Arachidonic acid content detecting method in milk and milk products

A technology for the detection of arachidonic acid and its detection method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems affecting the accuracy of detection results, etc., and achieve the effects of improved recovery rate, simple and convenient operation, and short extraction time

Inactive Publication Date: 2009-05-06
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Claims
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AI Technical Summary

Problems solved by technology

[0004] However, in the sample processing step of this method, the test is carried out directly after methyl esterification and standing still, and further experiments have proved that AA in n-hexane is not completely extracted after the methyl esterification reaction, which will affect the detection results the accuracy of

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  • Arachidonic acid content detecting method in milk and milk products
  • Arachidonic acid content detecting method in milk and milk products
  • Arachidonic acid content detecting method in milk and milk products

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1 The making of arachidonic acid (AA) standard curve

[0030] Chromatographic conditions: flame ionization detector; sample injection temperature: 260°C; detector temperature: 300°C; °C / min to 215°C and hold for 30 minutes. Capillary column: CP-WAX58 or a column with equivalent performance. Carrier gas: high-purity nitrogen, air and hydrogen.

[0031] The n-hexane used in this example is a chromatographically pure reagent. AA standard solution was purchased from sigma with a purity of ≥99.99%. After dissolving the AA methyl ester standard with n-hexane, prepare samples with concentrations of 10ppm, 50ppm, 100ppm, 200ppm, 400ppm, and 800ppm and inject them separately. According to the measured data, draw the standard between the AA content and the detection result curve, the equation is A std = 263.76C, R = 0.9999. Among them, A std Indicates the chromatographic peak area of ​​the standard solution, and C indicates the concentration of the standard solu...

Embodiment 2

[0032] Example 2 Detection of AA content in fatty acid samples

[0033] 1) Accurately weigh 0.5g fatty acid sample, add n-hexane, dissolve the sample with n-hexane, set the volume to 10ml scale, accurately measure 2ml of the solution after constant volume, add 0.5ml anhydrous methanol-potassium hydroxide (4mol / l ) was fully shaken, and left to stand for 20 minutes for esterification reaction, and water was added to the n-hexane solution to 25ml.

[0034] 2) Stand still, and after the organic phase is clarified, take the supernatant, filter, and inject a sample into a gas chromatograph for detection.

[0035] Chromatographic conditions: flame ionization detector; sample injection temperature: 260°C; detector temperature: 300°C; °C / min to 215°C and hold for 30 minutes. Capillary column: CP-WAX58 or a column with equivalent performance. Carrier gas: high-purity nitrogen, air and hydrogen. The result obtained by detection is compared with the standard curve obtained in Example...

Embodiment 3

[0044] Example 3 Detection of AA content in milk powder

[0045]A) Accurately weigh 1.0000g of milk powder sample into a flat-bottomed flask, shake and dissolve with 10ml of 65°C water, add 2ml of 25% ammonia water, shake gently, return to distillation in a water bath at 65°C for 15 minutes, rinse the return distillation tube with 10ml of ethanol, Cool to room temperature. Add it to Mao's ester extraction bottle, and add 2 drops of Congo red indicator. B) After rinsing the flask with 25ml of ether, add it to an ester extraction bottle and shake it 100 times. Then wash the flask twice with 25ml of petroleum ether, and put the washing liquid in an ester extraction bottle, and shake it 50 times. Wash the bottle cap with a mixture of diethyl ether and petroleum ether. After standing for 30 minutes to separate the layers, the organic phase was separated. Repeat the extraction once with 5ml of absolute ethanol, 15ml of ether, and 15ml of petroleum ether, and combine the organic ...

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Abstract

The invention relates to a method for checking the arachidonic acid content in milk and milk products. After a sample is processed, a gas-phase spectrum meter is used for check. The sample processing method comprises the following steps: step 1, normal hexane and absolute-methanol-potassium-hydroxide are added into the sample for carrying out etherification reaction; step 2, the solution obtained in step 1 is centrifuged or stands until the organic phase is clarified to extract the supernatant fluid to obtain a solution to be checked. Water is added into the solution after the etherification after step 1 or before step 2. For a solid or liquid sample, the sample is firstly processed as following: step A, ammonia water is added into the sample, and ethanol is added after reaction; step B, ether and sherwood oil are added for layer separation in a standing mode, the organic phase is taken and concentrated until a dried state, and the sample concentrated liquid is obtained. The method of the invention has the advantages of short extracting time, complete extraction, simple and convenient operation and high accurate rate, and is suitable for checking a great number of samples.

Description

technical field [0001] The invention relates to a method for detecting the content of arachidonic acid in milk and dairy products, especially a method for detecting the content of arachidonic acid in milk and dairy products by using gas chromatography, especially about the detection of milk and dairy products by gas chromatography Improvements in sample handling steps in the process of arachidonic acid content. Background technique [0002] Arachidonic acid (AA) is an essential higher fatty acid for the human body. It is essential for maintaining the functions of various tissues in the human body. When it is deficient, it can cause a series of symptoms, including growth retardation, infertility, and mental retardation. In order to meet the growth needs of the human body, it has become a consensus to obtain an appropriate amount of fatty acids from the diet, and various supplementary foods such as milk powder and beverages have emerged as the times require. Therefore, it is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/02
Inventor 赵英
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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