35 results about "Post menopausal osteoporosis" patented technology

The invention belongs to the technical field of traditional Chinese medicines and relates to a traditional Chinese medicine composition for treating female postmenopausal osteoporosis and a preparation method thereof. In order to overcome the defects in the prior art, the invention provides the traditional Chinese medicine composition which can increase the bone mineral density of a patient suffering from postmenopausal osteoporosis and relieve secondary pain and inflammatory reaction, caused by the postmenopausal osteoporosis. The traditional Chinese medicine composition is mainly prepared from the following raw materials: palmleaf raspberry fruit, atractylodes macrocephala, radix paeoniae alba, tuber fleeceflower root, spina date seed, radix angelicae sinensis, radix codonopsis, radix morindae officinalis, radix acanthopanacis senticosi, rhizoma dioscoreae, poria cocos, radix rehmanniae preparata, radix puerariae, eucommia bark, herba menthae, rhizoma polygonati, fructus ligustri lucidi, epimedium herb, fructus lycii, fructus corni, calcium lactate, semen cuscutae, bovine bone powder, fortune's drynaria rhizome and radix glycyrrhizae. Pharmacological experiments and clinical trials prove that the traditional Chinese medicine composition has a significant effect for treating postmenopausal osteoporosis, so that the traditional Chinese medicine composition has a wide promotion value in the medical field.

The invention discloses an extraction method and the application of an urogenic stem cell exosome. The extraction method comprises the following specific steps: firstly, collecting urine, then carrying out first-time centrifugation, and adding PBS into a first residual liquid obtained after supernatant is removed; carrying out second-time centrifugation, resuspending the second-time residual liquid obtained after the supernatant is removed through a primary culture medium to obtain urogenic stem cell suspension solution; secondly, carrying out multiplication culture on the urogenic stem cellsand passing down to the second to the sixth generation for later use; thirdly, changing the culture medium obtained in the second step, then continuously culturing, collecting and culturing supernatant, centrifuging, filtering and carrying out centrifugal concentration; adding an exosome extraction reagent for extracting to obtain a resuspension solution of the urogenic stem cells. The urogenic stem cell exosome extracted by the invention is proved by animal experiments to have the advantages of good therapeutic effects on senile osteoporosis, postmenopausal osteoporosis and disuse osteoporosis.

The invention discloses a traditional Chinese medicine composition for treating postmenopausal osteoporosis, belonging to the field of traditional Chinese medicines. For overcoming the shortcomings of the prior art, the invention provides a traditional Chinese medicine that can increase the bone mineral density of a postmenopausal osteoporosis patient and also can relieve secondary pain and inflammatory response caused by postmenopausal osteoporosis. The traditional Chinese medicine composition is prepared from the following raw materials: black false hellebore, ligusticum wallichii, radix paeoniae alba, polygonum multiflorum, radix angelicae, angelica sinensis, codonopsis pilosula, gynostemma pentaphylla, acanthopanax root, the root of red-rooted salvia, poria cocos, prepared rhizome of rehmannia, the root of kudzu vine, asarum, mentha haplocalyx, xanthium sibiricum, glossy privet fruit, herba epimedii, the fruit of Chinese wolfberry, dogwood, radices sileris, the seed of Chinese dodder, bovine-derived power, astragalus membranaceus and liquorice. Pharmacology experiments and clinical experiments prove that the traditional Chinese medicine composition has a remarkable effect for treating postmenopausal osteoporosis, so the traditional Chinese medicine composition has a wide medical promotional value.

The invention belongs to the field of traditional Chinese medicine, and discloses a traditional Chinese medicine composition for treating female postmenopausal osteoporosis and a preparation method of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following traditional Chinese medicinal materials in parts by weight: 10 to 20 parts of eucommia ulmoides, 6 to 12 parts of herba epimedii, 5 to 10 parts of safflower carthamus, 5 to 10 parts of rhizoma corydalis, 5 to 10 parts of radix rehmanniae praeparata, 3 to 10 parts of pericarpium citri reticulatae viride, 3 to 10 parts of glossy privet fruit, 3 to 10 parts of rhizoma anemarrhenae, 3 to 6 parts of Chinese yam, 3 to 6 parts of adenophora stricta and 1 to 12 parts of liquorice. The traditional Chinese medicine composition provided by the invention is prepared from the natural traditional Chinese medicinal materials, is small in toxic and side effects, and simple in manufacturing process, has the efficacy of strengthening tendons and bones, activating blood to promote menstruation, nourishing yin and supplementing blood, invigorating spleen and replenishing Qi, and can be used for effectively treating female postmenopausal osteoporosis.

