Tissue culture method of edible roses

A technology for tissue culture and edible roses, which is applied in the field of plant tissue culture, can solve the problems of industrialized seedling cultivation of edible roses, low seed germination rate, and easy breeding of diseases and insect pests, etc., and achieve the goal of improving the survival rate of transplanting, growing well, and improving growth Effect

Active Publication Date: 2022-03-22
明光现代农业科技合作推广服务中心 +1
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  • Application Information

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Problems solved by technology

[0003] However, the propagation of edible rose seedlings has low seed germination rate and low survival rate of seedlings, while cutting and grafting propagation can easily cause virus accumulation in the plant body, weaken the growth potential, and easily breed diseases and insect pests, and there are problems such as long reproductive cycle and small reproductive base. Make edible rose industrialized seedlings limited

Method used

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  • Tissue culture method of edible roses
  • Tissue culture method of edible roses
  • Tissue culture method of edible roses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Effects of Different Disinfection Treatments on the Contamination Rate and Start-up Rate of Explants

[0044] At noon in fine weather, select the branches that grow vigorously and without pests and diseases, remove the small thorns and petioles, rinse them under running water for 10 minutes, and clean the dust on the branches with a soft brush, taking care not to damage the axillary buds, and then put Add washing powder and shake it, then rinse it under running water for 30 minutes, and then do further disinfection on the ultra-clean workbench. First soak with 75% alcohol, rinse with sterile water 2 to 3 times, then sterilize with 4% sodium hypochlorite added with Tween 20, rinse with sterile water 3-4 times, dry the surface moisture of explants with sterilized filter paper , and finally cut the material into a single axillary bud stem section with a length of about 1 cm in a petri dish, pay attention to cutting the upper end flat, cutting the lower end obliquely, and i...

Embodiment 2

[0049] Effects of Different Hormone Combinations on Primary Culture

[0050] Using MS medium as the basic medium for primary culture, different concentrations of cytokinin 6-BA (1 mg / L, 2 mg / L, 3 mg / L) and different concentrations of auxin NAA (0.1 mg / L, 0.2 mg / L) were used. L, 0.3mg / L) to test, the test treatment method is shown in Table 2. 20 bottles were inoculated for each treatment, 3 explants were inoculated in each bottle, and repeated 3 times. After 30 days, the starting rate and bud height were counted.

[0051] Table 2 Hormone combinations of different concentrations in primary culture

[0052]

[0053] It can be seen from Table 3 (note: lowercase letters indicate a significant difference level of p=0.05, different letters indicate a significant difference (p<0.05)): with the increase of 6-BA concentration, the initiation rate of stem axillary buds also continues to increase ; With the increase of NAA concentration, the initiation rate of stem segment axillary ...

Embodiment 3

[0063] Effects of different anti-browning compounds on explant browning

[0064] Through experiments, it is found that the browning phenomenon is more serious in the tissue culture process of edible roses, which directly affects the normal growth of tissue culture seedlings. Therefore, this paper discusses the control effect of various anti-browning substances of different concentrations on explant browning, and the test treatments are shown in Table 6. 20 bottles were inoculated for each treatment, 3 explants were inoculated in each bottle, and repeated 3 times. After 30 days, the browning situation and growth status were counted.

[0065] Table 6 Anti-browning Treatment Test Design Scheme

[0066]

[0067] As can be seen from Table 7, the test results show that: adding vitamin CVC (0.5-1.5g / L), activated carbon AC (0.5-1.5g / L), and polyvinylpyrrolidone PVP (1-3g / L) in the medium can all be used in different To a certain extent, the occurrence of browning of stem segmen...

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Abstract

The invention provides a tissue culture method of edible roses, and relates to the technical field of plant tissue culture. The edible rose tissue culture method comprises the steps of explant disinfection, primary culture, subculture multiplication culture, rooting culture and acclimatization and transplantation. By adjusting the composition of the culture medium in each culture stage, the starting rate, the multiplication coefficient, the rooting rate and the transplanting survival rate of the edible rose tissue culture are improved, the pollution rate and the browning rate are reduced, and the rapid propagation efficiency of the edible rose is improved.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for tissue culture of edible roses. Background technique [0002] Edible roses are rich in nutrition, widely used, have high development value and broad application prospects, and are deeply loved by people. The demand is increasing year by year, and the market prospect is broad. Its planting and deep processing projects have been listed as key focus and support objects by Jiangsu, Yunnan and other provinces. [0003] However, the propagation of edible rose seedlings has low seed germination rate and low survival rate of seedlings, while cutting and grafting propagation can easily cause virus accumulation in the plant body, weaken the growth potential, and easily breed diseases and insect pests, and there are problems such as long reproductive cycle and small reproductive base. Make edible rose industrialized seedling cultivation be limited. The use of tis...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008
Inventor 王冬良陈友根吴宛滢苟云霞周琳
Owner 明光现代农业科技合作推广服务中心
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