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Compound generated by marine Streptomyces sp. and application thereof

A compound, Streptomyces technology, applied in the biological field, can solve the problems of large metal ion residues, high energy consumption, low metal recovery efficiency, etc., and achieve the effect of high recovery efficiency and low energy consumption

Inactive Publication Date: 2013-03-20
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And limited by technical means, the ores mined are mostly limited to medium and high-grade ores, and a large number of ore sources with low grades for the "mechanical plus chemical method" and the remaining low-grade ores after mining medium and high-grade ores All the mines are on hold or even in a state of waste
[0004] Heavy metal ion wastewater from coal mines, iron ores, and metal sulfide mines, as well as heavy metal ion wastewater produced in other industrial processes, mainly uses physical and chemical methods such as natural purification, chemical precipitation, ion exchange, evaporation electrolysis, and polymer material adsorption. However, these methods all face problems such as high energy consumption, large metal ion residues, low recovery and utilization of metal ions, and methods that are not environmentally friendly.
[0005] The traditional method of using microbial conversion to treat heavy metal ions in ores and water mainly focuses on the change of the chemical state of metal ions by microorganisms through their own metabolic characteristics, resulting in high energy consumption, low yield, and low metal recovery efficiency.

Method used

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  • Compound generated by marine Streptomyces sp. and application thereof
  • Compound generated by marine Streptomyces sp. and application thereof
  • Compound generated by marine Streptomyces sp. and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1, the preparation of compound 8.012-1 and 8.012-2

[0074] A. Isolation of marine Streptomyces FXJ8.012

[0075] 1. Separation of strains

[0076] In October 2008, seawater samples were obtained from a depth of 3838 meters below the sea level of the Indian Ocean at 72.00 degrees east longitude and 37.83 degrees south latitude. The samples were concentrated and brought back to the laboratory for processing. Seawater samples were diluted with sterile water to make a suspension, pretreated by heating at 60°C for ten minutes, using ISP-3 (adding 3.3% sea salt) medium as the separation medium, and culturing at 28°C for 14 days. Single colonies were purified on GYM agar medium (glucose 4.0g, yeast extract 4.0g, malt extract 10.0g, calcium carbonate 2.0g, agar 15.0g, distilled water 1000.0ml) to obtain marine Streptomyces FXJ8.012.

[0077] 2. Morphological characteristics and physiological and biochemical identification of strains

[0078] 1. 16S rRNA analysis ...

Embodiment 2

[0148]Embodiment 2, compound 8.012-1 and 8.012-2 to metal ion Fe 3+ 、Cu 2+ and Zn 2+ Adsorption activity

[0149] Preliminary experiments found that compounds 8.012-1 and 8.012-2 quickly formed purple-red precipitates with ferric sulfate, ferric chloride, ferric citrate, and ferric phosphate; both quickly formed green precipitates with copper sulfate and copper chloride; Zinc sulfate and zinc chloride rapidly form white precipitates. It has no effect on the corresponding chromium salts, manganese salts, and lead salts.

[0150] 1. Preparation of experimental reagents

[0151] Take 1 mg of compound 8.012-1 and compound 8.012-2 obtained by the method 1 of step B obtained in Example 1, dissolve them in a small amount of DMSO, add them into test tubes containing 2 ml of sterile ultrapure water, and prepare 0.5 mg / ml Compound 8.012-1 solution and compound 8.012-2 solution; Ferric trichloride, copper sulfate and zinc sulfate were respectively prepared into 0.1mol / L standard sol...

Embodiment 3

[0158] Embodiment 3, produce the Streptomyces FXJ8.012 of compound 8.012-1 and compound 8.012-2 to metal ion Fe 3+ 、Cu 2+ adsorption

[0159] 1. Preparation of experimental reagents

[0160] The method preparation concentration according to one in embodiment 2 is that 0.1mol / L ferric chloride standard solution 100ml * 2 bottles (color is green), concentration is the copper sulfate standard solution 100ml * 2 bottles of 0.1mol / L (color is blue).

[0161] 2. Determination of the adsorption activity of Streptomyces FXJ8.012 on metal ions

[0162] Add 20g of dried mycelium of Streptomyces sp. FXJ8.012 CGMCCNo.4950 to each of the above 4 bottles of standard solution, shake at room temperature (25°C) for 4 hours, then centrifuge at 6000r / min for 15 minutes for solid-liquid separation, and find the solution The fading is obvious (that is, the color is sucked into the inside of the thallus by the bacteria, so that the color of the solution becomes lighter), indicating that Strepto...

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Abstract

The invention discloses a compound generated by marine Streptomyces sp. and an application thereof. The invention provides a compound shown in a formula I and also provides a method for preparing the compound. The method comprises the following steps: (1) fermenting Streptomyces sp. FXJ8.012 CGMCC (China General Microbiological Culture Collection Center) No.4950, collecting fermentation liquor and centrifuging to take mycelium; and (2) extracting the mycelium obtained in step (1) by ethanol, collecting extraction liquid, concentrating, drying and obtaining crude ethanol extract. The experiment of the invention proves that secondary metabolite compounds 8.012-1 and 8.012-2 generated in the cells of a bacterial strain are used to adsorb heavy metal ions in ore or a water body into the strain, and thus metal ions are enriched in the strain; and the strain is recovered so as to recover the heavy metal ions.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a compound produced by marine streptomyces and its application. Background technique [0002] Actinomycetes are a class of Gram-positive bacteria with high G+C mol% content of genomic DNA and complex morphological differentiation. They are named because their colonies are mostly radial. Actinomycetes can produce a variety of biologically active substances with high economic value, and are considered to be an important class of industrial microbial resources. About half of the more than 20,000 microbial-derived bioactive substances discovered so far are produced by actinomycetes. produced. [0003] At present, the vast majority of the metal mining industry in the world is carried out by mechanical and chemical methods, and the serious pollution caused by a large amount of dust, noise, toxic and harmful wastewater produced at the same time of mining has been universally recognized. A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P17/16C07D417/14C02F3/34C22B3/18C12R1/465C02F101/20
CPCY02P10/20
Inventor 黄英刘宁郗丽君张利敏
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI