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Preparation method of biosensor based on composite nanometer interface

A biosensor and interface technology, applied in the field of biochemical medicine, can solve the problems of insufficient resolution and high development cost, and achieve the effects of high sensitivity, excellent properties and easy operation.

Inactive Publication Date: 2008-09-10
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the disadvantages of insufficient resolution and high development costs in the prior art, the present invention proposes a composite nanoparticle that integrates the advantages of different nanomaterials, and at the same time combines it with carbon fiber paper to form a biosensor based on a composite nanointerface. This biosensor has high sensitivity and can be widely used in biomedical fields such as biomolecular recognition and cancer cell detection

Method used

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  • Preparation method of biosensor based on composite nanometer interface
  • Preparation method of biosensor based on composite nanometer interface
  • Preparation method of biosensor based on composite nanometer interface

Examples

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Embodiment 1

[0017] Example 1 Preparation of a biosensor based on composite nano-interface

[0018] Titanium isopropoxide and carbon nanotubes were respectively dissolved in 2-propanol solution at a mass ratio of 30:70, and the titanium isopropoxide solution was mixed with about 1 Add dropwise to the carbon nanotube solution at a rate of ~2 drops / second, adjust the pH of the solution to 3.0 with dilute sulfuric acid, react at room temperature for 2 to 3 hours, then centrifuge the reaction product, wash it repeatedly with distilled water, and finally remove the solvent by evaporation TiO2-carbon nanotube composite particles were obtained.

[0019] Dissolve titanium dioxide-carbon nanotube composite particles, conductive carbon black, and polyvinylidene fluoride in the solvent 1-methyl-2-pyrrolidone at a mass ratio of 85:10:5, spread the mixture evenly on carbon fiber paper, and put After drying in a vacuum drying oven at 120°C for 24 hours, the carbon fiber paper was taken out, and cut acc...

Embodiment 2

[0021] Example 2 Preparation of a biosensor based on composite nano-interface

[0022] Dissolve titanium isopropoxide and carbon nanotubes in ethanol solution at a mass ratio of 10 to 40:60 to 90, and stir the titanium isopropoxide solution at a rate of about 1 to 2 drops / second under vigorous stirring by a magnetic stirrer. Slowly add it dropwise to the carbon nanotube solution, adjust the pH value of the solution to 3 with dilute sulfuric acid, centrifuge the reaction product after reacting at room temperature for 2 to 3 hours, wash it repeatedly with distilled water, and finally remove the solvent by evaporation to obtain titanium dioxide-carbon nanotubes Composite particles. The mass ratio of titanium isopropoxide to carbon nanotubes can be any value in the range of 10:90 to 40:60, such as 10:90, 13:87, 60:40 and so on. Titanium isopropoxide and carbon nanotubes can also be dissolved in propanol or butanol.

[0023] Dissolve titanium dioxide-carbon nanotube composite par...

Embodiment 3

[0024] Example 3 Preparation of a biosensor based on composite nano-interface

[0025] Dissolve 0.5-4 mg of carbon nanotubes in 0.1-3.0 wt % of chloroauric acid (HAuCl 4 ) aqueous solution, sonicate for 5 minutes to disperse evenly, dilute to 100 mL with twice distilled water, and heat to boiling while stirring with a magnetic stirrer. Then add 4 mL of sodium citrate solution and continue heating for 5-10 minutes, the color of the solution changes from light yellow to reddish brown, and when the color of the solution no longer changes, the solvent is evaporated to obtain gold-carbon nanotube composite particles. Wherein the carbon nanotube can be any value in 0.5-4 mg, such as 0.5 mg, 1.3 mg or 4 mg. Chlorauric acid is any value from 0.1 to 3.0 wt%, such as 0.1%, 2.3% or 3.0%. Carbon nanotubes and chloroauric acid solution can be mixed in any proportion.

[0026]Dissolve gold-carbon nanotube composite particles, conductive carbon black, and polyvinylidene fluoride in the so...

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Abstract

The invention belongs to the field of biosensors and provides a preparation method for a biosensor based on a composite nanometer interface. The first step follows that a carbon nano-tube composite particle is prepared on a carbon nano-tube by a sol-gel method; the second step follows that the carbon nano-tube composite particle prepared in the first step, electric carbon black and polyvinylidene fluoride are dissolved in the solvent of 1-methyl-2-pyrrolidone, wherein, the weight of the carbon nano-tube composite particle accounts for 80 percent of the total weight of the carbon nano-tube composite particle, the electric carbon black and the polyvinylidene fluoride, the mixture is uniformly smeared on a carbon fiber paper, taken out after being dried and cut according to requirement so as to be convenient for experimental use. In the preparation method of a biosensor based on a composite nanometer interface, the carbon nano-tube composite particle can either be a titanium dioxide-carbon nano-tube composite particle or a gold-carbon nano-tube composite particle, wherein, the carbon nano-tube is a carbon nano-tube that has a single tube wall or a carbon nano-tube that has a plurality of tube walls. The biosensor has high sensitivity.

Description

technical field [0001] The invention relates to the technical field of biochemical medicine, in particular to a preparation method of a biosensor. Background technique [0002] Nanobiotechnology combines nanomaterials science, physics and biotechnology, and plays an important role in gene therapy, biomedicine, the construction of biological nanostructures and the study of the relationship between the structure and function of biological macromolecules. Carbon nanotube (CNT), as a new type of nano-carbon material that has emerged in the past ten years, due to its unique physical and chemical properties, such as low density, high specific modulus, high strength, good electrical conductivity and temperature conductivity, etc. , has become one of the hottest research objects in nanotechnology. In recent years, the biological effects of carbon nanotubes have aroused great interest, and the exploration and research for the purpose of biological applications are increasing rapidly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48C12Q1/00
Inventor 王雪梅沈琴朴秀吉姜晖刘善京郭大东陈宝安
Owner SOUTHEAST UNIV