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Bio-diatomite composite flocculant

A technology of composite flocculants and biological flocculants, applied in the field of water treatment, can solve the problems of low treatment efficiency and secondary pollution of flocculants, and achieve the effects of high treatment efficiency, reduced dosage, and reduced sludge production

Inactive Publication Date: 2010-12-01
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a high-efficiency and environment-friendly bio-diatomite compound for the low treatment efficiency of the existing sewage treatment process, the effluent water quality needs to be improved after advanced treatment, and the addition of flocculant is easy to cause secondary pollution. flocculant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Screening of flocculating microorganism strains

[0039] Take 10g sample of activated sludge from the aerobic pond of Suojin Village Sewage Treatment Plant in Nanjing, and put it in a basic salt medium (1.5g K 2 HPO 4 , 0.5g KH 2 PO 4 , 1.0g NH 4 NO 3 , 0.2g MgSO 4 , 1.0g NaCl / L water, pH7.0) culture at 30°C for 24h, filter the culture solution with sterile filter paper, rinse the filter paper with sterile water, wash the flocs on the filter paper, and add it to the sterile basal salt medium In, the sample is enriched and cultured for 4 times and then pure bacteria are separated. The enrichment solution was coated on Luria-Bertani (LB) nutrient medium and cultured at 30°C for 48 hours. A single colony with a smooth and sticky surface was selected and its flocculation activity was determined. The strain with higher flocculation activity was selected as the re-screening strain. Through repeated screening and purification, three strains of the best flocculant-produc...

Embodiment 2

[0041] Fermentation culture of flocculent microorganisms: Under aseptic conditions, 3 strains (CGMCC No. 3515, CGMCC No. 3514 and CGMCC No. 3516) were activated on the inclined plane, respectively, inoculated with LB medium shake flasks, and the shaker speed 200r / min, culture time 50h to mid logarithmic growth period, inoculate the first-level seed tank at 5% of the inoculum, and the second-level seed tank will be scaled up and cultivated step by step, and the bacterial count can reach 1.0-1.2×10 9 / ml of fermentation liquid. The fermentation medium is glucose 1%, ammonium sulfate 0.15%, dipotassium hydrogen phosphate 0.05%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.002%, NaCl 0.02%, calcium chloride 0.01%, and the rest is tap water, pH 7.0, Fermentation at 28~30℃, tank pressure 0.5kg / cm 2 , The ventilation volume is the volume of the fermentation broth m 3 / Air volume m 3 ·Min 1:0.8, stirring speed of 150r / min, pH value of about 6.5, aerobic fermentation for 65h...

Embodiment 3

[0043] Centrifuge the fermentation broth of Example 2 at 5000 rpm for 30 min after culturing for 2 days to the stable growth stage to remove the bacteria (precipitation), concentrate (conventional ultrafiltration, reduced pressure) the supernatant, and add the equivalent supernatant to the supernatant 0.1% by weight CaCl 2 And 2.5 times the volume of the supernatant of absolute ethanol, let stand at room temperature for 40 minutes, then centrifuge at 12000g for 15 minutes, discard the supernatant, get precipitate 1 for use; dissolve precipitate 1 with 3.0%, pH5 sodium chloride solution (Sodium chloride solution can just dissolve the precipitate 1), adsorb with a 12% cetyltrimethylammonium bromide solution that is 1 times the volume of the sodium chloride solution, and then centrifuge to obtain the precipitate 2 for use; Precipitate 2 was dissolved in 10% sodium chloride and stirred for 12 hours. Add 3 times the volume of NaCl solution with absolute ethanol, stir evenly, stand st...

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Abstract

The invention belongs to the water treatment field and discloses a bio-diatomite composite flocculant. The flocculant mainly contains refined diatomite and bioflocculant, wherein the weight of the bioflocculant is 10%-15% of that of refined diatomite; and the bioflocculant is an extracellular polymer prepared by selecting at least one of microbial strains with the preservation number CGMCC No. 3514, CGMCC No. 3515 and CGMCC No. 3516, fermenting, extracting, separating and purifying. The bio-diatomite composite flocculant is characterized by efficient flocculation, adsorption and filtration and environmental friend; and the flocculant can be directly added in the biochemical system of the existing sewage treatment system, thus industrial and engineering applications are easy to realize and the upgrading of the existing sewage treatment plants is also easy to realize.

Description

Technical field [0001] The invention belongs to the field of water treatment, and relates to a biological-diatomite composite flocculant, in particular to a biological-diatomite composite flocculant that combines a biological flocculant and diatomaceous earth for advanced sewage treatment. Background technique [0002] Flocculation is an important process in water supply safety purification and wastewater treatment, and flocculants directly affect the flocculation effect. Most of the inorganic flocculants currently on the market, such as aluminum and iron, are toxic and affect ecological safety; organic polymer flocculants such as polyacrylamide have neurotoxic, teratogenic, carcinogenic, and mutagenic effects, making traditional flocculants Applications are greatly restricted. [0003] Microbial flocculants have the advantages of non-toxic, safe, biodegradable, no secondary pollution, etc., and have great application potential in the fields of water purification and turbidity rem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F1/52C02F1/56C02F1/28
Inventor 李顺鹏蔺嘉梅吴效祥蒋建东邹敏李荣顾文柳王融戴昕郭燕
Owner NANJING AGRICULTURAL UNIVERSITY
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