Preparation method of culture medium suitable for xylose-rich xylitol zymolysis production

A technology for culturing substrate and xylitol, applied in the field of microbial fermentation, can solve the problems of high steam consumption, increase production cost, destroy xylose, etc., and achieve the effects of overcoming steam and energy, large processing capacity and improving quality

Inactive Publication Date: 2008-03-12
NANJING YIBEIJIA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if the pH is adjusted to about 10, the hydrolyzed xylose will be destroyed and a lot of solid waste will be generated.
Moreover, due to the use of milk of lime to neutralize the inorganic acid in the hydrolyzate, it is easy to generate a large amount of gypsum precipitation, especially when it is concentrated for the first time, the scaling in the heater is serious, and this scaling can only be removed by mechanical means. After the equipment has been in operation for several days, it has to be stopped for descaling, which reduces the utilization rate and service life of the equipment, and the steam consumption is also high, which increases the production cost
[0004] Ion exchange resin can effectively remove harmful ions in the hydrolyzate, and is a very good post-treatment method for the hydrolyzate. However, due to its high cost, people have been trying to replace it with a combination of the aforementioned methods.
In addition, some people have tried the method of extracting with ether after neutralization of milk of lime. This method is relatively expensive, and no other reports have been seen.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1000 kg of air-dried corn cobs with less than 10% moisture. In the reaction kettle, add 10,000 kg of water and 5 kg of sulfuric acid, heat it to 100°C, keep it for 1 hour, and filter out the waste water. Add 6,000 kg of water and 60 kg of sulfuric acid, heat to 130°C, acidolyze for 2.5 hours, cool down, and filter. Add 30 kg of activated carbon to the acid solution for decolorization at 60°C for 1 hour, and filter. The filtrate passes through the electrodialysis tank to make the discharge conductivity less than 2000μS / cm. Then enter the reverse osmosis membrane (molecular weight 100MD) to concentrate, so that the output xylose is controlled at about 18%, and then enter the vacuum film to concentrate, the temperature is 75°C, so that the concentration of xylose in the concentrated solution reaches about 25%, and the concentration of furfural <400mg / L, The acid radical ion concentration is less than 800mg / L, and it enters the fermentation tank for batching. It is then ...

Embodiment 2

[0026] 1000 kg of air-dried straw with moisture less than 10%. In the reaction kettle, add 6000 kg of water and 1.2 kg of hydrochloric acid, heat it to 120°C, keep it for 2 hours, and filter out the waste water. Add 6,000 kg of water and 90 kg of hydrochloric acid, heat to 125°C, acidolyze for 2 hours, cool down, and filter. Add 30 kg of activated carbon to the acid solution for decolorization at 60°C for 1 hour, and filter. The filtrate passes through the electrodialysis tank to make the discharge conductivity less than 2000μS / cm. Then enter the reverse osmosis membrane (molecular weight 200MD) to concentrate, so that the output xylose is controlled at about 12%, and then enter the vacuum film to concentrate, the temperature is 75°C, so that the concentration of xylose in the concentrated solution reaches about 15%, and the concentration of furfural <400mg / L, The acid radical ion concentration is less than 800mg / L, and it enters the fermentation tank for batching. It is th...

Embodiment 3

[0028] 1000 kg of air-dried cottonseed hulls with less than 10% moisture. In the reaction kettle, add 15,000 kg of water and 75 kg of sulfuric acid, heat it to 60°C, keep it for 0.5 hours, and filter out the waste water. Add 10,000 kg of water and 180 kg of sulfuric acid, heat to 80°C, acidolyze for 3 hours, cool down, and filter. Add 50 kg of activated carbon to the acid solution for decolorization at 60°C for 1 hour, and filter. The filtrate passes through the electrodialysis tank to make the discharge conductivity less than 2000μS / cm. Then enter the reverse osmosis membrane (molecular weight 500MD) to concentrate, so that the output xylose is controlled at about 8%, and then enter the vacuum film to concentrate, the temperature is about 80°C, so that the concentration of xylose in the concentrated liquid reaches about 12%, and the concentration of furfural <400mg / L , acid radical ion concentration <800mg / L, enter the fermentation tank for batching. It is then fermented w...

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PUM

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Abstract

The present present inventiondiscloses a culture matrix preparation method, which contains xylitol and is applied to xylitol fermentation production. The culture matrix is obtained from organic matters, which are rich in celluloses and hemicelluloses and are hydrolytically detoxified after the steps of drying, washing by acid, acidolysis, decoloration, electrodialysis, concentration, etc. The present present invention is scrubbed with weak acid before acidolysis, which makes the content of impurities reduced and the quality of acidolysis solution improved. A plurality of acid radical ions and harmful ions in the acidolysis solution can be eliminated by a method of the electrodialysis, which can make the back reverse osmosis membrane concentration keep a high flux. With the back reverse osmosis membrane concentration, the cost can be effectively reduced, but the throughput is large. The defect that a plurality of steam and energies is consumed in the traditional steam heating concentration is overcomed. And the method is applied to industrial production. The vacuum thermal concentration can be further implemented after the reverse osmosis membrane concentration, which can eliminate volatile harmful materials and is beneficial for the biological fermentation and the xylitol production.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a preparation method of a culture substrate rich in xylose obtained from cheap agricultural by-products and suitable for fermenting and producing xylitol. Background technique [0002] Xylitol (molecular formula C 5 h 12 o 5 ) is a natural sweetener, the chemical name is "pentose", which is a five-carbon sugar and is a normal intermediate of carbohydrate metabolism in the human body. Xylitol integrates sweetener, nutrient, and therapeutic agent, and is widely used in industries such as national defense, medicine, chemical industry, daily chemical, paint, and food. At present, industrial scale production mainly adopts the chemical synthesis method of xylose hydrogenation, which requires high temperature, high pressure, flammable and explosive high-pressure hydrogen and a nickel catalyst that requires high purity of the solution. Capital construction investment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/236A23L27/30
Inventor 余永柱郑珩朱俊卿张彦方毕生毕贤
Owner NANJING YIBEIJIA TECH
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