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Acinetobacter for denitrification and decomposing ammonia in wastewater

A waste water and ammonia nitrogen technology, applied in chemical instruments and methods, bacteria, biological water/sewage treatment, etc., can solve the problems of increasing water treatment costs, limiting popularization and application, and high requirements for cultivation technology, so as to achieve environmental protection and wide application Prospect, effect of high denitrification efficiency

Inactive Publication Date: 2005-02-23
INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(2) Free ammonia can affect the transfer of oxygen in the fish gills, causing a large number of fish deaths
[0007] At the same time, the traditional biological denitrification process also has many shortcomings that are difficult to solve by itself, which limits its large-scale popularization and application: (1) The nitrification process is completed in an aerobic environment, while denitrification is carried out in an anoxic process. Yes, this makes the whole denitrification process must go through two links of aerobic and anoxic
(2) The conversion process of ammonium salt to nitrite is completed by nitrous bacteria, and the growth rate of nitrous bacteria is chemoautotrophic bacteria, which leads to a long start-up time of the reactor and increases the cost of water treatment
For example: anammox bacteria are obligate anaerobic bacteria, which require high cultivation technology and cannot be applied to actual wastewater treatment projects. The disadvantage of being slow; heterotrophic nitrification and aerobic denitrification have high requirements for C / N, and greenhouse gas N will be generated during the treatment process 2 O, causing secondary pollution, etc.
Therefore, the research on these microorganisms is mostly limited to laboratory research and has not been applied to the actual water treatment process.

Method used

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  • Acinetobacter for denitrification and decomposing ammonia in wastewater
  • Acinetobacter for denitrification and decomposing ammonia in wastewater
  • Acinetobacter for denitrification and decomposing ammonia in wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Enrichment culture and isolation and purification of bacteria

[0033] 1. Enrichment culture of bacteria

[0034] Collect various environmental samples (activated sludge from various sewage treatment plants, ammonia-containing wastewater, domestic sewage, soil from chemical fertilizer plants, cultivated land, and various natural water bodies, etc.), use succinate-containing medium to aerate and enrich One to two weeks, during which time to supplement fresh medium.

[0035] Succinate medium formulation: (NH 4 ) 2 SO 4 0.5g, 2.17g sodium succinate, 50ml Vickers salt solution, add water to dissolve, and finally add distilled water to 1000ml (if it is a solid medium, add 2% agar).

[0036] Vickers salt solution composition: K 2 HPO 4 5.0g, MgSO 4 ·7H 2 O 2.5g, NaCl 2.5g,, FeSO 4 ·7H 2 O 0.05g, MnSO 4 4H 2 O 0.05g, add water to 1000ml.

[0037] 2. Primary screening and secondary screening of bacteria

[0038] Take 1ml of culture solution from the sample enric...

Embodiment 2

[0041] Denitrification bacteria obtained by isolation and purification

[0042] More than 10 strains of bacteria capable of aerobic ammonium oxidation were isolated by the method of limiting dilution and plate streak culture, and 6 strains with better effect were selected from them, which were numbered YY1-YY6 in sequence.

Embodiment 3

[0044] Comparison of denitrification effects of isolated bacteria

[0045] The isolated bacteria were inoculated in liquid medium for culture (in order to shorten the test time and the ammonia nitrogen concentration was halved) at 30°C and 120r / min shaker culture for 24 hours, and the changes in the ammonia nitrogen concentration were detected. The results are shown in Table 1.

[0046] Table 1. Comparison of ammonia nitrogen degradation effects of different strains screened

[0047] Strain number Ammonia nitrogen concentration (mg L -1 )

[0048] Blank control 58.49

[0049] YY-1 11.96

[0050] YY-2 3.58

[0051] YY-3 6.12

[0052] YY-4 13.11

[0053] YY-5 0.0

[0054] YY-6 14.17

[0055] It can be seen from Table 1 that among the isolated bacteria, YY-5 has a better denitrification effect, and was selected by us as the starting strain for the following research.

[0056] The strain was inoculated in liquid culture medium, cultured on a shaker at 30°C and 120r / min fo...

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Abstract

An acinetobactersp and ammonia nitrogen degrading method of waste water are disclosed. It includes: adding at least one of C1-C6 organic substances with 0.01-1wt%, adding Acinetobactersp and waste water with 10 to the power 5-10 to the power 12 bacterium / ml concentration, and aerating under gas-water ratio 10-15. It achieves environmental protection, simple process, high efficiency.

Description

technical field [0001] The invention relates to an Acinetobacter, in particular to an Acinetobacter capable of oxidizing ammonia nitrogen in waste water into nitrogen under aerobic conditions and a method for treating waste water with it. Background technique [0002] Nitrogen is an important pollutant in water pollution, and it mainly exists in two forms: organic nitrogen and inorganic nitrogen. The former includes proteins, polypeptides, amino acids, and urea, which are derived from domestic sewage and agricultural waste such as plant straw, livestock manure, etc., as well as certain industrial wastewater such as wool processing, tanning, printing and dyeing, and food processing. . The organic nitrogen is converted into inorganic nitrogen by microbial ammonification. Inorganic nitrogen in water refers to ammonia nitrogen, nitrite nitrogen and nitrate nitrogen, part of which is formed by the transformation of organic nitrogen through microbial decomposition, and part of i...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/20
Inventor 李君文孔庆鑫王新为金敏古长庆
Owner INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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