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Process of constructing nanometer biological device based on chemiluminescent resonant energy transfer principle

A technology of resonance energy transfer and biological nanotechnology, which is applied in the fields of chemiluminescence/bioluminescence, biological testing, and analysis through chemical reactions of materials, etc. In order to solve the problems of expensive equipment and other problems, it achieves the effects of low cost, easy operation, good water solubility and stability

Inactive Publication Date: 2007-10-03
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the complex structure of the fluorescence resonance energy transfer analysis system, the high price of the instrument, and the interference caused by the autofluorescence in the organism, its application is limited to a certain extent.
In addition, the current wavelengths of bioluminescence and chemiluminescence are in the visible spectrum region (generally 450-560 nanometers), and it is difficult to pass through animal tissues due to the absorption of animal tissues.

Method used

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  • Process of constructing nanometer biological device based on chemiluminescent resonant energy transfer principle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of sodium telluride hydride

[0024] Put 80 milligrams of tellurium powder, 80 milligrams of sodium borohydride and 2 milliliters of water in a 10 milliliter small flask, and immediately seal the mouth of the small reaction flask with a rubber stopper, and react at room temperature for 8 hours. Carefully transfer the supernatant in the flask to a 100 ml flask filled with degassed ultrapure water to prepare a 0.00625 mol / L sodium telluride hydride solution.

[0025] (2) Microwave Aqueous Mercaptopropionic Acid Assisted Synthesis of CdTe Quantum Dots

[0026] Mix 0.00125 mol / L cadmium chloride and 0.003 mol / L mercaptopropionic acid to form a solution, adjust the pH value to 8.0 with sodium hydroxide solution, pass nitrogen gas to remove oxygen for 30 minutes, stir vigorously and under the protection of nitrogen gas , inject sodium hydride telluride solution to form cadmium telluride monomer solution. The molar ratio of added divalent cadmium ions: sodium...

Embodiment 2

[0030] (1) The preparation of sodium telluride hydride is as described in Example 1.

[0031] (2) Aqueous phase synthesis of glutathione-modified CdTe quantum dots

[0032] Using water as a solvent, mix cadmium chloride with a concentration of 0.00125 mol / L and 0.0025 mmol / L glutathione, adjust the pH value of the solution to 9.0, and then inject 0.00625 mol / L sodium telluride hydride at a temperature of 25 degrees Celsius The mixture was stirred for 10 minutes to obtain a cadmium telluride precursor solution. The cadmium telluride precursor solution is heated at 80 degrees centigrade for 1-20 hours to obtain different quantum dots with a light emission range of 480-650 nanometers.

[0033] (3) Preparation of glutathione-modified cadmium telluride quantum dots-peroxidase bionanodevices

[0034] Will contain 2 x 10 -6 mol / L glutathione-modified CdTe quantum dots, 1×10 -3 Mole / L ethyl[3-(dimethylamino)propyl]carbodiimide hydrochloride and 1×10 -6 The mol / L peroxidase is add...

Embodiment 3

[0036] (1) The preparation of sodium telluride hydride is as described in Example 1.

[0037] (2) Aqueous Phase Synthesis of HgCdTe Quantum Dots

[0038] Dissolve 0.00125 mol / L cadmium chloride and 0.0025 mol / L mercuric chloride in 90 ml of ultrapure water, add 0.00625 mol / L mercaptopropionic acid and adjust the pH value to 10.0, inject 0.00625 mol / L tellurium 10 milliliters of sodium hydride solution, at this moment obtain mercury cadmium telluride precursor solution, and solution is orange-yellow. The solution was put into a microwave reactor for reaction, and heated at 130° C. for 30 minutes to obtain near-infrared mercury cadmium telluride quantum dots with a fluorescence emission peak at 800 nanometers and a quantum yield of 20%.

[0039] (3) Preparation of cadmium mercury telluride quantum dots-peroxidase bionanodevices

[0040] will contain 5 x 10 -5 mol / L mercaptopropionic acid modified cadmium mercury telluride quantum dots, 2.5×10 -3 Mole / liter of ethyl[3-(dimeth...

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Abstract

The present invention is process of constructing nanometer biological device based on chemiluminescent resonant energy transfer principle. The nanometer biological device consists of preoxidase and fluorescent quantum dot as energy receptor, the fluorescent quantum dot is cadmium telluride glowing based on chemiluminescent resonant energy transfer principle, and the preoxidase is connected with the fluorescent quantum dot by means of covalent bond. The nanometer biological device has high water solubility, high stability and adjustable emission wavelength, and generates strong light signal by means of chemiluminescent resonant energy transfer. The nanometer biological device has amino acids and carboxyl radical in the surface, is easy to covalent combine with various probe molecules, and may be used in detection and tracing of biological molecule and cell and tissue imaging mainly.

Description

technical field [0001] The invention relates to a method for constructing bionano devices based on the principle of chemiluminescence resonance energy transfer (CRET), which is mainly used for label detection and tracing of biomolecules, and imaging of cells, tissues and living bodies, and belongs to the field of biomolecular nanotechnology. The technical field of device fabrication. Background technique [0002] Fluorescent quantum dots (quantum dots, QDs, also known as nanocrystals) are a very important class of functional nanomaterials, which are inorganic nanoparticles composed of group II-VI or group III-V elements. When the diameter of these semiconductor nanocrystals is smaller than its Bohr radius, they exhibit special photoluminescent properties due to scale quantum effects and dielectric confinement effects. At present, fluorescent quantum dots have been successfully used in various fields of life sciences, mainly including immunoassay, DNA hybridization, live cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N33/48
Inventor 任吉存黄香宜
Owner SHANGHAI JIAO TONG UNIV
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