Preparation method of culture medium suitable for xylose-rich xylitol zymolysis production
A technology for culturing substrate and xylitol, applied in the field of microbial fermentation, can solve the problems of high steam consumption, increase production cost, destroy xylose, etc., and achieve the effects of overcoming steam and energy, large processing capacity and improving quality
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Embodiment 1
[0024] 1000 kg of air-dried corn cobs with less than 10% moisture. In the reaction kettle, add 10,000 kg of water and 5 kg of sulfuric acid, heat it to 100°C, keep it for 1 hour, and filter out the waste water. Add 6,000 kg of water and 60 kg of sulfuric acid, heat to 130°C, acidolyze for 2.5 hours, cool down, and filter. Add 30 kg of activated carbon to the acid solution for decolorization at 60°C for 1 hour, and filter. The filtrate passes through the electrodialysis tank to make the discharge conductivity less than 2000μS / cm. Then enter the reverse osmosis membrane (molecular weight 100MD) to concentrate, so that the output xylose is controlled at about 18%, and then enter the vacuum film to concentrate, the temperature is 75°C, so that the concentration of xylose in the concentrated solution reaches about 25%, and the concentration of furfural <400mg / L, The acid radical ion concentration is less than 800mg / L, and it enters the fermentation tank for batching. It is then ...
Embodiment 2
[0026] 1000 kg of air-dried straw with moisture less than 10%. In the reaction kettle, add 6000 kg of water and 1.2 kg of hydrochloric acid, heat it to 120°C, keep it for 2 hours, and filter out the waste water. Add 6,000 kg of water and 90 kg of hydrochloric acid, heat to 125°C, acidolyze for 2 hours, cool down, and filter. Add 30 kg of activated carbon to the acid solution for decolorization at 60°C for 1 hour, and filter. The filtrate passes through the electrodialysis tank to make the discharge conductivity less than 2000μS / cm. Then enter the reverse osmosis membrane (molecular weight 200MD) to concentrate, so that the output xylose is controlled at about 12%, and then enter the vacuum film to concentrate, the temperature is 75°C, so that the concentration of xylose in the concentrated solution reaches about 15%, and the concentration of furfural <400mg / L, The acid radical ion concentration is less than 800mg / L, and it enters the fermentation tank for batching. It is th...
Embodiment 3
[0028] 1000 kg of air-dried cottonseed hulls with less than 10% moisture. In the reaction kettle, add 15,000 kg of water and 75 kg of sulfuric acid, heat it to 60°C, keep it for 0.5 hours, and filter out the waste water. Add 10,000 kg of water and 180 kg of sulfuric acid, heat to 80°C, acidolyze for 3 hours, cool down, and filter. Add 50 kg of activated carbon to the acid solution for decolorization at 60°C for 1 hour, and filter. The filtrate passes through the electrodialysis tank to make the discharge conductivity less than 2000μS / cm. Then enter the reverse osmosis membrane (molecular weight 500MD) to concentrate, so that the output xylose is controlled at about 8%, and then enter the vacuum film to concentrate, the temperature is about 80°C, so that the concentration of xylose in the concentrated liquid reaches about 12%, and the concentration of furfural <400mg / L , acid radical ion concentration <800mg / L, enter the fermentation tank for batching. It is then fermented w...
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