Method for extracting chlorogenic acid and hyperoside from lonicera japonica leaves

A technology of honeysuckle leaves and hyperin, which is applied in chemical instruments and methods, separation/purification of carboxylic acid esters, sugar derivatives, etc., can solve the problem that the content of hyperin is not high, only reaches 30%, and the production cost High-level problems, to achieve the effect of abundant reserves, energy saving, and low safety hazards

Inactive Publication Date: 2012-12-12
JISHOU UNIVERSITY
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Problems solved by technology

[0032] To sum up, the current high content of honeysuckle chlorogenic acid is mainly completed by crushing dried honeysuckle leaves, leaching, filtration, extraction or chromatography. The utilization rate of chlorogenic acid in the whole production process only reaches about 30%, mainly due to In the drying process of honeysuckle leaves, the loss is very large....

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Fresh honeysuckle leaves with a moisture content of about 80% are de-enzymed and minced with a tea roller degreening machine (Tea roller degreening machine 6CST-80 produced by Yueyang Agricultural Mechanization Research Institute), and 200kg of fresh honeysuckle leaves with a moisture content of 45% are added to the aqueous solution 1000L, extracted at room temperature for 4 hours, filtered twice with three layers of gauze and once with a ceramic membrane to obtain the filtrate, after solid-liquid separation, add granular activated carbon (provided by Gongyi Hengtai Filter Material Co., Ltd.) for 30 minutes, add 210g of ascorbic acid in Concentrate in vacuum at 50°C to 80L; absorb the concentrated solution on a DM130 macroporous resin column, collect the effluent and washing solution to obtain 240L of solution I; then concentrate the eluate to 20L, and then adjust the pH value with 0.01mol / L hydrochloric acid solution To about 3, add 95% ethanol solution to a final conce...

Embodiment 2

[0061] The fresh honeysuckle leaves with a moisture content of about 75% are de-enzymed and crushed with a tea drum de-greening machine (Tea drum de-greening machine 6CST-80 produced by the Yueyang Agricultural Mechanization Research Institute), and 400kg of fresh honeysuckle leaves with a water content of 48% are weighed and added to the water. 1800L, extract at room temperature for 4 hours, filter twice with three layers of gauze, and filter once with a ceramic membrane to obtain the filtrate. After solid-liquid separation, add granular activated carbon for treatment for 30 minutes, add 400g of ascorbic acid and vacuum concentrate at 50°C to 140L; Adsorb on DM130 macroporous resin column, collect the effluent and washing liquid to obtain 450L of solution I; the eluate is then concentrated to 50L, then 0.01mol / L hydrochloric acid solution is used to adjust the pH value to about 3, and 95% ethanol solution is added to the final The concentration was 50%, crystallized overnight ...

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Abstract

In order to make full use of local resources, also reduce extraction and purification equipment investment, effectively improve the yield of chlorogenic acid and hyperoside from lonicera japonica leaves, shorten the production time from collection till obtaining of the high content chlorogenic acid and hyperoside, as well as reduce the production costs of chlorogenic acid and hyperoside, the invention provides a purification method for extracting chlorogenic acid and hyperoside from fresh lonicera japonica leaves. The method employs enzyme deactivation treatment, hydrochloric acid extraction, filtration, extraction, macroporous adsorption resin chromatography, and crystallization so as to obtain pure chlorogenic acid and hyperoside products. The whole separation process has no high requirements for environmental conditions, the separation time is short, and the purity is high. Separation materials are easily available and cheap, and the separation operation process is simple and is easy to control. Crystallization and recrystallization are adopted. The purification efficiency is high, environmental pollution is reduced, and resources are also saved.

Description

Technical field: [0001] The invention belongs to the field of natural product processing, and relates to a method for extracting chlorogenic acid and hyperin from honeysuckle, more specifically to a method for extracting chlorogenic acid and hyperin from fresh leaves of honeysuckle Methods. Background technique [0002] Chlorogenic acid, also known as coffee tannic acid, is a condensed acid formed by caffeic acid and quinic acid. It belongs to phenylpropanoid compounds. It is a natural antioxidant produced by cells itself and an activator of cell metabolism. immunity. Studies in recent years have shown that chlorogenic acid has pharmacological effects on the digestive system, blood system and reproductive system, and has a wide range of anti-bacterial and anti-inflammatory, choleretic, hemostasis and increased white blood cell counts. It has a significant inhibitory effect on Staphylococcus aureus, hemolytic streptococcus, Shigella, and typhoid bacillus; it has a significa...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/48C07H17/07C07H1/08
Inventor 李兰岚王斯成代洪波洪剑锋
Owner JISHOU UNIVERSITY
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