Method for extracting and separating bacitracin

Abstract

The invention relates to a method for extracting and separating bacitracin. The method is characterized by comprising the following steps of: (1) extracting and separating bacitracin concentrated liquor by ultrafiltration, namely acidizing bacitracin fermentation liquor, filtering or centrifuging by a vibrating screen to remove thalli, removing the thalli and macromolecular impurities by the ultrafiltration, removing an inorganic salt by resin adsorption and performing evaporation concentration under vacuum to obtain the bacitracin concentrated liquor; and (2) preparing a bacitracin crystalline solid by using the bacitracin concentrated liquor, namely performing production steps of extracting, decolorizing, nanofiltration, crystallizing and separating, drying, crushing, sieving, checking, the packaging of a finished product and the like on the bacitracin concentrated liquor to obtain the qualified bacitracin product. The method has the characteristics of high efficiency, simple equipment, high economy, large separation factor, high selectivity, low energy consumption, no secondary pollution, and the like, and is particularly suitable for the large-scale industrial production of the bacitracin; and operation can be performed continuously at the room temperature.

Description

Technical field:

[0001] The invention relates to a method for separating and extracting bacitracin from bacitracin fermentation, in particular to a method for separating and extracting bacitracin from 5-20W / V% bacitracin fermentation broth. Background technique:

[0002] Bacitracin (CAS1405-87-4) is a white or light yellow-gray powder, odorless and bitter. It is almost insoluble in water, methanol, acetone and chloroform, but easily soluble in pyridine and slightly soluble in ether. The substance is an unstable polypeptide composed of a variety of amino acids, containing A, A 1 , B, C and other at least 9 components of the mixture, wherein bacitracin A is the main active component. It was first discovered by B.H.Johnson in the United States in 1943. It can be extracted and isolated from the fermentation broth of Bacillus subtilis and Bacillus licheniformis. It is widely used to treat deep fungi, skin and mucosal fungi, etc. disease.

[0003] Bacitracin is mainly prepared...