Tripterygium wilfordii composition for inhibiting drug-resistant cancer as well as preparation method and application of tripterygium wilfordii composition
A composition, the technology of Tripterygium wilfordii, applied in the field of biomedicine, can solve the problems of liver damage, high pharmacological toxicity, skin pigmentation, etc., and achieve the effects of reduced toxicity, simple and easy operation of separation steps, and high repeatability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0036] The present invention provides a preparation method of the Tripterygium wilfordii composition. Compound combinations. .
[0037] In the present invention, the specific preparation method for the analysis sample of the Tripterygium wilfordii suspension cell extract is: after drying the Tripterygium wilfordii suspension cells, perform Soxhlet reflux extraction with 20 mL of ethyl acetate, extract 3 times, combine the extracts, Using a rotary evaporator, the solvent was recovered, the residue was evaporated to dryness and dissolved in 1 ml of methanol, 500 ul of methanol solution and 3 g of silica gel powder were mixed, and the sample was loaded. The amount of silica gel filler was 20 g. Rinse with 200mL petroleum ether eluent first, and discard the eluent. Then use 200mL of different ratios of petroleum ether:ethyl acetate eluent (9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8, 1:9, 0:10) for elution, and 10 fractions were collected. Use a rotary evaporator to recover the sol...
Embodiment 1
[0050] The combined inhibitory effect of the compound of Example 1 on cisplatin-resistant cells A549 / DDP
[0051] 1. Cell model establishment
[0052] Non-small cell lung cancer cells A549 and H1299, human large cell lung cancer cells H460 cisplatin-resistant cells, namely A549 / DDP and H1299 / DDP and H460 / DDP, and normal human lung bronchial epithelial cells 16HBE were used as cultured cells. Cultured in PRMI-1640 complete medium at 37°C, CO 2 The concentration of 5%, cultured to a certain number, can establish three cell models.
[0053] 2. Determination of the inhibition rate of the compound on cell proliferation
[0054] Take the three kinds of cells in the logarithmic growth phase obtained in step 1, respectively, to 5 × 10 4 The density of cells / mL was seeded in 96-well plates. 90 μL of cells were added to each well, and complete medium was set as blank control group, DMSO negative control, TP positive control and experimental groups added with different concentrations...
Embodiment 2
[0073] Example 2 Combined inhibitory effect of five compounds on cisplatin-resistant cells H1229 / DDP
[0074] The experimental design, five compounds, and the method for measuring the inhibitory effect on cisplatin-resistant cells H1229 / DDP are the same as those in Example 1.
[0075] from Figure 5 It can be seen that compared with the control group, the five compounds have significant inhibitory activity on H1299 / DDP cells, among which the inhibitory activity of compound P is stronger than that of TP, and its IC 50 It was found to be 12.80μg / ml. It was found that the proliferation inhibition effect of the two drugs was significantly improved. Compared with the single drug group, the cell inhibition rate was increased by 2.08, 3.24, 3.19, 2.36, and 2.06 times, showing a significant difference. The synergistic effect of TP combination therapy.
[0076] For H1299 / DDP cells, see Table 4 and Image 6 As shown, when the TP concentration dose is 2.5 μg / ml, the combined drug inde...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More