0065] Comparative experiments on the inhibitory effects of nano-zero-valent iron and activated carbon-loaded nano-zero-valent iron on the degradation ability of chloroform degradants in enrichment culture.

[0066] The specific experimental steps are as follows:

[0067] Aliquot 100mL inorganic salt medium into 160mL glass serum bottles with N headspace 2 / CO 2 (80 / 20, v / v), add 5mM sodium lactate as carbon source, 10mL hydrogen as electron donor, 5μL chloroform (liquid phase concentration 0.56mM) as electron acceptor, with 3% (v / v) transfer amount Inoculate with LSCF-1. Nano-sized zero-valent iron with an iron content of 12.5 mg and nano-sized zero-valent iron supported by activated carbon were added respectively, and cultured at 30° C. in the dark.

[0068] Chloroform and its degradation products in the culture were detected regularly by gas chromatography with flame ionization detector (GC-FID).

[0069] Depend on Figure 8 It can be seen that 2.7 μmol of dichlorometh...

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Abstract

The invention belongs to a water pollution treatment technology, aims at carbon tetrachloride polluted environment restoration under biological and non-biological process coupling, and particularly relates to a method for degrading carbon tetrachloride by a two-step anaerobic biological enhancement method. The method comprises the following steps: adding activated carbon loaded nano zero-valent iron into a to-be-treated pollutant sample containing carbon tetrachloride, when the carbon tetrachloride in the sample is degraded to 0.05-0.25 mM, adding chloroform to degrade the enrichment culture, treating under an anaerobic condition, and when dichloromethane is generated in the to-be-treated sample, adding the chloroform to degrade the enrichment culture, and adding dichloromethane into the system to degrade the enrichment culture, and carrying out enhanced treatment until carbon tetrachloride and other chloromethane in the system are removed. According to the implementation method for jointly degrading the carbon tetrachloride into the non-toxic product by using the nano zero-valent iron and the anaerobic enrichment culture, technical guidance and a new thought are provided for in-situ remediation of a carbon tetrachloride polluted site.

Description

technical field [0001] The invention belongs to water pollution treatment technology, and aims at the environmental restoration of carbon tetrachloride pollution under the coupling of biological and non-biological processes, specifically a method for degrading carbon tetrachloride by a two-step anaerobic bioaugmentation method. Background technique [0002] Carbon tetrachloride (Carbon tetrachloride, CT) is an excellent cleaning agent, coating and refrigerant widely used in the world. It is a persistent environmental pollutant with high degree of chlorination, high biological toxicity, stable chemical properties, and not easy to degrade. Once the human body is exposed to a small amount of carbon tetrachloride through the skin or mucous membranes, it will increase the burden on the liver, and exposure to a large amount of carbon tetrachloride will seriously damage the central nervous system. The dechlorinated degradation products of carbon tetrachloride, chloroform (Chlorofo...

Claims

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Application Information

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IPC IPC(8): C02F3/28C02F101/36
CPCC02F3/286C02F2101/36C02F2305/06C02F2003/003C02F2003/001Y02E50/30
Inventor 严俊王晶晶姜丽思李秀颖杨毅
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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