Production method of medical biological material for human serum albumin

A technology of human serum albumin and its production method, which is applied in the field of preparation of porous sponge or film using human serum albumin, to achieve the effect of strong mechanical strength and strong ductility

Active Publication Date: 2014-03-05
HANGZHOU YAHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Biotechnol Prog. 27: 290-296; Jain S, Mathur R, Das M, Swarnakar NK, Mishra AK. 2011. Synthesis, pharmacocoscintigraphic evaluation and antitumor efficacy of methotrexate-loaded, folate-conjugated, stealth albumin nanoparticles. Nanomedicine (London) 6:1733-1754), but to our knowledge, the use of glutaraldehyde to use human serum albumin as a matrix material to produce porous materials or membranes has not been reported

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  • Production method of medical biological material for human serum albumin
  • Production method of medical biological material for human serum albumin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 0.5 g of recombinant human serum albumin (5%, w / v) was weighed and dissolved in 10 ml of sodium bicarbonate-sodium carbonate buffer (10 mM, pH 7.4) prepared from deionized water. Stir intermittently at 37°C for 1 hour to dissolve the protein. After the protein is completely dissolved and becomes a transparent liquid, use a vacuum pump for 30 minutes to quickly eliminate the bubbles in the solution. Slowly add 0.25% (v / v) glutaraldehyde to form a gel. The gel was washed 3 times with deionized water, 2 liters each time, for 3 hours to remove the buffer in the gel. Prepare 10% glycerol from deionized water, and infiltrate the albumin gel for 30 minutes. Place the albumin gel in a -70°C ultra-low temperature refrigerator to freeze for more than 4 hours, then put the frozen albumin gel into a freeze dryer, and vacuum-dry it for 24 hours to make a porous sponge without thrombin . The albumin porous sponge was sealed and stored dry at 4°C. This albumin porous sponge has ex...

Embodiment 2

[0037] 0.5 g (5%, w / v) of plasma-derived human serum albumin was weighed and dissolved in 10 ml of sodium bicarbonate-sodium carbonate buffer (10 mM, pH 7.4) prepared from deionized water. Stir intermittently at 37°C to dissolve it. After the protein is completely dissolved and becomes a transparent liquid, use a vacuum pump to pump air for 30 minutes to quickly eliminate the bubbles in the solution. Slowly add 0.25% (v / v) glutaraldehyde to form a gel. The gel was washed 3 times with deionized water, 2 liters each time, for 3 hours to remove the buffer in the gel. Prepare 10% glycerol from deionized water, and infiltrate the albumin gel for 30 minutes. Place the albumin gel in a -70°C ultra-low temperature refrigerator to freeze for more than 4 hours, then put the frozen albumin gel into a freeze dryer, and vacuum-dry it for 24 hours to make a porous sponge without thrombin . The performance of this sponge is slightly worse than that of the albumin sponge derived from gene ...

Embodiment 3

[0039] Weigh 1.0 g (10%, w / v) of human serum albumin produced by fermentation of genetically engineered yeast, and dissolve it in 10 ml of sodium phosphate buffer (10 mM, pH 7.4) prepared from deionized water. Stir intermittently at 37°C for 1 to dissolve the protein. After the protein is completely dissolved and becomes a transparent liquid, use a vacuum pump to pump air for 30 minutes to quickly eliminate the bubbles in the solution. Slowly add 0.125% (v / v) glutaraldehyde to form a gel. The gel was washed 3 times with deionized water, 2 liters each time, for 3 hours to remove the buffer in the gel. Prepare 10% glycerol from deionized water, and infiltrate the albumin gel for 30 minutes. Place the albumin gel in a -70°C ultra-low temperature refrigerator to freeze for more than 4 hours, then put the frozen albumin gel into a freeze dryer, and vacuum-dry it for 24 hours to make a porous sponge without thrombin . This albumin sponge has poor water absorption (12 times) with ...

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Abstract

The invention relates to a production method of a porous sponge or film for human serum albumin. The production method comprises the following steps: dissolving the human serum albumin into buffer solution with pH of 7.0-10.0, slowly adding glutaraldehyde, and adjusting the concentration of the glutaraldehyde to obtain human serum albumin gels with different degrees of cross linking; cleaning the gels to remove the buffer solution in the gel, infiltrating by 5-30% of glycerin or 5-30% of glycerin solution containing thrombin, freezing and drying to obtain the porous sponge or film for the human serum albumin containing or not containing the thrombin. According to the method provided by the invention, the prepared porous sponge or film for the human serum albumin can absorb blood on a surface of the wound to form a coagulation net rack through a porous structure thereof, so that the blood is stopped quickly; meanwhile, the production method has the characteristics of free antigenicity, good biocompatibility and ability of degrading, simulating cell division and promoting union, and has remarkable progress compared to the conventional medical materials.

Description

technical field [0001] The invention relates to a preparation method of human serum albumin medical biological material, in particular to a preparation method of using human serum albumin to produce porous sponge or film. Background technique [0002] Finding ideal medical biomaterials has always been the focus of scientists' research. Such biomaterials require biocompatibility, plasticity and stability to meet various medical requirements, including hemostatic sponges, artificial heart valves and blood vessels. Membranes, structural materials for artificial bone and artificial skin, and wound healing dressings, etc. [0003] At present, the technical principles of medical absorbable materials used clinically mainly adopt two types of natural absorbable materials and artificially synthesized absorbable materials. The former is mainly prepared from sheep intestines, collagen fibers, chitin (chitosan), etc. Glycolide, polylactic acid, polydioxanone, carboxymethyl fiber, etc. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/28C08J3/24C08J3/075C08L89/00C08K5/07C08J5/18A61L15/32A61L31/14
Inventor 朱明华
Owner HANGZHOU YAHUI BIOTECH CO LTD
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