Preparation method of polymer/extracellular matrix micro-nano fiber composite material and product thereof
A technology of fiber composite materials and external matrix, applied in medical science, prostheses, absorbent pads, etc., can solve problems affecting tissue remodeling and repair, poor processability, insufficient mechanical strength, etc., to promote in situ tissue regeneration, improve Biological activity, effect of improving cell migration
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Embodiment 1
[0042] Example 1 Preparation of polycaprolactone (PCL)-placental extracellular matrix micro-nanofibrous tubular composite material
[0043] Obtain human placental extracellular matrix ( figure 1 ): Decellularization of human placental tissue by a combination of physical, chemical and biological methods. The specific method includes cutting the fresh (or frozen and thawed) human placental tissue into tissue blocks of 1×1 cm, and washing the residual blood in the tissue with normal saline. Afterwards, the tissue block was placed in a container containing 1% (m / v) sterile SDS (sodium dodecyl sulfonate) solution, treated at 4 °C with a shaker at 200 rpm for 24 h, the medium was changed every 6 h, and discarded. SDS solution, and use sterile normal saline to wash away the residual SDS. The tissue samples were treated with a mixture of sterile DNase (50 U / mL) and RNase enzyme (1 U / mL), shaken (100 rpm) for 12 h at room temperature, and washed with sterile saline until no enzyme so...
Embodiment 2
[0056] Example 2 Preparation of Polylactic Acid-Glycolic Acid Copolymer (PLGA)-Liver Extracellular Matrix Micro / Nano Fiber Membrane Composite
[0057] Obtaining porcine liver extracellular matrix: Decellularization of porcine liver tissue using a combination of physical, chemical and biological methods. The specific method includes cutting the purchased fresh porcine liver tissue into 1 mm thick slices, and washing the residual blood in the tissue with normal saline. Afterwards, the tissue was immersed in 0.1% peracetic acid solution, disinfected for 2 h, and then the residual peracetic acid solution was cleaned with sterile water. After that, put an appropriate amount of tissue pieces into a container containing 0.5% (m / v) sterile SDS solution, and treat at 4°C with a shaker at 200 rpm for 24 hours, change the medium every 6 hours, discard the SDS solution, and use sterile normal saline. Wash off the residual SDS. The tissue samples were treated with a mixture of sterile DN...
Embodiment 3
[0060] Example 3 Preparation of polyglycerol sebacate (PGS) / L-polylactic acid (PLLA)-small intestinal submucosa extracellular matrix micro-nanofiber membrane composite material
[0061] Obtaining extracellular matrix of porcine small intestinal submucosa: Decellularization of porcine small intestinal submucosa is carried out by combining physical, chemical and biological methods. The specific method includes: after rinsing the purchased small intestine of pigs, peeling and scraping the submucosa of the small intestine, cutting it into 10 cm long segments, and shaking and washing with sterile water on a shaker. Afterwards, an appropriate amount of tissue was soaked in a 0.1% peracetic acid solution, disinfected for 2 hours, and the residual peracetic acid solution was cleaned with sterile water. Then, sterile 0.25% (w / v) SDS was added, treated at 4°C with a shaker at 200 rpm for 24 hours, the medium was changed every 6 hours, the SDS solution was discarded, and the residual SDS...
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