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CD34 antibody or CD133 antibody surface orientation fixing method of titanium and titanium alloy cardiovascular implantation device

An implanted device and directional fixation technology, applied in prosthesis, medical science, etc., can solve problems such as thrombus formation and social loss, and achieve the effect of improving efficiency and beneficial anticoagulant function

Inactive Publication Date: 2012-06-13
深圳市金瑞凯利生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although drug-coated stents can reduce the incidence of restenosis, the drug in the coating inhibits the proliferation of endothelial cells while inhibiting the proliferation of smooth muscle cells, leaving the stent exposed, leading to inflammation, coagulation and even thrombosis
[0004] At present, there are about 200,000 patients in our country who need artificial heart valve replacement surgery every year due to serious valvular disease. Most of these patients are young and middle-aged. If the valve cannot be replaced in time, it will cause immeasurable losses to the society.

Method used

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  • CD34 antibody or CD133 antibody surface orientation fixing method of titanium and titanium alloy cardiovascular implantation device
  • CD34 antibody or CD133 antibody surface orientation fixing method of titanium and titanium alloy cardiovascular implantation device
  • CD34 antibody or CD133 antibody surface orientation fixing method of titanium and titanium alloy cardiovascular implantation device

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. A method for directional immobilizing CD34 antibody on the surface of a titanium alloy cardiovascular implant device, the steps of which are as follows:

[0036] A. Activation treatment

[0037] Soak the titanium alloy cardiovascular implant device in 0.5mol / L NaOH solution at 40°C for 1 hour, take it out and place it in deionized water at 60°C for 1 hour, then dry it in air at 30°C; irradiate with ultraviolet light for 1 hour minute.

[0038] B. Fixation of avidin:

[0039] Immerse the implanted device after the activation treatment in step A in physiological saline with avidin concentration of 0.1 mg / ml for 1 hour, rinse it with physiological saline after taking it out, and dry it with nitrogen gas;

[0040] C. Immobilization of biotinylated protein A:

[0041] Immerse the implanted device treated in step B in 0.1% albumin solution, incubate at room temperature for 5 minutes, then immerse it in physiological saline containing 0.01 mg / ml biotinylated protein A fo...

Embodiment 2

[0045] 1. A method for directional immobilizing CD34 antibody on the surface of a titanium cardiovascular implant device, the steps of which are as follows:

[0046] A. Activation treatment

[0047] Soak the titanium cardiovascular implant device in 5mol / L NaOH solution at 80°C for 24 hours, take it out and place it in deionized water at 90°C for 24 hours, dry it in air at 90°C, and irradiate it with ultraviolet light for 30 minutes.

[0048] B. Fixation of avidin:

[0049] Immerse the implanted device after the activation treatment in step A in phosphate buffer solution with avidin concentration of 5.0 mg / ml for 12 hours, rinse it with phosphate buffer solution after taking it out, and dry it with nitrogen gas;

[0050] C. Immobilization of biotinylated protein A:

[0051] Immerse the implanted device treated in step B in 5% albumin solution, incubate at room temperature for 40 minutes, then immerse it in phosphate buffer solution containing 5 mg / ml biotinylated protein A f...

Embodiment 3

[0055] 1. A method for directional immobilizing CD133 antibody on the surface of a titanium alloy cardiovascular implant device, the steps of which are as follows:

[0056] A. Activation treatment

[0057] Soak the titanium alloy cardiovascular implant device in 3mol / L NaOH solution at 70°C for 12 hours, take it out and place it in deionized water at 70°C for 20 hours, then dry it in the air at 80°C, and irradiate it with ultraviolet light for 20 hours. minute.

[0058] B. Fixation of avidin:

[0059] Immerse the implanted device after the activation treatment in step A in phosphate buffer solution with avidin concentration of 1.0 mg / ml for 6 hours, rinse it with phosphate buffer solution after taking it out, and dry it with nitrogen gas;

[0060] C. Immobilization of biotinylated protein A:

[0061] Immerse the implanted device treated in step B in 1% albumin solution, incubate at room temperature for 40 minutes, and then immerse it in phosphate buffer containing 0.1 mg / ml...

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Abstract

The invention discloses a method which adopts the surface of a titanium and titanium alloy cardiovascular implantation device to fix CD34 antibody or CD133 antibody directionally, and comprises the following main steps: A. activation treatment is carried out, so as to form hydroxyl on the surface; B. a layer of avidin is adsorbed on the activation surface of the step A; C. a layer of biotinylatedprotein A is adsorbed by the avidin layer; D. on the surface of the protein A layer obtained, a layer of specific antibodies of the membrane protein of endothelial progenitor cells- CD34 antibodies or CD133 antibodies are fixed directionally, so the titanium and titanium alloy cardiovascular implantation device can capture the endothelial progenitor cells in blood after being implanted into the human body, thereby leading to rapid endothelialization of the implantation device surface and leading the implantation device to have excellent anticoagulant function and restenosis occurring resistance function.

Description

Technical field [0001] The invention relates to a biochemical modification method of titanium and titanium alloy cardiovascular implantation devices. Background technique [0002] Cardiovascular system diseases are one of the important diseases that endanger human health. Artificial implantation of titanium and titanium alloy cardiovascular implants (vascular stents or heart valves) is an effective method for the treatment of cardiovascular diseases. At present, more than 2 million patients with coronary heart disease need percutaneous puncture coronary angioplasty every year in the world, and 70% of them need to implant vascular stents. In 2006, more than 100,000 patients received vascular stent implantation in my country. Heart valve disease in my country accounts for about 30% of heart disease patients, and more than 100,000 critically ill patients must undergo artificial heart valve replacement every year. [0003] In 1987, Sigwart used titanium and titanium alloy intr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/54A61L27/06
Inventor 黄楠李全利陈佳龙陈诚陈俊英王进杨苹孙鸿冷永祥万国江赵安莎游天雪吴熹
Owner 深圳市金瑞凯利生物科技有限公司