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Microbial preparation method of dibutyl phthalate

A technology of dibutyl phthalate and microorganisms, which is applied in the field of preparation of dibutyl phthalate, can solve the problems of low sample recovery rate, high separation cost, small sample injection volume and the like, and achieves high separation efficiency, The effect of wide distribution system and large injection volume

Inactive Publication Date: 2012-10-03
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These studies all utilize high-performance liquid chromatography (HPLC), which uses a solid support. There are phenomena such as low sample recovery, denaturation, inactivation, and tailing caused by the adsorption of sample components. Small and expensive to separate

Method used

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  • Microbial preparation method of dibutyl phthalate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1.1 Selection of strains

[0029] The Verticillium lecani strain CGMCC NO.3.4505 (obtained from scale insects) used was purchased from the Culture Collection and Management Center of the Institute of Microbiology, Chinese Academy of Sciences, and stored in a 4°C refrigerator for later use.

[0030] 1.2 Culture of strains

[0031] 1.2.1 First-level slant seed culture

[0032] Put the strain with the preservation number CGMCC NO.3.4505 into a test tube containing PDA slant medium, put it in an incubator, and cultivate it for 7 days at a temperature of 25°C and a relative humidity of 75%. Mycelia will cover the slant and produce spores .

[0033] Incline medium: 200g potato, 20g agar, 20g glucose, 1000ml distilled water.

[0034] 1.2.2 Secondary liquid seed culture

[0035] Add about 40ml of liquid culture medium into a 100ml Erlenmeyer flask, and sterilize it under high pressure at 1.5pa / kg for 30 minutes. After cooling, inoculate the first-grade strains into the liqu...

Embodiment 2

[0055] 1.1 Selection of strains

[0056] The Verticillium lecani strain CGMCC NO.3.4504 (obtained from scale insects) used was purchased from the Culture Collection and Management Center of the Institute of Microbiology, Chinese Academy of Sciences, and stored in a 4°C refrigerator for later use.

[0057] 1.2 The cultivation of the bacterial strain is the same as in Example 1.

[0058] 1.3 The extraction of metabolites is the same as in Example 1.

[0059] 1.4 Separation and purification of the crude extract by high-speed countercurrent chromatography is the same as in Example 1.

[0060] 1.5 GC and GC / MS analysis are the same as in Example 1.

[0061] 2 results

[0062] GC / MS ion current chromatogram of colorless oily liquid produced by strain CGMCC NO.3.4504 ( Figure 7 ), the main component is DBP after searching the spectral library.

Embodiment 3

[0064] 1.1 Selection of strains

[0065] The used Beauveria tenella (CGMCCNO.2382) was isolated from the carcasses of Chinese pine forest caterpillars in Wafangdian Village, Shatuozi Township, Chengde County, Hebei Province. CGMCC), and stored in a refrigerator at 4°C for later use.

[0066] 1.2 The cultivation of the bacterial strain is the same as in Example 1.

[0067] 1.3 The extraction of metabolites is the same as in Example 1.

[0068] 1.4 Separation and purification of the crude extract by high-speed countercurrent chromatography is the same as in Example 1.

[0069] 1.5 GC and GC / MS analysis are the same as in Example 1.

[0070] 2 results

[0071] GC / MS ion current chromatogram of colorless oily liquid produced by strain CGMCC NO.2382 ( Figure 8 ), the main component is DBP after searching the spectral library.

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Abstract

The invention provides a microbial preparation method of dibutyl phthalate. The microbial preparation method of the dibutyl phthalate specifically comprises the following steps: performing strain liquid fermentation by using fungus Verticillium lecani or Beauveria tenella; extracting fermented liquid; and performing separation and purification by high-speed countercurrent chromatography to obtainthe dibutyl phthalate. The dibutyl phthalate is prepared by a microbial fermentation method; compared with a liquid-solid chromatography, the microbial preparation method of the dibutyl phthalate hasthe advantages that: sample preprocessing work is simplified, a solid supporting body is not used, irreversible adsorption, denaturation, inactivation, tailing and other phenomena of a sample component are avoided, the separation capability is strong, the separation efficiency is high and the separation time is short; and compared with a chemical synthesis method, the microbial preparation methodof the dibutyl phthalate is simple, low in cost and free of pollution in a production process.

Description

Technical field: [0001] The present invention relates to the preparation of dibutyl phthalate, which belongs to a microbial preparation method of dibutyl phthalate, more specifically a method for separating and purifying dibutyl phthalate produced by fungal metabolism by high-speed countercurrent chromatography. Butyl method. Background technique: [0002] Dibutyl Phthalate (DBP) is one of the phthalates and is widely used as an excellent plasticizer. Its structural formula is shown in the figure below. [0003] [0004] The industrial production of DBP is based on phthalic anhydride and butanol as raw materials. 2 SO 4 Under catalysis, carry out intermittent esterification at atmospheric pressure, and then carry out neutralization, distillation and decolorization processes. The traditional acidic catalyst has high activity, cheap and easy to obtain, mature process, and high product yield, but it also has a series of side reactions caused by the oxidative dehydration ...

Claims

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Application Information

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IPC IPC(8): C12P7/62C12N1/14C07C69/80C07C67/56C12R1/645
Inventor 高英熊琦张艳峰薛皎亮谢映平
Owner SHANXI UNIV
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