Method for extracting whole peptidoglycan from bifidobacterium longum NQ-1501

A technology of Bifidobacterium longum and peptidoglycan, which is applied in pharmaceutical formulations, peptide/protein components, medical raw materials derived from bacteria, etc., can solve unfavorable large-scale process production and product quality control, large solvent usage, and production cycle The problem of lengthiness and other problems, to achieve suitable large-scale production, complete cell wall structure, good anti-tumor effect

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A technology of Bifidobacterium longum and peptidoglycan, which is applied in pharmaceutical formulations, peptide/protein components, medical raw materials derived from bacteria, etc., can solve unfavorable large-scale process production and product quality control, large solvent usage, and production cycle The problem of lengthiness and other problems, to achieve suitable large-scale production, complete cell wall structure, good anti-tumor effect

CN102321187AActive Publication Date: 2012-01-18内蒙古双奇药业股份有限公司

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Experimental program

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Effect test

Embodiment 1

[0036] Remove the fatty substances on the cell membrane by degreasing, and then extract the teichoic acid of Bifidobacterium longum NQ-1501 strain with trichloroacetic acid to increase the permeability of the cell membrane, which is conducive to the better effect of external substances on the cell content. The protein substances in the cells are removed by enzymatic hydrolysis solution, and finally the complete peptidoglycan obtained by centrifugation to remove impurities and then freeze-dried can be seen through transmission electron microscopy. The structure of the cell wall skeleton is complete. Bifidobacterium longum NQ-1501 strain is available from public sales of Inner Mongolia Shuangqi Pharmaceutical Co., Ltd.

[0037] The preparation method of the complete peptidoglycan comprises the following steps:

[0038] (1) Washing of bacteria:

[0039] Take 100 grams of wet thallus of Bifidobacterium longum NQ-1501 strain, add 1000 milliliters of physiological saline and mix ev...

Embodiment 2

[0051] Structural integrity detection of a method for extracting complete peptidoglycan from Bifidobacterium longum NQ-1501 disclosed by the present invention

[0052] The original bacterium and the prepared complete peptidoglycan in Example 1 of the present invention were detected by electron microscopy. The results are shown in Figures 1 and 2. The complete peptidoglycan still retains the complete external structure of the original bacteria.

Embodiment 3

[0054] Application of tabletting technology, using glucose, lactose, microcrystalline cellulose, skim milk powder and other auxiliary materials as raw materials to prepare granules, and then mixing complete peptidoglycan powder (obtained in Example 1) and magnesium stearate for tabletting, and finally obtaining long double Fidobacterium NQ-1501 Complete Peptidoglycan Tablets.

[0055] Its production method includes the following steps:

[0056] (1) Preparation of pharmaceutical excipients: 0.125 g of glucose, 0.05 g of microcrystalline cellulose, 0.033 g of sodium starch glycolate, 0.0025 g of sucrose, 0.003 g of citric acid, 0.0625 g of skimmed milk powder, and 0.1875 g of lactose.

[0057] (2) Mixing of raw materials: 34.4 g of complete peptidoglycan was mixed with the above-mentioned auxiliary materials. Then add 0.0015 grams of magnesium stearate, after mixing, the raw material for direct compression can be obtained.

[0058] (3) Tablet compression: Put the mixed raw mat...

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Abstract

The invention relates to a method for extracting whole peptidoglycan from bifidobacterium longum NQ-1501. The method comprises the following steps of removing fat-like substances on a cell membrane through a degreasing process, extracting teichoic acid to improve cell membrane permeability, removing protein-like substances in cells through an enzymolysis solution, carrying out centrifugation cleaning to remove impurities, and carrying out freeze-drying to obtain whole peptidoglycan. The whole peptidoglycan extracted by the method has a complete cell wall structure and has good antitumor effects. The method has the advantages of strong practicality, simple operation, short period, simple and reasonable processes, low cost, high yield, and good adaptability for large-scale production. The invention also relates to whole peptidoglycan prepared by the method and a medicinal preparation prepared from the whole peptidoglycan.

Description

technical field [0001] The invention relates to a method for extracting complete peptidoglycan from bifidobacterium longum NQ-1501, in particular to a method for applying a chemical method to extract complete peptidoglycan from probiotics. Background technique [0002] Cancer is one of the important diseases that threaten human life. The World Health Organization and the health departments of governments all regard conquering cancer as an important task. The research and development of new and efficient anti-tumor drugs not only has good economic benefits, but also brings huge social benefits. In general, anticancer drugs are very expensive due to the scarcity of raw materials or high technological content. Therefore, research and production of an anticancer drug with significant curative effect and low price is needed by society. [0003] Scholars at home and abroad have clearly clarified that Bifidobacterium longum is a physiologically beneficial bacterium, which plays a...

Claims

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Application Information

Patent Timeline

18 Jan 2012

Publication

CN102321187A

IPC: C08B37/00; A61K35/74; A61K38/16; A61P35/00; A61K35/745

Inventors: 黄少磊; 刘彦民