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Chitosan nano-microspheres product and preparation method thereof

A chitosan and nano-micro technology, applied in the direction of making medicines into special physical or ingestible devices, pharmaceutical formulas, medical preparations of non-active ingredients, etc., can solve the problem of uneven and uncontrollable nutrient and cell density , high emulsification pressure, etc., to achieve good drug activity retention, high drug embedding rate, and good dispersion

Active Publication Date: 2010-03-24
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The heterogeneity of microspheres and microcapsules will also bring many difficulties to the practical application of chitosan and its derivative microspheres or microcapsules.
For example, as an enzyme immobilization and protein separation medium, due to the non-uniform particle size, small microspheres will gather in the gap between large microspheres in the column, which will increase the separation pressure, and in severe cases, the separation will not be possible. ; As a cell culture carrier, uneven particle size will lead to uneven nutrient and cell density, resulting in uneven cell growth; as a drug sustained release carrier, the embedding rate of the drug will be greatly reduced due to the instability of the emulsion, and because The particle size of microspheres or microcapsules is not uniform, which makes the targeting of drugs poor in systemic administration, resulting in a greatly reduced bioavailability of drugs in the body
[0007] In order to overcome the non-uniform and uncontrollable particle size of chitosan microspheres prepared by traditional preparation methods, our laboratory developed a direct membrane emulsification method
Because the basic condition for the microporous membrane emulsification method to obtain uniform emulsion droplets is that the interfacial tension between the dispersed phase and the membrane surface is large enough, therefore, although the membrane with a small pore size can be used to prepare uniform and controllable chitosan and Its derivative nano-microspheres, but adopting this method to prepare smaller nano-microspheres (such as below 5 microns) emulsification speed is very slow, and the required emulsification pressure is large, so it is not suitable for large-scale preparation of chitosan with small particle size Nano-microspheres, and it is difficult to prepare chitosan nanospheres below 400 nanometers by this method

Method used

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  • Chitosan nano-microspheres product and preparation method thereof
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  • Chitosan nano-microspheres product and preparation method thereof

Examples

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preparation example Construction

[0070] In some embodiments, the preparation method of the present invention includes the following steps: dissolving a certain amount of chitosan in 1% aqueous acetic acid solution, and the resulting solution is used as the water phase; taking a certain amount of oil-soluble emulsifier and dissolving The compatible organic phase is used as the oil phase; after the water phase and the oil phase are mixed rapidly, the W / O type pre-emulsion is prepared by homogeneous emulsification or mechanical stirring; the pre-emulsion is quickly pressed through the passed or untreated The W / O emulsion with uniform particle size can be obtained from the hydrophobically treated glass microporous membrane; in order to make the particle size more uniform, the emulsion obtained each time can be used as a pre-emulsion, which is repeatedly pressed out through the membrane pores under higher pressure; A certain amount of cross-linking agent or precipitating agent is added to the emulsion, or the emuls...

Embodiment 1

[0093] Soak the hydrophilic SPG glass membrane with a pore size of 1.4 microns in a mixed oil phase of liquid paraffin and petroleum ether at a volume ratio of 5:7 overnight or ultrasonically for half an hour to fully wet the membrane pores. Accurately take a certain amount of chitosan and dissolve it in 1% acetic acid aqueous solution, make it fully dissolve under magnetic stirring to obtain chitosan acetic acid aqueous solution, its concentration is 0.5wt%, this solution is centrifugally removed insoluble impurity under 2000rpm, retains The supernatant was used as the aqueous phase for later use. Add the oil-soluble emulsifier PO-500 into 60 ml of a mixture of liquid paraffin and petroleum ether (5:7 by volume) at a concentration of 4 wt%, and stir until completely dissolved as an oil phase. The above-mentioned chitosan acetic acid aqueous solution of 2ml is mixed with oil phase and is emulsified for 1 minute under third gear (6000rpm) with homogeneous emulsifier, forms pre-...

Embodiment 2

[0102] Chitosan nano-microspheres are prepared using the same device and method as in Example 1, wherein the difference is that the SPG membrane with a pore size of 1.4 microns is treated through hydrophobic treatment, and the cross-linking and curing reaction of emulsion droplets is carried out under heating conditions. Be 45 ℃, the average particle diameter of gained chitosan nano-microsphere is 350.0 nanometers, and C.V. is 14.18%, and the scanning electron micrograph of gained microsphere is as follows Figure 8 As shown, the results showed that the prepared chitosan microspheres were relatively uniform in particle size.

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Abstract

The invention provides a chitosan nano-microspheres product in which the average grain size of the chitosan nano microspheres is from tens of nanometers to ten micrometers and the grain size distribution coefficient C.V. is less than 20%. The invention also provides a preparation method and an application of the chitosan nano microspheres.

Description

technical field [0001] The invention relates to a chitosan nano-microsphere product and a preparation method thereof, in particular to a chitosan nano-microsphere product with substantially uniform particle diameters below 10 microns and a preparation method thereof. Background technique [0002] Chitosan is a polysaccharide composed of glucosamine and N-acetylglucosamine copolymer, which is the product of deacetylation of chitin. Chitosan can be dissolved in dilute acid solution due to the free amino group on the molecular chain, so It has a wider range of uses than chitin. As a cationic biopolymer, chitosan has bioadhesion, biocompatibility and biodegradability. The uniqueness of chitosan makes it widely studied and applied in the field of biomedicine, such as drug carrier , cell culture carrier, immobilized enzyme carrier, protein separation medium, etc. [0003] Many studies have found that chitosan is an ideal material for the preparation of oral nano-microspheres and...

Claims

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Application Information

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IPC IPC(8): A61K47/36C08B37/08A61J3/00
Inventor 马光辉王连艳苏志国吕丕平魏炜卫强张岳玲
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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