Compounds of the formulaR1 and R2 are independently alkyl of 1 to 6 carbon atoms, allyl, or substituted allyl of 3 to 6 carbon atoms; R3 is hydrogen, alkyl of 1 to 6 carbon atoms, or cycloalkyl of 3 to 10 carbon atoms; and R4 is phenyl or naphthyl substituted with alkyl of 1 to 6 carbon atoms, alkoxy of 1 to 6 carbon atoms or NR5R6; substituted or unsubstituted phenylalkyl wherein the alkyl group contains 1 to 6 carbon atoms; substituted or unsubstituted 5 to 10 membered heteroaryl having 1 to 3 heteroatoms selected from N, S and O; substituted or unsubstituted cycloalkyl of 3 to 10 carbon atoms; or substituted or unsubstituted cycloalkylalkyl of 4 to 10 carbon atoms; provided that phenyl or naphthyl is substituted with NR5R6 when R1 and R2 is alkyl, are useful in the treatment of various disorders associated bone loss by increased transcription and elevation of plasma calcitonin levels. Such disorders include, but are not limited to: Paget's Disease, post menopausal osteoporosis, senile osteoporosis, and glucocorticoid-induced osteoporosis.

The invention belongs to the field of traditional Chinese medicine, and discloses a traditional Chinese medicine composition for treating female postmenopausal osteoporosis and a preparing method thereof. The traditional Chinese medicine composition is prepared from, by weight, 10-25 parts of moringa oleifera leaves, 6-15 parts of radix dipsaci, 5-10 parts of codonopsis pilosula, 5-10 parts of rhizoma polygonati, 5-10 parts of radix rehmanniae praeparata, 3-9 parts of caulis spatholobi, 3-9 parts of radix clematidis, 3-9 parts of radix angelicae, 3-6 parts of Chinese yam, 3-6 parts of rhubarb and 1-10 parts of liquorice. The traditional Chinese medicine composition is prepared from natural traditional Chinese medicine, side effect is small, the preparing process is simple, the functions of enriching blood and stopping pain, strengthening tendons and bones, and promoting blood circulation to remove meridian obstruction are realized, and female postmenopausal osteoporosis can be effectively treated.

The invention relates to a preparation method and application of an animal marrow effective part. Raw materials in the method are fresh marrows contained in animal bones in slaughter houses of cattle,sheep, horses, camels or other animals, wherein a preparation method of a horse marrow effective component and application of the horse marrow effective component to osteoporosis are mainly used. According to the method, horse marrows are subjected to liquid nitrogen freezing, grinding, crushing, sieving, separation and petroleum ether degreasing treatment, water is used as a solvent, effective components in the horse marrows are extracted, and extracting solution is subjected to centrifugation, concentration, absolute ethyl alcohol precipitation, centrifugation and vacuum freezing drying toprepare crude extract; and the crude extract is respectively subjected to dialysis as well as separation and purification of ion exchange resin and the like to prepare the horse marrow effective component. The effective part is verified to have activities of changing hemorheology, improving microcirculation, reducing pain, having free radical scavenging capacity and the like. The research can provide reference for development of the animal marrow effective components as osteoporosis health-care products for treating or preventing various joint stiffness diseases and application of the animal marrow effective components to a medicine for treating oxidative stress mediated postmenopausal osteoporosis.

The invention provides a traditional Chinese medicine composition used for treating postmenopausal osteoporosis. The medicine composition comprises twotoothed achyranthes root, dipsacus asper, dried human placenta, common burreed tuber, colored mistlitoe herb, yerbadetajo herb, medlar, salvia miltiorrhiza, dragon's blood, licorice root, and ligusticum. When the traditional Chinese medicine composition is used for treating postmenopausal osteoporosis, advantages such as small side effect, good treatment effect, low cost, high curative rate, no medicine resistance, and convenient administration are provided.

