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Method for testing effective ingredients of Gegen Qinlian medicine

A detection method and technology of Gegen Qinlian, applied in the field of quality control of traditional Chinese medicines, can solve the problem that there is no simultaneous detection of Gegen Qinlian drugs, etc., and achieve the effects of easy control, good reproducibility, and reduction of composition changes and measurement errors.

Inactive Publication Date: 2008-10-08
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no detection method that can simultaneously detect the contents of the six components in Pueraria chinensis

Method used

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  • Method for testing effective ingredients of Gegen Qinlian medicine
  • Method for testing effective ingredients of Gegen Qinlian medicine
  • Method for testing effective ingredients of Gegen Qinlian medicine

Examples

Experimental program
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Effect test

example 1

[0036] 1. Instruments and reagents

[0037] Instrument: Agilent1100 high performance liquid chromatography (online degasser, quaternary pump, autosampler, column thermostat, diode array detector); AgilentChemStation chromatography data workstation; AB250D one hundred thousandth balance (USA DENVER company) , Milli-pore-Q ultrapure water preparation instrument.

[0038] Reference substances: puerarin, daidzin, baicalin, wogonoside, palmatine and berberine.

[0039] Sample to be inspected: the sample to be inspected is Gegenqinlian tablet, which is self-made according to the item of Gegenqinlian tablet in the 2005 edition of "Chinese Pharmacopoeia".

[0040] Other reagents: acetonitrile (chromatographically pure), ammonium acetate, glacial acetic acid (analytical pure).

[0041] 2. Method

[0042] (1) Preparation of mixed reference substance solution: accurately weigh appropriate amount of puerarin, daidzin, baicalin, wogonoside, palmatine, and berberine reference substance, di...

example 2

[0053] 1. The instrument and reagents used for detection are the same as in Example 1; the sample to be tested is Gegen Qinlian pellets, which are self-made according to the item of Gegen Qinlian pellets in the 2005 edition of "Chinese Pharmacopoeia".

[0054] 2. Method

[0055] (1) Preparation of reference substance solution: Accurately weigh an appropriate amount of puerarin, daidzin, baicalin, wogonoside, palmatine, and berberine reference substance, dissolve with methanol, constant volume, shake well, filter membrane, A reference substance solution with a content of 1 μg / ml was prepared.

[0056] (2) Preparation of sample solution: Accurately weigh an appropriate amount of Gegen Qinlian pellets, heat and ultrasonically dissolve them completely with 100% methanol at 100°C, and prepare a sample solution with a concentration of 0.2mg / ml, shake well, and filter through a microporous membrane .

[0057] (3) The chromatographic column is filled with octadecylsilane bonded sili...

example 3

[0065] 1. The apparatus and reagents used for detection are the same as in Example 1; the extract of Pueraria chinensis of the sample to be tested is prepared under the item of Pueraria chinensis micropills in the 2005 edition of "Chinese Pharmacopoeia": take Scutellaria baicalensis and Coptis chinensis in the prescription of Pueraria qinlian. The percolation method (appendix 10) under extractum and extractant item, use 50% ethanol as solvent respectively, carry out percolation after soaking for 24 hours, collect percolation liquid, reclaim ethanol, suitably concentrate; Scutellaria baicalensis, coptis dregs and roasted licorice, continue to decoct twice, 1.5 hours each time, combine the decoction, filter, concentrate the filtrate to an appropriate amount, add the above concentrate, continue to concentrate to a thick paste, dry under low temperature and reduced pressure, and grind into Finest powder.

[0066] 2. Method

[0067] (1) Preparation of reference substance solution:...

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Abstract

The invention provides a method to detect effective ingredients of radices puerarire, radices scutellariae and coptis chinensis medicine (Gegen Qin Lian Medicine) by high efficiency liquid chromatogrophy. The method consists of the following steps: firstly, a sampling liquid to be detected and a mixing control solution are prepared, which are then respectively detected by the following methods: a chromatographic column stuffed by octadecyl silane bonded silica gel is injected by the sample size of 1 to 20ul, the column temperature is controlled between 10 to 40 DEG C, then gradient elution is carried out by a mobile phase which is composed of phase A and phase B at a flow velocity of 0.3 to 1.5ml / min according to the stipulated procedure; moreover, multi-wavelength ultraviolet is employed to detect the eluent so as to obtain the high efficiency liquid chromatograms of the detected medicines and the control solution; finally, the high efficiency liquid chromatograms of the detected medicines and the control solution are compared so as to determine the effective components of puerarin, daidzin, wogonoside, baicalin, palmatine and berberine and the contents thereof. The phase A in the method of the invention is ammonium acetate solution with the concentration of 0.01 to 0.1mol / l, and the phase B is acetonitrile.

Description

technical field [0001] The invention relates to a quality control method of traditional Chinese medicine, in particular to a quality control method of Pueraria chinensis medicine. Background technique [0002] Gegen Qinlian Decoction was originally recorded in Zhang Zhongjing's "Treatise on Febrile Diseases" in the Eastern Han Dynasty. It is made by decocting four herbs of Pueraria root, scutellaria, coptis, and licorice. Syndrome of body heat and lower benefit, irritability in the chest and abdominal cavity, thirst in the mouth, panting and sweating. In recent years, medical scientists at home and abroad have proved through experimental research that Gegen Qinlian Decoction has pharmacological effects such as antipyretic, antibacterial, antiviral, antispasmodic, inhibiting gastrointestinal motility, anti-hypoxia, anti-arrhythmia, and enhancing the immune function of the body. Commonly used prescriptions for treating bacillary dysentery, typhoid fever, acute and chronic gas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/718G01N30/02A61P31/04A61P31/12A61P29/00A61P9/06A61P37/04A61P1/12A61P1/00
Inventor 罗佳波谭晓梅宋丽军
Owner SOUTHERN MEDICAL UNIVERSITY
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