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Application of horse bone marrow enzymolysis peptide in preparation of preparation for preventing osteoporosis

A technology for osteoporosis and osteoporosis, applied in the fields of peptide/protein composition, application, bone diseases, etc., can solve the problems of increased susceptibility to bone fragility fractures, unsatisfactory absorption effect, insufficient calcitonin secretion, etc. Broad application prospects and clinical value, prevention of osteoporosis, the effect of alleviating osteoporosis

Pending Publication Date: 2022-05-10
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the prior art, in the improvement of bone strength and bone structure-related indicators related to bone marrow protein, most of them use related proteins for direct treatment, and some technical solutions using abortion have a large molecular weight and the absorption effect is not ideal. In addition, postmenopausal women are due to ovarian Functional decline, decreased estrogen levels, secondary hyperparathyroidism, and insufficient calcitonin secretion, resulting in osteoclast bone resorption greater than osteoblast bone formation, resulting in low bone mass and bone microstructure It is characterized by degeneration, increased bone fragility and susceptibility to fracture, and most of the relevant treatment options in the prior art have adverse side effects

Method used

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  • Application of horse bone marrow enzymolysis peptide in preparation of preparation for preventing osteoporosis
  • Application of horse bone marrow enzymolysis peptide in preparation of preparation for preventing osteoporosis
  • Application of horse bone marrow enzymolysis peptide in preparation of preparation for preventing osteoporosis

Examples

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Effect test

Embodiment 1

[0067] Example 1: The use of horse bone marrow enzymolysis peptide in the preparation of preparations for preventing osteoporosis

[0068] This application provides the use of horse bone marrow enzymolysis peptide in the preparation of preparations for preventing osteoporosis.

[0069] Further, the present application provides the use of horse bone marrow enzymatic peptide in the preparation of preparations for preventing osteoporosis, for alleviating the occurrence of postmenopausal osteoporosis.

[0070] In the use of the horse bone marrow enzymatic peptide provided by the application in the preparation of preparations for preventing osteoporosis, the improvement is one or more selected from the following symptoms: preventing osteoporosis and promoting bone growth.

[0071] In the application of the equine bone marrow enzymatic hydrolyzed peptide provided by this application in the preparation of preparations for preventing osteoporosis, the improvement is one or more select...

Embodiment 2

[0080] Example 2: Preparation of Horse Bone Marrow Enzymatic Peptide

[0081] Alkaline protease, neutral protease, papain and pepsin were used to hydrolyze horse bone marrow protein under their respective optimal temperature and pH conditions, the uniform material-to-liquid ratio was 1:10, and the amount of enzyme added was 1.0%. The enzymatic hydrolysis time is 2h / 1 time, and 3 parallel experiments are done for each test result to measure the degree of hydrolysis and DPPH·scavenging ability, and determine the most suitable enzyme by comparison. The enzymatic hydrolysis conditions of different proteases are shown in Table 1.

[0082] Table 1: Enzymatic hydrolysis conditions for different proteases

[0083] Enzyme type / activity temperature / ℃ pH value Neutral protease / (100U / mg) 45 7.0 Alkaline protease / (400U / mg) 50 9.5 Papain / (1000U / mg) 55 6.5 Pepsin / (300U / mg) 37 2.8

[0084] 1. Single factor test

[0085] Get 5g horse bone marrow...

Embodiment 3

[0116]Example 3: SDS-PAGE analysis of horse bone marrow enzymatic peptides

[0117] SDS-PAGE of horse bone marrow protein hydrolyzate is attached Figure 8 As shown, the molecular weight of the standard protein is 4.1-66 kDa, and the concentrations of the separating gel and stacking gel are 15 and 5%, respectively. Prepare a certain concentration of horse bone marrow protein and its enzymatic hydrolyzate solution, accurately measure 5 μL of standard protein and protein samples into the spotting well, run at 75V for 30-60 min, and when the indicating front reaches the upper edge of the separation gel, adjust the voltage to 120V, stop electrophoresis when the indicated front reaches 3 / 4 of the separation gel. The results showed that horse bone marrow protein had various bands at 66, 45, 27, 20 and 9.5-14.4kDa. After enzymatic hydrolysis, the molecular weight of horse bone marrow protein changed greatly. The hydrolyzate products of four enzymes all showed bands at 9.5-14.4kDa, ...

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Abstract

The invention aims to provide application of horse bone marrow enzymolysis peptide in preparation of a preparation for preventing osteoporosis, horse bone marrow protein is adopted as a raw material of the horse bone marrow enzymolysis peptide, screening is carried out under the conditions of the optimum temperature and pH value of specific enzymolysis, and the polypeptide yield reaches up to 41.3%. Through electrophoretic analysis, the molecular weights of the original protein and the enzymatic hydrolysate thereof are mainly concentrated at 4.1-9.5 kDa. The prepared polypeptide is verified to have relatively strong free radical scavenging capability and an effect of preventing and treating osteoporosis of ovarian-removed female rats, and can effectively relieve occurrence of postmenopausal osteoporosis. The development of the horse bone marrow collagen peptide provides a raw material basis for research and development of health-care foods and natural medicines for preventing osteoporosis, and has wide application prospects and clinical values.

Description

technical field [0001] The invention belongs to the field of medicine development, relates to the preparation of medicines for preventing osteoporosis, and further relates to the technical field of enzymatic peptides of animal bone marrow and related uses thereof. Background technique [0002] Osteoporosis (osteoprorsis, OP) is one of the most common skeletal diseases, characterized by low bone mass and microstructural destruction of bone tissue, leading to increased bone fragility and susceptibility to fracture. After women's menopause, due to the gradual reduction of estrogen levels, bone breakdown will increase. The latest survey results show that the prevalence of osteoporosis in the population over 50 years old in my country is 19.2%, among which the prevalence rate of men is 6.0%, and the prevalence rate of women is as high as 32.1%. Obviously, osteoporosis has become another important health problem after diabetes and hypertension in the middle-aged and elderly peopl...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61P19/10A23L33/18A23J1/10A23J3/34
CPCA61K38/012A61P19/10A23L33/18A23J1/10A23J3/341A23V2002/00A23V2250/55A23V2200/306
Inventor 帕尔哈提·柔孜吾哈丽妮萨·麦麦提托合提古丽米热·阿巴拜克日则拉莱·司玛依马生军邓杰
Owner XINJIANG AGRI UNIV
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