Method for separating and purifying gamma-aminobutyric acid (GABA) from glutamine decarboxylase enzymolysis liquid

A glutamylamine decarboxylase enzymatic hydrolyzate, glutamylamine decarboxylase technology, applied in the field of bioengineering, can solve the problems of long γ-aminobutyric acid process steps, incomplete separation and purification, limited separation ability, etc. Conducive to the utilization of solidified concentrates, avoiding secondary pollution and high concentration multiples

Active Publication Date: 2010-10-20
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above process, high-speed centrifugation not only consumes a lot of energy, but also has limited separation capacity.
For the production of bulk raw materials, plate and frame filtration needs to add a large amount of filter aids, and the components in the filtrate are complex, the separation accuracy is low, the filtrate will cause a large burden or pressure on the subsequent purification, serious leakage of electrodialysis materials, h

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example 1

[0020] The glutamylamine decarboxylase solution prepared by microbial fermentation, using sodium glutamate as the substrate, after enzymatic hydrolysis to obtain an enzymatic hydrolysis solution with a GABA content of 2-9g / L. The enzymatic hydrolysis solution is subjected to organic ultrafiltration with a cut-off molecular weight of 1000Da The membrane is filtered and clarified, the temperature of the material liquid is controlled at 20~60℃, the operating pressure is 0.1~0.3MPa, the flow rate of the membrane surface is 2~5.5m / s, when the concentration is 1 time, add dialysis water, the amount of dialysis water is 2 times enzymatic hydrolysis The liquid volume is concentrated after 5 times. The total filtration yield is 95-99%, the concentration factor is 5 times, the amount of filtrate is 2.5 times of enzymolysis solution, and the amount of concentrated solution is 0.5 times of enzymolysis solution. The filtrate is pumped into the cation and anion exchange res...

Example Embodiment

[0021] Example 2

[0022] Glutamine decarboxylase solution prepared by microbial fermentation, using sodium glutamate as a substrate, and enzymatic hydrolysis solution with a GABA content of 2-9g / L is obtained. The enzymatic hydrolysis solution uses a stainless steel ultrafiltration membrane with a membrane pore size of 20nm Filter and clarify, the temperature of the feed solution is controlled at 20~60℃, the operating pressure is 0.4~0.8MPa, the flow rate of the membrane surface is 2~5.5m / s, when the concentration is 5 times, add dialysis water, and the amount of dialysis water is 0.5 times the enzymatic hydrolysis solution The amount is concentrated after 10 times. The total filtration yield is 95-99%, the concentration factor is 10 times, the filtrate volume is 1.4 times the enzymolysis solution, and the concentrated solution volume is 0.1 times the enzymolysis solution. The filtrate is pumped into the cation and anion exchange resin columns in sequence, the column temperatur...

Example Embodiment

[0023] Example 3

[0024] The glutamylamine decarboxylase solution prepared by the extraction process uses glutamic acid as the substrate. After enzymolysis, the enzyme hydrolysis solution with the GABA content of 2-9g / L is obtained. The enzyme hydrolysis solution is carried out with a microfiltration membrane with a molecular weight of 100,000 Da. Filter and clarify, control the material liquid temperature at 20~60℃, the operating pressure is 0.4~0.8MPa, the membrane surface flow rate is 2~5.5m / s, when the concentration is 5 times, add dialysis water, the dialysis water volume is 0.4 times the enzymatic hydrolysis volume , End after concentration 10 times. The total filtration yield is 95-99%, the concentration factor is 10 times, the filtrate volume is 1.3 times the enzymolysis solution, and the concentrated solution volume is 0.1 times the enzymolysis solution. The filtrate is pumped into the cation-anion mixed bed ion exchange system, the column temperature is less than 40°C...

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Abstract

The invention relates to a method for separating and purifying gamma-aminobutyric acid (GABA) from glutamine decarboxylase enzymolysis liquid. The method comprises the following steps of: delivering glutamine decarboxylase enzymolysis liquid which is abundant in GABA into a film separating system for filtering and clearing; when concentrating to 2-10 times, adding dialyzing water for dialyzing, wherein the final filtering liquid amount is 1 to 3 times of enzymolysis liquid amount; pumping filtering liquid into an ion exchange system for desalinating; delivering desalinated liquid into decolorizing resin or an active carbon column for decolorizing; concentrating decolorized clear liquid by an evaporator; and drying or crystallizing and recrystallizing to obtain a GABA product. Compared with the prior art, the separating and purifying process provided by the invention is simple and reasonable and has short procedure, and the GABA obtained by separation has high purity, shallow color, good dispersibility and high total yield; and the invention has the advantages of low running cost, high filtering precision, high concentration times, high concentration of enzyme concentrates, and thelike, is beneficial to the recycling or solidified resource utilization of the enzyme concentrates, and prevents secondary pollution.

Description

technical field [0001] The invention relates to separation and extraction technology in the field of bioengineering, in particular to a method for separating and purifying gamma-aminobutyric acid (GABA) from glutamine decarboxylase hydrolyzate. Background technique [0002] γ-aminobutyric acid (γ-amino butyric acid, GABA), also known as aminobutyric acid, is a naturally occurring non-protein amino acid, and its molecular formula is NH 2 CH 2 CH 2 CH 2 COOH, molecular weight 103.1, melting point 202°C (decomposes under rapid heating), easily soluble in water, slightly soluble in hot ethanol, insoluble in other organic solvents. γ-aminobutyric acid exists in animal brains and plant germs in small amounts in nature, such as about 0.1-0.3mg / g in brain tissue, 35mg / kg in brown rice, and 40mg / kg in bean leaves. [0003] γ-aminobutyric acid can pass through the central nervous system to promote vasodilation, calm the nerves, lower blood pressure, inhibit atherosclerosis, smooth...

Claims

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Application Information

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IPC IPC(8): C07C229/08C07C227/40
Inventor 赵黎明
Owner EAST CHINA UNIV OF SCI & TECH
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