Method of producing fuel alcohol by kitchen garbage fermentation
A technology of food waste and fuel ethanol, applied in the direction of microorganism-based methods, biofuels, biochemical equipment and methods, etc., can solve the problems of long process routes and waste of energy, and achieve reduced investment costs, shortened production cycles, and production The effect of mild conditions
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Embodiment 1
[0011] Mix the crushed food waste with the distillation residue (or canteen waste water) at a ratio of 1:1, and use sterilized or non-sterilized methods, add 6kU of glucoamylase and 6% of yeast to 100g of waste, Maintain the fermentation temperature at 31°C, carry out ethanol fermentation in a closed fermentation shaker flask, and obtain a fermentation liquid containing 7% ethanol; ethanol purification: control the temperature of the fermentation liquid containing 7% ethanol at 80°C for distillation; after ethanol distillation The mash is centrifuged, and the residual liquid after centrifugation is reused in the food waste fermentation system to adjust the solid-liquid ratio for ethanol fermentation; the residue after centrifugation contains 85% water, which can be directly used as feed to feed livestock; or added to absorb water such as bran It is a non-toxic material, which can be dried and granulated to produce granular protein feed. The cost of raw materials is significant...
Embodiment 2
[0013] Mix the pulverized food waste and distillation residue (or canteen waste water) at a ratio of 1:0.5, after sterilization, add 10kU of glucoamylase and 10% yeast to 100g of waste, and ferment in a closed container at 35°C Fermented in shake flasks for 60 hours to prepare a fermented liquid containing 10.2% ethanol. Since food waste is rich in starches and other high-molecular carbohydrates that yeast cannot directly use, adding glucoamylase during fermentation can liquefy and saccharify the starch in the waste into polysaccharides or monosaccharides that yeast can use. Under the same process conditions as above, the experiment of fermentation without saccharification enzyme or fermentation after saccharification was carried out, and the results are shown in Table 1. The concentration of ethanol produced by the fermentation of kitchen waste without adding glucoamylase is less than 0.5%, which shows that glucoamylase effectively promotes the production of ethanol. The con...
Embodiment 3
[0017] According to Table 2, four fermentation methods are set up: A, B, C, and D. The fermentation substrate is prepared by mixing the crushed kitchen waste and distillation raffinate (or canteen waste water) at a ratio of 1:0.5, and 100g of waste is added with 10kU Glucoamylase and 10% saccharomyces were fermented in a closed fermentation shaker flask at 35°C for 60 hours. The results are shown in Table 2. Both B and D are fermented with non-sterilized ethanol, and no high-temperature sterilization of the fermentation substrate is required. Compared with the sterilized fermentation methods of A and C, the ethanol concentration in the fermentation liquid is almost the same, which shows that the non-sterilized fermentation is suitable for the process of producing fuel ethanol by fermentation of kitchen waste. The pH of the system was 4-5 at the beginning of the fermentation, and the lower pH also avoided bacterial contamination, and the pH dropped to about 4 after the ferment...
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