Method for extracting and separating bacitracin

Abstract

The invention relates to a method for extracting and separating bacitracin. The method is characterized by comprising the following steps of: (1) extracting and separating bacitracin concentrated liquor by ultrafiltration, namely acidizing bacitracin fermentation liquor, filtering or centrifuging by a vibrating screen to remove thalli, removing the thalli and macromolecular impurities by the ultrafiltration, removing an inorganic salt by resin adsorption and performing evaporation concentration under vacuum to obtain the bacitracin concentrated liquor; and (2) preparing a bacitracin crystalline solid by using the bacitracin concentrated liquor, namely performing production steps of extracting, decolorizing, nanofiltration, crystallizing and separating, drying, crushing, sieving, checking, the packaging of a finished product and the like on the bacitracin concentrated liquor to obtain the qualified bacitracin product. The method has the characteristics of high efficiency, simple equipment, high economy, large separation factor, high selectivity, low energy consumption, no secondary pollution, and the like, and is particularly suitable for the large-scale industrial production of the bacitracin; and operation can be performed continuously at the room temperature.

Description

Technical field: [0001] The invention relates to a method for separating and extracting bacitracin from bacitracin fermentation, in particular to a method for separating and extracting bacitracin from 5-20W / V% bacitracin fermentation broth. Background technique: [0002] Bacitracin (CAS1405-87-4) is a white or light yellow-gray powder, odorless and bitter. It is almost insoluble in water, methanol, acetone and chloroform, but easily soluble in pyridine and slightly soluble in ether. The substance is an unstable polypeptide composed of a variety of amino acids, containing A, A 1 , B, C and other at least 9 components of the mixture, wherein bacitracin A is the main active component. It was first discovered by B.H.Johnson in the United States in 1943. It can be extracted and isolated from the fermentation broth of Bacillus subtilis and Bacillus licheniformis. It is widely used to treat deep fungi, skin and mucosal fungi, etc. disease. [0003] Bacitracin is mainly prepared...

AI Technical Summary

Problems solved by technology

[0003] Bacitracin is mainly prepared by fermentation. There are many methods for extracting and separating bacitracin from fermentation broth, mainly including ion exchange method and organic solvent extraction method. U.S. patents US2776240 and US2915432 disclose the method of extracting and separating bacitracin by ion exchange adsorption method , the disadvantages of these methods are low yield and complex process

U.S. Patents US2498165 and US2609324 disclose methods for extracting and separating bacitracin with organic solvents. The organic solvents used in these methods are usually alcohols or various esters. The process is relatively simple, but due to the large amount of organic solvents used, the selectivity of the solvent Higher requirements are put forward for the regeneration ability, and the use of such toxic, explosive and volatile solvents has caused serious pollution to the environment and brought inconvenience to the operation.

At the same time, organic solvents are expensive, and their volatile characteristics also greatly increase the cost of industrial operation

[0004] Therefore, there are many extraction and separation methods for bacitracin, but there are many difficulties in applying these methods in industrial production.