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Method for screening high efficiency activator of oil reservoir indigenous microbes

A technology of endogenous microorganisms and activators, applied in the fields of microbial biotechnology and resource and environmental biology, can solve the problems of different types and dosages of activator components in oil reservoirs, lack of screening process, and inability to quickly screen efficient activator components, etc. Achieve the effect of addressing the lack of random and standardized screening methods

Inactive Publication Date: 2012-05-02
NANKAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the difference in the number of endogenous microbial groups, community structure composition, nutrient demand characteristics, reservoir characteristics and development characteristics in different reservoir environments, the types and dosages of activator components in different reservoirs are different.
At present, the screening of activators at home and abroad is relatively random, lacks a systematic and scientific screening process, and cannot quickly screen high-efficiency activator components for specific oil reservoir blocks

Method used

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  • Method for screening high efficiency activator of oil reservoir indigenous microbes
  • Method for screening high efficiency activator of oil reservoir indigenous microbes
  • Method for screening high efficiency activator of oil reservoir indigenous microbes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Determination and Analysis of Inorganic Salt Ion Composition in Reservoir Production Liquid Water Phase

[0047] Referring to JY / T 015-1996 General Rules of Inductively Coupled Plasma Emission Spectrometry and HJ / T 84-2001 Determination of Inorganic Anions in Water Quality by Ion Chromatography, ion chromatography and inductively coupled plasma emission spectrometry were used to produce oil reservoirs in this block. The ion composition of the liquid and water phase inorganic salts was measured, and the results are shown in Table 1.

[0048] The measurement results show that the total salinity of dipped 3-N12 injected water is 8909.24 mg·l -1 , dipped in 3-X24 and the total salinity of the output liquid water phase is 8524.978mg·l -1 , are within the optimum growth range of microorganisms, and will not adversely affect the growth of microorganisms. Nitrogen and phosphorus nutrients are extremely lacking in each well, which cannot meet the growth needs of micr...

Embodiment 2

[0052] Example 2 Detection and Analysis of Endogenous Microbial Groups and Quantities in Reservoirs

[0053] Referring to the standard SY / T0532-93 "Oilfield Injection Water Bacteria Analysis Method Extinction Dilution Method", the number of endogenous microbial groups in the oil reservoir of block A well was detected and analyzed, and the results are shown in Table 2.

[0054] The measurement results show that the concentration of hydrocarbon oxidizing bacteria, nitrate reducing bacteria and methanogenic bacteria in well A is low, which are the target bacteria for activation; the concentration of sulfate reducing bacteria is as high as 10 2 cells·ml -1 , reflecting that there are a large number of sulfate-reducing bacteria in the oilfield water injection system and the internal environment of the oil reservoir, which need to be eliminated or inhibited from further growth. Hydrocarbon oxidizing bacteria can grow with petroleum hydrocarbons as the only carbon source, and metabo...

Embodiment 3

[0058] The screening of embodiment 3 inorganic nutrient salts

[0059] Common inorganic nitrogen and phosphorus nutrients mainly include potassium nitrate, sodium nitrate, ammonium nitrate, urea, ammonium chloride, ammonium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium dihydrogen phosphate, dihydrogen phosphate Ammonium and ammonium dihydrogen phosphate and sodium polyphosphate, etc. Among them, ammonium nitrate, ammonium chloride, ammonium sulfate, dipotassium hydrogen phosphate, disodium hydrogen phosphate, diammonium hydrogen phosphate and sodium polyphosphate aqueous solution are alkaline, and the pH value is between 8-9; potassium dihydrogen phosphate, phosphoric acid The aqueous solution of sodium dihydrogen and ammonium dihydrogen phosphate is acidic, with a pH value between 4-5. According to the pH of the formation water of the specific oil reservoir, the physical and chemical properties of various nutrien...

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Abstract

The invention discloses a method for screening a high efficiency activator of oil reservoir indigenous microbes. The method is suitable for oil reservoirs and zones subjected to long-term water-flooding exploitation. The method comprises the following steps of 1, carrying out detection analysis of types and the number of indigenous microbes in a desired oil reservoir, and the composition of water-phase inorganic salt ions of output liquid to determine oil reservoir indigenous microbe type composition and nutritional requirement characteristics, 2, determining an activator screening principle according to a result of the detection analysis and information of an activator source, an activator price, activator transportation and activator storage, 3, carrying out activator type screening according to the nutritional requirement characteristics and the activator screening principle, 4, carrying out primary quantifying of the activator selected by the step 3 according to the influences of activator ingredient content on an activation period, an application cost and pH values of oil and water samples, the compatibleness of activator ingredients and an oil reservoir output liquid water phase, solid water-insoluble substance content, and corrosivity, and 5, optimizing the activator ingredients and the activator ingredient content through a single factor experiment, an orthogonal experiment and a response surface experiment. The method provided by the invention is conducive to fast and efficient screening of a high-efficiency activator component suitable for a specific oil reservoir area.

Description

technical field [0001] The invention relates to a screening method for high-efficiency activators of endogenous microorganisms in oil reservoirs, and belongs to the fields of microbial biotechnology and resource environment biotechnology. Background technique [0002] The endogenous microbial community in the oil reservoir is the microbial community that enters the oil reservoir with the injected water during the oilfield water injection development process, and maintains a relatively stable number and type of microbial community in a certain period of time. These microbial metabolic types have many types and great variability. And the cycle of energy occupies an important position in the earth's ecosystem. Divided by metabolic type, endogenous microorganisms in oil reservoirs mainly include hydrocarbon oxidizing bacteria, saprophytic bacteria, anaerobic fermentation bacteria, nitrate-reducing bacteria, sulfate-reducing bacteria and methanogenic bacteria, etc. Some of these ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/28G01N21/71G01N30/96G01N33/48
Inventor 马挺高配科李国强赵玲侠曹美娜高梦黎
Owner NANKAI UNIV
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