Method for preparing matrix microcapsule loaded with water-soluble substance
A technology of water-soluble substances and microcapsules, which is used in drug delivery, tissue engineering, and biomedical materials. It can solve the problem of biocompatibility and degradability of organic macromolecules without special consideration, and the macromolecular components of microcapsule walls are not easy to control. , poor controllability of colloidal particles, etc., to achieve the effect of easy control of shape and particle size distribution, good slow-release effect, and easy complete removal
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Embodiment 1
[0042] Dissolve sodium hyaluronate (HA) in 500 mL of ammonium bicarbonate solution with a concentration of 0.01 mol / L, and make the final concentration of HA 0.25 mg / mL or 0.5 mg / mL or 3.0 mg / mL. After the dissolution is complete, Mix quickly with 500mL manganese sulfate solution with a concentration of 0.001mol / L. After 30 minutes, the reaction system was centrifuged to collect the precipitate to obtain manganese carbonate particles (expressed as HA / MnCO3) combined with sodium hyaluronate macromolecules. 3 ), washed three times with deionized water by centrifugation (5000rpm, 10min), and stored in a 1.5mL centrifuge tube for later use. HA / MnCO 3 The scanning electron microscope photo of the hybrid particle template is detailed in figure 1 a. The sodium hyaluronate macromolecule was labeled with the fluorescent dye DTAF, and DTAF-HA / MnCO was prepared as described above. 3 Hybrid colloidal particle template, observed under confocal laser microscope, see figure 2 a.
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Embodiment 2
[0047] Dissolve sodium hyaluronate (HA) in 500mL of sodium bicarbonate solution with a concentration of 0.05mol / L to make a final concentration of HA of 5mg / mL. Mix quickly. After 30 minutes, the reaction system was centrifuged to collect the precipitate, and after washing, the manganese carbonate particles (expressed as HA / MnCO) combined with sodium hyaluronate macromolecules were obtained. 3 ). HA / MnCO 3 The hybrid particles were used as templates, and HA matrix microcapsules were prepared according to Example 1. Mix 1mL matrix microcapsule suspension with 1mL insulin solution with a concentration of 3mg / mL, adjust the pH to 1.5, collect the microcapsules by centrifugation after standing at room temperature for 8 hours, wash with buffer three times, and redisperse in buffer. Using the matrix microcapsule suspension not loaded with insulin as the blank control, it was proved by ultraviolet spectrophotometry that there was a high concentration of protein accumulated in the ...
Embodiment 3
[0049] Dissolve sodium alginate (ALG) in 500 mL of ammonium bicarbonate solution with a concentration of 0.01 mol / L, and make the final concentration of ALG 0.25 mg / mL or 0.5 mg / mL or 4.0 mg / mL. After the dissolution is complete, mix with 500mL manganese sulfate solution with a concentration of 0.001mol / L was mixed rapidly. After 30 minutes, the reaction system was centrifuged to collect the precipitate, and the manganese carbonate particle template (expressed as ALG / MnCO 3 ), washed three times with deionized water by centrifugation (5000rpm, 10min), and stored in a 1.5mL centrifuge tube for later use. ALG / MnCO 3 The scanning electron microscope photo of the hybrid particle template is detailed in figure 1 b. The sodium alginate macromolecule was labeled with the fluorescent dye DTAF, and DTAF-ALG / MnCO was prepared as described above. 3 Hybrid colloidal particle template, observed under confocal laser microscope, see figure 2 b.
[0050] 15-30 mg of the above-mentioned...
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