Method for separating and purifying gamma-aminobutyric acid (GABA) from glutamine decarboxylase enzymolysis liquid

Abstract

The invention relates to a method for separating and purifying gamma-aminobutyric acid (GABA) from glutamine decarboxylase enzymolysis liquid. The method comprises the following steps of: delivering glutamine decarboxylase enzymolysis liquid which is abundant in GABA into a film separating system for filtering and clearing; when concentrating to 2-10 times, adding dialyzing water for dialyzing, wherein the final filtering liquid amount is 1 to 3 times of enzymolysis liquid amount; pumping filtering liquid into an ion exchange system for desalinating; delivering desalinated liquid into decolorizing resin or an active carbon column for decolorizing; concentrating decolorized clear liquid by an evaporator; and drying or crystallizing and recrystallizing to obtain a GABA product. Compared with the prior art, the separating and purifying process provided by the invention is simple and reasonable and has short procedure, and the GABA obtained by separation has high purity, shallow color, good dispersibility and high total yield; and the invention has the advantages of low running cost, high filtering precision, high concentration times, high concentration of enzyme concentrates, and thelike, is beneficial to the recycling or solidified resource utilization of the enzyme concentrates, and prevents secondary pollution.

Description

technical field [0001] The invention relates to separation and extraction technology in the field of bioengineering, in particular to a method for separating and purifying gamma-aminobutyric acid (GABA) from glutamine decarboxylase hydrolyzate. Background technique [0002] γ-aminobutyric acid (γ-amino butyric acid, GABA), also known as aminobutyric acid, is a naturally occurring non-protein amino acid, and its molecular formula is NH 2 CH 2 CH 2 CH 2 COOH, molecular weight 103.1, melting point 202°C (decomposes under rapid heating), easily soluble in water, slightly soluble in hot ethanol, insoluble in other organic solvents. γ-aminobutyric acid exists in animal brains and plant germs in small amounts in nature, such as about 0.1-0.3mg / g in brain tissue, 35mg / kg in brown rice, and 40mg / kg in bean leaves. [0003] γ-aminobutyric acid can pass through the central nervous system to promote vasodilation, calm the nerves, lower blood pressure, inhibit atherosclerosis, smooth...

AI Technical Summary

Problems solved by technology

In the above process, high-speed centrifugation not only consumes a lot of energy, but also has limited separation capacity.

For the production of bulk raw materials, plate and frame filtration needs to add a large amount of filter aids, and the components in the filtrate are complex, the separation accuracy is low, the filtrate will cause a large burden or pressure on the subsequent purification, serious leakage of electrodialysis materials, heavy maintenance and difficult collection The efficiency is too low, the separation and purification are not complete, the production cost is high, and the product quality is poor

[0006] In summary, the existing separation and purification methods for the production of γ-aminobutyric acid by fermentation have the disadvantages of long process steps, serious material run-off, low production yield, poor product quality, and high operating costs.