Method for separating lycorine from plant extract

Abstract

The invention relates to a kind of effective constituent separation method in vegetation extract, especially the method of separating lycorine from vegetation extract containing amaryllidaceae alkaloid. The industrial art is as follows: make vegetation extract containing amaryllidaceae alkaloid have pretreatment; adsorb and elute with cation exchange resin; track and detect the elution flowing composition; make the flowing composition in lycorine have concentration and enrichment; after get virgin product of lycorine free alkali, make it have recrystallization with organic solvent; finally, the lycorine free alkali crystallization with about 99% purity can be acquired.

Description

(1) Technical field

[0001] The invention relates to a method for extracting effective medicinal components of plants, in particular to a method for separating lycorine from other alkaloids from plant extracts containing amaryllidaceae alkaloids. (2) Background technology

[0002] Lycorine (lycorine) can inhibit the anaerobic glycolysis of mouse ascites cancer cells, and has a significant inhibitory effect on mouse lymphosarcoma, sarcoma 37 and rat lymphosarcoma, liver cancer cells; Hela cells, sarcoma 180, Ehrlich ascites Carcinoma, ascites liver cancer and Yoshida's sarcoma also have inhibitory effect. It is also the raw material for the preparation of anticancer drug lycorine oxide (AT-1840).

[0003] The structural formula of lycorine is shown in the figure below.

[0004]

[0005] Lycorine is extracted from natural plants. These plants are distributed in the genus Amaryllis, Manjusri, Narcissus, Tennis Flower and other fields. Among them, Lycoris is representative...

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