The invention discloses a traditional Chinese medicine preparation for treating osteoporosis in postmenopausal women and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine preparation is prepared from ginkgo leaves, fortune's drynaria rhizome, pyrite, danshen root, prepared rehmannia root, hawthorn fruit, glossy privet fruit, common anemarrhena rhizome, belvedere fruit, adenophora stricta, and liquorice root. The traditional Chinese medicine preparation is prepared from purely natural traditional Chinese medicines, is low in side and toxic effects and simple in preparation process, has effects of strengthening tendons and bones, activating blood circulation to promote menstruation, nourishing yin, tonifying blood, and tonifying spleen and qi, and can effectively treat the osteoporosis in postmenopausal women.

The invention aims to provide application of horse bone marrow enzymolysis peptide in preparation of a preparation for preventing osteoporosis, horse bone marrow protein is adopted as a raw material of the horse bone marrow enzymolysis peptide, screening is carried out under the conditions of the optimum temperature and pH value of specific enzymolysis, and the polypeptide yield reaches up to 41.3%. Through electrophoretic analysis, the molecular weights of the original protein and the enzymatic hydrolysate thereof are mainly concentrated at 4.1-9.5 kDa. The prepared polypeptide is verified to have relatively strong free radical scavenging capability and an effect of preventing and treating osteoporosis of ovarian-removed female rats, and can effectively relieve occurrence of postmenopausal osteoporosis. The development of the horse bone marrow collagen peptide provides a raw material basis for research and development of health-care foods and natural medicines for preventing osteoporosis, and has wide application prospects and clinical values.

The edible ointment for preventing and treating postmenopausal osteoporosis is prepared from the following raw materials in parts by mass: 5 to 10 parts of fructus hippophae oil, 5 to 10 parts of cortex eucommiae extract and 25 to 50 parts of honeycomb. Compared with the prior art, due to the fact that postmenopausal osteoporosis is a chronic disease and often needs to be taken by patients for a long time, the scheme adopts a mode of using the ointment, and the ointment has the advantages of being ready to eat, good in taste, convenient to store, obvious in effect, free of obvious side effects and obvious in effect of improving symptoms of the postmenopausal osteoporosis patients and improving bone mineral density. Meanwhile, the dosage of western medicines can be reduced to a certain extent, the problem of side effects of the existing western medicines and the problem of mouth feel of taken traditional Chinese medicines are solved, and the treatment effect on postmenopausal osteoporosis and the medication compliance are improved to a great extent.

The invention discloses a postmenopausal osteoporosis related gene screening and function analysis method, which comprises the following steps: searching a gene expression chip related to postmenopausal osteoporosis from a GEO database, and obtaining a gene expression result; searching overlapped differential genes in the data sets of the two chips by utilizing a Venn graph; and carrying out gene enrichment function analysis by using a Metascap database and a DAVID database. According to the method, expression differential genes are downloaded by utilizing multiple online databases, the differential genes jointly expressed in two chip data sets are searched, bioinformatics analysis is carried out on the jointly expressed differential genes, and meaningful exploration and basis are provided for marker screening, molecular pathogenesis and the like related to the postmenopausal osteoporosis.

The invention provides a traditional Chinese medicine active ingredient formula for treating postmenopausal osteoporosis and application of the traditional Chinese medicine active ingredient formula, and belongs to the field of medicines. The traditional Chinese medicine active ingredient formula comprises traditional Chinese medicine active ingredients including lycium barbarum polysaccharide, icariin, polydatin, quercetin and salvianic acid A sodium which are calculated according to the weight (mg) ratio of (50-350): (6-100): (50-180): (1-10): (10-100). The traditional Chinese medicine active ingredient formula is reasonable in compatibility; the ingredients supplement each other; the traditional Chinese medicine active ingredient formula is definite in curative effect and high in innovativeness, and can be effectively applied to treatment of the postmenopausal osteoporosis; and repeated verification shows that the formula can remarkably increase the bone density of an animal with a postmenopausal osteoporosis model, improves the bone microstructure, is safe and effective, and can be used as a long-term medicine for the postmenopausal osteoporosis. The traditional Chinese medicine active ingredient formula can be further prepared into a medicinal preparation, is small in dosage form, is convenient to carry and take, has good economic and social benefits, and has a wide application prospect.