The invention relates to a regulating and control method based on a novel peristaltic pump. The peristaltic pump adopts pneumatic artificial muscles as an actuator and comprises a pump cover assembly,artificial muscle expansion components, flanges and air pipelines. A regulated and controlled control system comprises an air compressor, an air storage bottle, a motion control card, a relay module,a proportional pressure-regulating valve, two-position two-way reversing valves and a pressure sensor. Modules of the control system are connected from the air compressor, the air storage bottle, theproportional pressure-regulating valve, the two-position two-way reversing valves and the artificial muscle expansion components to a mechanical mechanism. During regulating and control, the output air pressure of the air compressor, the air pressure of the air storage bottle, the air pressure of a control loop, the working air pressure of the proportional pressure-regulating valve and the working air pressure of the pneumatic artificial muscles are verified sequentially, and the detection results are fed back. The peristaltic pump can transport a fluid medium and is provided with a self-insurance mechanism, and thus a pump body is not damaged during long-time working; the space occupied by the peristaltic pump is saved, and outdoor using is facilitated; the control system is stable and orderly; and the peristaltic pump works with the optimal efficiency, and the production efficiency is improved.