Application of jasmonic acid methyl ester analogue serving as plant growth adjusting agent
A plant growth regulator, methyl jasmonate technology, applied in the direction of plant growth regulators, plant growth regulators, applications, etc., can solve problems such as difficult to achieve the best, to achieve improved stress resistance, high biological activity, and promote The effect of plant resistance
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Embodiment 1
[0037] Inhibitory effect of methyl jasmonate analogues such as formula (I) compounds on lettuce seed germination:
[0038] The preparation of the test solution is to dissolve the compounds 1, 2, 5, 7, 13, 17, and 21 in Table 1 in water, and at the same time, in order to increase the solubility of the compound in water and the uniformity of distribution, add 30mL of the compound per liter of the aqueous solution Dimethyl sulfoxide, which is made into different concentrations of plant culture fluid to treat lettuce seeds to carry out lettuce seed germination inhibition test, after 45 hours, measure its germination rate relative to the control (see Table 2) (germination rate = after adding number of germinated seeds / number of blank germinated seeds).
[0039] Table 2
[0040]
[0041] Compared with the control group, the synthetic methyl jasmonate analogs shown in the above table 2 have a better inhibitory effect on the germination of lettuce seeds, and the inhibition rate is...
Embodiment 2
[0043] Induction effect of methyl jasmonate analogs such as compounds of formula (I) on paclitaxel production in yew cells
[0044]The preparation of test solution is to dissolve compound 1,2,5,7,13,17,21 in table 1 in water, in order to increase the solubility and the uniformity of distribution of this type of plant growth regulator in water, every liter of compound aqueous solution Add 30mL dimethyl sulfoxide to the medium to make different concentrations, induce taxus cells paclitaxel, and use 100mL Erlenmeyer flasks for subculture. The control group is the subculture medium without adding plant growth regulators, and the experimental group is Add the subculture medium of above-mentioned different concentrations of plant growth regulators; Suspend the cells, put the fresh cells into a 100mL Erlenmeyer flask with an inoculation density of 12% after sterile suction filtration (add 4.8g of fresh cells to each Erlenmeyer flask containing 40mL medium), and then place the Erlenme...
Embodiment 3
[0049] Induction effect of methyl jasmonate analogs such as compounds of formula (I) on chlorophyll content of Ginkgo biloba leaves
[0050] The preparation of test solution is to dissolve compound 1,2,5,7,13,17,21 in table 1 in water, in order to increase the solubility and the uniformity of distribution of this type of plant growth regulator in water, every liter of compound aqueous solution Add 30mL dimethyl sulfoxide to it to make solutions with different concentrations, and use it as a culture medium to induce ginkgo leaves. Take 0.2 g ginkgo leaf sample after induction for 48 hours, add a small amount of quartz sand, and then add 10 mL of 80% acetone to grind it into a homogenate until the tissue turns white. The homogenate is centrifuged at a speed of 4000 r / min for 10 min in a centrifuge. Take the supernatant and measure its absorbance at wavelengths of 663nm and 645nm, and use 80% acetone as a blank control. According to the formula Ca=12.21OD 663 -2.81OD 645 , Cb=...
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