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Photosensitive microcapsule used for photodynamic therapy and preparation method thereof

A technology of photodynamic therapy and microcapsules, which is applied in the field of photosensitive microcapsules and its preparation, can solve the problems of reducing the production of singlet oxygen, toxic and side effects of normal tissues, and the reduction of singlet oxygen production rate, so as to maintain high-efficiency singlet oxygen quantum The effects of yield, high singlet oxygen quantum yield, and easy control of permeability

Inactive Publication Date: 2011-07-27
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the above reports, small molecular photosensitive particles are randomly adsorbed on the capsule wall material or enriched inside the capsule. Such photosensitive particles will agglomerate with the increase of enrichment concentration, which will lead to a decrease in photosensitization efficiency or singlet oxygen production rate. reduce
In addition, the adsorbed small molecular photosensitive particles are easy to leak through the pores of the cyst wall before reaching the lesion site, reducing the production of singlet oxygen and easily causing toxic side effects to normal tissues

Method used

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  • Photosensitive microcapsule used for photodynamic therapy and preparation method thereof
  • Photosensitive microcapsule used for photodynamic therapy and preparation method thereof
  • Photosensitive microcapsule used for photodynamic therapy and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Preparation of photosensitive capsule wall material:

[0030] A: Add 0.1g of CHI into 25mL of 1% acetic acid aqueous solution by volume, stir at a stirring speed of 200-1000rpm for more than 12h, and adjust the pH to 5-6 with MES buffer;

[0031] B: Dissolve 0.028g of RB (Rose Bengal) in 25mL of anhydrous methanol solution, and add the catalyst 1-ethyl-3-(3-dimethylaminopropyl)-carbonization at a stirring speed of 200-1000rpm Diimide / N-hydroxysuccinimide, activate the carboxyl group for about 30 minutes;

[0032] C: slowly drop the CHI acetic acid solution in step A into the RB anhydrous methanol solution in step B, and at the same time stir at a stirring speed of 500-1000 rpm for 1-3 days, and control the temperature at 25-60°C;

[0033] D: Dialyze the reactant in step C with a dialysis bag with a molecular weight cut-off of 7000 for 5-20 days, wash / centrifuge 2-3 times, freeze-dry the obtained precipitate for 2-4 days, and obtain powdery pale pink Photopolymer produ...

Embodiment 2

[0048] Preparation of photosensitive capsule wall material:

[0049] A: Dissolve 0.5g of CHI in 50mL of 1% acetic acid solution, and adjust the pH to 5-6 with sodium carbonate / sodium bicarbonate buffer;

[0050] B: Dissolve 0.05g of fluorescein in 5mL of anhydrous methanol solution, and the stirring speed is 200-1000rpm;

[0051] C: slowly drop the CHI acetic acid solution in step A into the fluorescein anhydrous methanol solution in step B to react for more than 48 hours, and at the same time, obtain the reactant under vacuum at 60-80°C at a stirring speed of 500-1000 rpm;

[0052] D: Dialyze the reactant in step C with a dialysis bag with a molecular weight cut-off of 7000 for 5-20 days, wash / centrifuge 2-3 times, and freeze-dry the obtained precipitate for 1-2 days to obtain a powdery photosensitive polymer product.

[0053] E: Dissolve the powdery photosensitive polymer product in step D in deionized water, and prepare a photosensitive polymer aqueous solution with a con...

Embodiment 3

[0062] A: Dissolve 1 g of dextran in 40 mL of NaOH aqueous solution, and adjust the pH to 5-6 with MES buffer;

[0063] B: Dissolve 0.06g of 9-chloromethylanthracene in 2mL of toluene solution, and the stirring speed is 500-1000rpm;

[0064] C: slowly drop the dextran solution in step A into the 9-chloromethylanthracene solution in step B, and at the same time stir at a stirring speed of 100-600rpm for 1-3 days, in N 2 Under protection, control the temperature at 40-80°C to react;

[0065] D: Dialyze the reactant in step C with a dialysis bag with a molecular weight cut-off of 7000 for 5 to 20 days, wash / centrifuge for 2 to 3 times, and freeze-dry the precipitate obtained by extracting with cyclohexane for 1 to 2 days to obtain Powdered photopolymer product.

[0066] E: Prepare the photosensitive polymer in step D into a 1 mg / mL photosensitive polymer aqueous solution for use;

[0067] Preparation of photosensitive microcapsules:

[0068] A: Take 1mL of CaCO that has been ...

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Abstract

The invention discloses a photosensitive microcapsule used for photodynamic therapy and a preparation method thereof, belonging to the technical field of photosensitive polymer thin film materials. The photosensitive microcapsule is prepared by the following steps: firstly, preparing photosensitive polymer materials of photosensitizer molecule graft polymer, and then adopting the layer self assembly technology, assembling polyelectrolyte materials with opposite charges on a microballoon template, and preparing a hollow capsule assembled by a plurality of capsule walls, wherein materials of the capsule walls include natural polymer glycan (CHI) or glucan directly grafted with photosensitizer by chemical bonds. The photosensitive microcapsule has good biocompatibility, fixes photosensitive molecules, can kill cancer cells effectively, can be applied to (Photodynamic Therapy) PDT, and is good in water dispersion and uniform in particle size, high in single-state oxygen content yield under the illumination of beams with specific wavelength, simple in process and easy in operation.

Description

technical field [0001] The invention belongs to the technical field of photosensitive polymer film materials, and in particular relates to a photosensitive microcapsule which can be used for photodynamic therapy and a preparation method thereof. Background technique [0002] Cancer is one of the major diseases threatening human life. At present, the control and comprehensive treatment of cancer is the focus of national health strategies, and it is also a research hotspot that the world pays attention to. Traditional cancer treatment methods, namely surgery, radiotherapy and chemotherapy, have been continuously improved and can control and cure a variety of tumors, but there are still great defects. For example, surgery will seriously damage the patient's function or appearance, and radiotherapy and chemotherapy will have Larger toxic and side effects. [0003] Photodynamic therapy (PDT), which emerged in the 1990s, is a photochemical reaction that locally treats the lesion...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K41/00A61K47/36A61P35/00
Inventor 陶霞王晓磊郑言贞陈建峰
Owner BEIJING UNIV OF CHEM TECH
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